Abstract
Purpose: A mouse model of unilateral oviductal obstruction was designed to study whether healthy oviducts can support embryo development in an advanced stage toward blastocyst hatching and implantation when the embryos in the contralateral side normally move into the uterine cavity.
Methods: The oviducts of 80 female ICR mice (aged 5–8 weeks) were ligated unilaterally 12–40 hr postcoitus. The ligated oviducts were isolated from day 4 to 19.5 postcoitus. Embryos within the ligated oviducts were then flushed out to record the developmental stage and compared with the conceptuses in the contralateral uterine horns with unligated oviducts. Embryos recovered from ligated oviducts were then cultured in vitro to observe their potential for further development.
Results: In 33 mice, 53.4% (163/305) and 86.1% (241/280) of the morphologically normal blastocysts had hatched from the zona pellucida within the obstructed tube and contralateral uterine horns, respectively, on the 5th day postcoitus. The data demonstrated that the hatching process could take place within the obstructed fallopian tube, but the timing was delayed. From 5.5 to 19.5 days postcoitus, a total of 362 implanted embryos were obtained in unligated control uterine horns, but none of the 404 embryos in the artificially obstructed oviducts were implanted. The embryos within the ligated tubes were dormant in the hatched blastocyst stage as demonstrated by their ability to continue growing (98.2%) when removed from the oviduct to an in vitro environment.
Conclusions: In this study, we demonstrate that mouse embryos can hatch, although delayed, in obstructed healthy oviducts. Tubal pregnancy is not likely to happen in artificially obstructed healthy mouse fallopian tubes, since all the viable embryos were dormant at the hatched blastocyst stage.
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REFERENCES
Harper MJK: Sperm and egg transport. In Reproduction in Mammals I. Germ Cells and Fertilization, 2nd ed, Austin CR, Short RV (eds). Cambridge, England, Cambridge University Press, 1982, pp. 102-127
Buhi WC, O'Brien B, Alvarez IM, Erdos G, Dubois D: Immunogold localization of porcine oviductal secretary proteins within the zona pellucida, perivitelline space, and plasmamembrane of oviductal and uterine oocytes and early embryos. Biol Reprod 1993;48:1274-1283
Bolton VN, Hawes SM, Taylor CT, Parsons JH: Development of spare human preimplantation embryos in vitro: An analysis of the correlations among gross morphology, cleavage rates, and development to the blastocyst. J In Vitro Fertil Embryo Transf 1989;6:30-35
Kiessling AA, Davis HW, Williams CS, Sauter RW, Harrison LW: Development and DNA polymerase activities in cultured preimplantation mouse embryos: Comparison with embryos developed in vivo. J Exp Zool 1991;258:34-47
Vlad M, Walker D, Kennedy RC: Nuclei number in human embryos co-cultured with human ampullary cells. Hum Reprod 1996;11:1678-1686
Confino E, Rawlins R, Binor Z, Radwanska E: The effect of the oviduct, uterine, and in vitro environments on zona thinning in the mouse embryo. Fertil Steril 1997;68:164-167
McLaren A: A study of blastocysts during delay and subsequent implantation in lactating mice. J Endocr 1968;42: 453-463
Aitken RJ: Changes in the protein content of mouse uterine flushings during normal pregnancy and delayed implantation, and after ovariectomy and oestradiol administration. J Reprod Fertil 1977;50:29-36
Finn CA, Martin L: The control of implantation. J Reprod Fertil 1974;39:195-206
McLaren A: Blastocyst activation. In The Regulation of Mammalian Reproduction, Segal SJ, Crozier R, Corfman PA et al. (eds). Thomas, Springfield, IL, 1973; pp 321-328
Psychoyos A: Endocrine control of egg implantation. In Handbook of Physiology, Section 7, Vol. 11, Part 2, Greep RO, Astwood EB (eds). 1973; pp 201-256
Aitken RJ: The culture of mouse blastocyst in the presence of uterine flushings collected during normal pregnancy, delayed implantation and pro-oestrus. J Embryol Exp Morphol 1977;41:295-300.
O'Neill C, Quinn P: Inhibitory influence of uterine secretions on mouse blastocysts decreases at the time of blastocyst activation. J Reprod Fertil 1983;68:269-274
Finn CA, Downie JM: Changes in the endometrium of mice after the induction of implantation by actinomycin D. J Endocrinol 1975;65:259-264
Camus M, Lejeune B, Leroy F: Induction of implantation in the rat by intraparametrial injection of actinomycin D. Biol Reprod 1979;20:1115-1118
Katagiri S, Yuen BH, Moon YS, Takahashi Y, Kanagawa H: Separation of a 170-K delayed-implantation-associated protein with inhibitory activity on trophoblast outgrowth and RNA synthesis by mouse embryo. Am J Reprod Immunol 1994;31:141-150
Katagiri S, Takahashi Y, Kanagawa H, Yuen BH, Moon YS: A delayed-implantation-associated protein prevents resumption ofDNAsynthesis by the trophectoderm of delayed-implanting mouse blastocysts in vitro. J Vet Med Sci 1998;60:791-794
Adams CE: Egg development in the rabbit: the influence of postcoital ligation of the uterine tube and of ovariectomy. J Endocrinol 1958;42:453-463
Murray FA, Jr, Bazer FW, Rundell JW, Vingent CK, Wallace HD, Warnick AG: Developmental failure of swine embryos restricted to the oviduct environment. J Reprod Fertil 1971;24:445-448
Pauerstein CJ, Eddy CA, Koong MK, Moore GD: Rabbit endosalpinx suppresses ectopic implantation. Fertil Steril 1990;54:522-526
Moore GD, Eddy CA, Pauerstein CJ: Rabbit endosalpinx inhibits implantation in vitro. Fertil Steril 1992;57:902-907
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Lee, R.KK., Lin, SP., Tsai, YJ. et al. Embryonic Dormancy Phenomenon in Obstructed Healthy Mouse Fallopian Tubes. J Assist Reprod Genet 17, 540–545 (2000). https://doi.org/10.1023/A:1009402126770
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DOI: https://doi.org/10.1023/A:1009402126770