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Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin

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Abstract

The ratio of hapten and bovine serum albumin in an antigenconjugate was determined by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Ahybridoma secreting monoclonal antibody against ginsenosideRg1 was produced by fusing sprenocytes immunized with aginsenoside Rg1-bovine serum albumin conjugate withHAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very smallcross-reaction appeared with ginsenoside Re. The full measuringrange of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 ofginsenoside Rg1.

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Authors and Affiliations

  1. Department of Pharmacognosy, Graduate school of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higahsi-ku, Fukuoka, 812,-8582, Japan

    Noriko Fukuda & Hiroyuki Tanaka

  2. Department of Pharmacognosy, Graduate school of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higahsi-ku, Fukuoka, 812,-8582, Japan

    Yukihiro Shoyama

Authors
  1. Noriko Fukuda
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  2. Hiroyuki Tanaka
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  3. Yukihiro Shoyama
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Correspondence to Yukihiro Shoyama.

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Fukuda, N., Tanaka, H. & Shoyama, Y. Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin. Cytotechnology 34, 197–204 (2000). https://doi.org/10.1023/A:1008162703957

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