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Constant expression of mouse calpastatin isoforms during differentiation in myoblast cell line, C2C12

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Abstract

C2C12 is a myoblast cell line which is used to studydifferentiation into multinucleated cells in vitro. Addition of calpain inhibitors, calpeptin orE-64d, to the culture medium prevented the myoblasticfusion of C2C12 cells. Immunoblot studies usingaffinity-purified antibody, revealed that the expressedlevels of mouse calpastatin remained unaltered duringC2C12 cell fusion. The detected calpastatin migratedas a protein of 130 kDa on SDS-polyacrylamide gelelectrophoresis. The estimated molecular mass wassomewhat greater than that in mouse liver anderythrocytes, and much greater than that reported inrat myoblasts. The 130 kDa isoform may contain anadditional N-terminal region designated XL domainfound in bovine calpastatin.

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Authors and Affiliations

  1. Lab of Molecular and Cellular Regulation, Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan

    Kiyotaka Hitomi, Maki Murase & Toshimi Kawamura

  2. Lab of Molecular and Cellular Regulation, Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan

    Masatoshi Maki

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  1. Kiyotaka Hitomi
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  2. Maki Murase
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  3. Toshimi Kawamura
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  4. Masatoshi Maki
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Hitomi, K., Murase, M., Kawamura, T. et al. Constant expression of mouse calpastatin isoforms during differentiation in myoblast cell line, C2C12. Cytotechnology 33, 63–70 (2000). https://doi.org/10.1023/A:1008194002923

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