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Cytotechnology

, Volume 33, Issue 1–3, pp 63–70 | Cite as

Constant expression of mouse calpastatin isoforms during differentiation in myoblast cell line, C2C12

  • Kiyotaka Hitomi
  • Maki Murase
  • Toshimi Kawamura
  • Masatoshi Maki
Article

Abstract

C2C12 is a myoblast cell line which is used to studydifferentiation into multinucleated cells in vitro. Addition of calpain inhibitors, calpeptin orE-64d, to the culture medium prevented the myoblasticfusion of C2C12 cells. Immunoblot studies usingaffinity-purified antibody, revealed that the expressedlevels of mouse calpastatin remained unaltered duringC2C12 cell fusion. The detected calpastatin migratedas a protein of 130 kDa on SDS-polyacrylamide gelelectrophoresis. The estimated molecular mass wassomewhat greater than that in mouse liver anderythrocytes, and much greater than that reported inrat myoblasts. The 130 kDa isoform may contain anadditional N-terminal region designated XL domainfound in bovine calpastatin.

calpain calpastatin myoblast C2C12 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Kiyotaka Hitomi
    • 1
  • Maki Murase
    • 1
  • Toshimi Kawamura
    • 1
  • Masatoshi Maki
    • 2
  1. 1.Lab of Molecular and Cellular Regulation, Department of Applied Molecular Biosciences, Graduate School of Bioagricultural SciencesNagoya UniversityChikusa, NagoyaJapan
  2. 2.Lab of Molecular and Cellular Regulation, Department of Applied Molecular Biosciences, Graduate School of Bioagricultural SciencesNagoya UniversityChikusa, NagoyaJapan

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