Abstract
Purpose: Our purpose was to investigate the associationbetween percentage chromomycin A3 (CMA3) positivity ofspermatozoa with some sperm parameters and in vitrofertilization rate.
Methods: Spermatozoa were collected from 139 men,washed in PBS, fixed in methanol/glacial acetic acid (3:1),and then spread on slides. CMA3 positivity is expressed asthe percentage in 200 spermatozoa.
Results: Percentage of CMA3 positivity showed not only anegative correlation with fertilization rate but also asignificant difference between fertilizing and nonfertilizingpatients. Moreover, percentage of CMA3-positivespermatozoa showed a negative correlation with count and percentagemotility and a positive correlation with percentage ofabnormal morphology. Percentage of CMA3 positivity also had apositive correlation with some abnormalities of head such asamorphous and macrocephaly. Ultrastructural study showedchromatin unpackaging in high CMA3-positive semensamples in comparison with low CMA3-positive semen samples.
Conclusion: There is a close relationship among fertilizationrate, sperm parameters, and CMA3 positivity and CMA3could be considered as a useful tool for evaluation of malefertility prior to infertility treatment.
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Iranpour, F.G., Nasr-Esfahani, M.H., Valojerdi, M.R. et al. Chromomycin A3 Staining as a Useful Tool for Evaluation of Male Fertility. J Assist Reprod Genet 17, 60–66 (2000). https://doi.org/10.1023/A:1009406231811
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DOI: https://doi.org/10.1023/A:1009406231811