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Cytotechnology

, Volume 34, Issue 1–2, pp 131–139 | Cite as

Prolongation of murine hybridoma cell survival in stationary batch culture by Bcl-xL expression

  • Joel R. Charbonneau
  • Eric R. GauthierEmail author
Article

Abstract

While the ectopic expression of the anti-apoptoticprotein Bcl-2 has been shown to significantly increaseboth cell viability and antibody production in batchculture, some cell lines are refractory to thesemanipulations. For example, the NS/O and theP3x63Ag8.653 murine myelomas, which express highendogenous levels of the Bcl-2 homologue Bcl-xL, areboth resistant to the anti-apoptotic effect of Bcl-2.This indicates that, in these cells, Bcl-2 and Bcl-xLmay be functionally redundant. In order to define therole which Bcl-xL plays in hybridoma cultures, we usedthe Sp2/0-Ag14 cell line. This murine hybridomaexpresses low levels of Bcl-xL and is highly sensitiveto apoptosis induction by cycloheximide (CHX) and byamino acid depletion. Bcl-xL-transfected Sp2/0-Ag14cells were more resistant than the wild type and theplasmid-containing cells to apoptosis induced by CHXand by glutamine depletion. Moreover, when compared tothe vector-transfected control, Bcl-xL-Sp2/0 cellsexhibited a substantial increase in viability instationary batch culture. Interestingly, Sp2/0-Ag14cells overexpressing Bcl-xL showed a growth behaviourthat was similar to the parent myeloma cell lineP3x63Ag8.653. Our results suggest that Bcl-xLexpression levels are sufficient to account for therelative robustness of some hybridoma cell lines instationary batch cultures.

apoptosis bcl-xL cell growth cell viability hybridoma myeloma 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  1. 1.Cellular Biochemistry Research Laboratory, Department of Chemistry and BiochemistryLaurentian UniversityOntarioCanada
  2. 2.Cellular Biochemistry Research Laboratory, Department of Chemistry and BiochemistryLaurentian UniversityOntarioCanada

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