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Hydroxyapatite-pulp composite fiber sheet bed: A new material for the immobilization of CHO-K1 cells

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Abstract

A new immobilization material for cell culture, ahydroxyapatite-pulp composite fiber (HAPC) sheet bed, was usedto grow CHO-K1 cells. The sheet bed for cell culture wasprepared from HAPC fiber by paper-making techniques. Scanning electron microscopic analysis revealed that the HAPCsheet bed had a structure consisting of piled fibers with spaces 10–200 μm in diameter and a pore surface area of 0.32 m2 g-1. Using a 25 × 25 mm2 squareHAPC sheet bed 0.41 mm in thickness (85 g m-2 basis weight) for cell culture, CHO-K1 cells grew to a cell densityof 3.7 × 107 cells cm-3 in a 60 mm plastic dish over a 6-day culture period. High-density culture of CHO-K1 cells was successfully performed using the HAPC sheet bed in a 500 ml spinner flask over a 21-day culture period. The HAPC sheet bed, wound around the stirrer paddle, was rotated in the spinner flask in order to supply nutrientsand remove waste products efficiently. The HAPC sheet bedhas a large surface area to support cell growth and there islarge diffusion space inside of the bed. This newautoclavable substrate for anchorage-dependent cells can be easily scaled-up.

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Authors and Affiliations

  1. Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume-shi, Fukuoka, 839-0861, Japan

    Hironobu Kawakatsu

  2. Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume-shi, Fukuoka, 839-0861, Japan

    Seiji Ide & Yasumasa Okuda

  3. Graduate School of Genetic Resources Technology, Kyusyu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Sanetaka Shirahata

Authors
  1. Hironobu Kawakatsu
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  2. Seiji Ide
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  3. Yasumasa Okuda
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  4. Sanetaka Shirahata
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Kawakatsu, H., Ide, S., Okuda, Y. et al. Hydroxyapatite-pulp composite fiber sheet bed: A new material for the immobilization of CHO-K1 cells. Cytotechnology 35, 65–72 (2001). https://doi.org/10.1023/A:1008144305744

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  • DOI: https://doi.org/10.1023/A:1008144305744

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