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Immunochemical characteristics of a novel cell death receptor and a decoy receptor on granulosa cells of porcine ovarian follicles

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Abstract

Previously, we prepared an IgM monoclonal antibody(PFG-1) which specifically recognized a cell-membraneglycoprotein (PFG-1 antigen; 55 kD, pI 5.9),immunohistochemically reacted with granulosa cells ofhealthy follicles but not of atretic follicles, andinduced granulosa cell apoptosis. In the presentstudy, an IgM monoclonal antibody (PFG-3) capable ofinducing granulosa cell apoptosis and an IgGmonoclonal antibody (PFG-4) not capable of inducingapoptosis were produced against granulosa cellsprepared from healthy antral follicles of porcineovaries. Two-dimensional Western blotting analysisrevealed that PFG-3 specifically recognized twocell-membrane proteins (named PFG-3-1 andPFG-3-2/PFG-1 antigens; 42 kD, pI 5.2 and 55 kD, pI5.9, respectively) of healthy granulosa cells, andthat PFG-4 recognized the same two cell-membraneproteins. In atretic granulosa cells, PFG-3-2/PFG-1antigen disappeared. Immunochemical reactions of theseantibodies were only detected in follicular granulosacells but not any other ovarian tissues or organs.PFG-3 and PFG-4 immunohistochemically reacted withgranulosa cells of healthy and atretic follicles. Whenthe isolated granulosa cells prepared from healthyfollicles were cultured in medium containing PFG-3,the cells underwent apoptosis, and co-incubation withPFG-4 inhibited PFG-3-inducible apoptosis. Theseobservations suggested that PFG-3-2/PFG-1 antigen isa novel cell death receptor which is different fromthe apoptosis-mediating receptors (Fas/Apo-1/CD95 orTNF receptor), and that PFG-3-1 antigen may act as adecoy receptor and inhibit apoptotic signal transmission.

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Authors and Affiliations

  1. Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto, 606-8502, Japan

    Noboru Manabe

  2. Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto, 606-8502, Japan

    Akira Myoumoto, Chiemi Tajima, Mizuho Nakayama, Kozue Uchio, Misuzu Yamaguchi & Hajime Miyamoto

  3. Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, 980-8575, Japan

    Manabu Fukumoto

Authors
  1. Noboru Manabe
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  2. Akira Myoumoto
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  3. Chiemi Tajima
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  4. Manabu Fukumoto
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  5. Mizuho Nakayama
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  6. Kozue Uchio
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  7. Misuzu Yamaguchi
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  8. Hajime Miyamoto
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Correspondence to Noboru Manabe.

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Manabe, N., Myoumoto, A., Tajima, C. et al. Immunochemical characteristics of a novel cell death receptor and a decoy receptor on granulosa cells of porcine ovarian follicles. Cytotechnology 33, 189–201 (2000). https://doi.org/10.1023/A:1008146119761

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