, Volume 33, Issue 1–3, pp 117–122 | Cite as

Micropattern-immobilization of heparin to regulate cell growth with fibroblast growth factor

  • Yong Soon Park
  • Yoshihiro Ito


Heparin was immobilized on a polystyrene plate in a specificpattern by photolithography. Heparin was coupled with azidoaniline. Thederivatized heparin was cast on the polystyrene plate from aqueoussolution. After drying, the plate was photo-irradiated in the presence of aphotomask. The micropatterning was confirmed by staining with a dye,ethydium bromide. Since heparin has negative charges, the cationic dyewas adsorbed on the regions where heparin was immobilized. In thepresence fibroblast growth factor (FGF), the growth of mouse fibroblastSTO cells was enhanced only on the heparin-immobilized regions. Thisresult indicated that micropattern-immobilized heparin activated FGF forcell growth activity.

biomaterial cell growth fibroblast growth facor heparin pattern-immobilization 


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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Yong Soon Park
    • 1
  • Yoshihiro Ito
    • 2
    • 3
  1. 1.Graduate School of Materials ScienceNAISTIkomaJapan
  2. 2.Graduate School of Materials ScienceNAISTIkomaJapan
  3. 3.Department of Biological Science and Technology, Faculty of EngineeringThe University of TokushimaTokushimaJapan

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