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Cytotechnology

, Volume 34, Issue 1–2, pp 11–15 | Cite as

Induction of Malathion resistance in CCE/CC128 cell line of Mediterranean fruit fly (Ceratitis capitata (Wied.)) (Diptera: Tephritidae)

  • Elisabetta Rossi
  • Giuseppe Rainaldi
Article

Abstract

The CCE/CC128 cell line, derived from fertilized eggs of theMediterranean fruit fly (Ceratitis capitata), was used toinvestigate whether insect cells in culture could developresistance to Malathion. After 20 cycles of pulse-chasetreatment (28 h exposure to 90 μg/ml of Malathion and 48 hrecovery in normal medium), a Mal 90 selected population wasobtained. DNA content analysis showed that the values were distributed between levels 2C and 4C and no accumulation ofcells in a specific phase of the cell cycle was observed.Furthermore, preliminary molecular analysis showed noamplification of the esterase gene in resistant cells.Cross-resistance of Mal 90 cells towards other insecticides wasassayed and found to be absent. Our data support the idea thatthe medfly cell line and, more generally, insect cell cultures,could represent a promising system to investigate insecticideresistance mechanisms.

insecticide resistance Malathion Mediterranean fruit fly 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Elisabetta Rossi
    • 1
  • Giuseppe Rainaldi
    • 2
  1. 1.Dipartimento Coltivazione e Difesa delle Specie LegnoseSez. Entomologia AgrariaPisaItaly
  2. 2.Istituto di Mutagenesi e DifferenziamentoGenetica e Biochimica TossicologicaPisaItaly

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