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Cytotechnology

, Volume 34, Issue 1–2, pp 121–130 | Cite as

The construction of an in vitro three-dimensional hematopoietic microenvironment for mouse bone marrow cells employing porous carriers

  • Yoshiaki Tomimori
  • Mutsumi TakagiEmail author
  • Toshiomi Yoshida
Article

Abstract

Spatial development of mouse bone marrow cellsemploying porous carriers was investigated in order todesign a bioreactor with a three-dimensionalhematopoietic microenvironment. Three types of porouscarriers were used for examining the spatialdevelopment of anchorage-dependent primary stromalcells as feeder cells. Stromal cells were found tospread well at a high density on a polyester nonwovendisc carrier (Fibra cel (FC)) under a scanningelectron microscope, while cells on porous cellulosebeads (Microcube (MC), 500 μm pore diameter)spread at a low density; cells on another type ofcellulose porous beads (CPB, 100 μm pore diameter)were globular. Mouse bone marrow cells wereinoculated to dishes containing three types of porouscarriers which shared more than 30% of the bottomsurface in a dish. The concentration of stromal cellsin the well containing FC was lower than that on theother two carriers. However, the weekly output oftotal hematopoietic cell (suspension cells) increasedbetween day 21 and 28 in the culture using FC while itdecreased monotonously in the cultures by use of theother two carriers. The proportion of progenitorcells (BFU-E, CFU-GM) in the total hematopoietic cellpopulation, after showing an initial decrease,increased after 1 week in the culture using FC whilethe proportion decreased monotonously to zero in thecultures using MC and CPB.

bone marrow nonwoven fabric porous carriers spatial development stromal cell 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Yoshiaki Tomimori
    • 1
  • Mutsumi Takagi
    • 2
    Email author
  • Toshiomi Yoshida
    • 1
  1. 1.International Center for BiotechnologyOsaka UniversityOsakaJapan
  2. 2.International Center for BiotechnologyOsaka UniversityOsakaJapan

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