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Cytotechnology

, Volume 33, Issue 1–3, pp 123–130 | Cite as

Treatment of mouse melanoma cells with phorbol 12-myristate 13-acetate counteracts mannosylerythritol lipid-induced growth arrest and apoptosis

  • Xiaoxian Zhao
  • Christian Geltinger
  • Shotaro Kishikawa
  • Kiyoshi Ohshima
  • Takehide Murata
  • Nobuhiko Nomura
  • Tadaatsu Nakahara
  • Kazunari K. YokoyamaEmail author
Article

Abstract

Mannosylerythritol lipid (MEL), an extracellularglycolipid from yeast, induces the differentiation ofHL-60 promyelocytic leukemia cells towardsgranulocytes. We show here that MEL is also a potentinhibitor of the proliferation of mouse melanoma B16cells. Flow-cytometric analysis of the cell cycle ofMEL-treated B16 cells revealed the accumulation ofcells in the sub-G0/G1 phase, which is a hallmark ofcells undergoing apoptosis. Treatment of B16 cellsfor 24 h with phorbol 12-myristate 13-acetate (PMA),an activator of protein kinase C (PKC), did notinterfere with the growth and survival of the cells,but it effectively counteracted the MEL-induced growtharrest and apoptosis. The activity of PKC was reducedin B16 cells treated with MEL at a concentration atwhich MEL induced apoptosis. However, incubation withPMA in addition to MEL reversed this reduction in theactivity of PKC. These results suggest thatconverging signaling pathways are triggeredindependently by MEL and PMA and that the signalsmight both be mediated by PKC.

apoptosis mannosylerythritol lipid melanoma protein kinase C 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Xiaoxian Zhao
    • 1
    • 2
  • Christian Geltinger
    • 1
  • Shotaro Kishikawa
    • 1
  • Kiyoshi Ohshima
    • 1
  • Takehide Murata
    • 1
  • Nobuhiko Nomura
    • 2
  • Tadaatsu Nakahara
    • 2
  • Kazunari K. Yokoyama
    • 3
    Email author
  1. 1.Tsukuba Life Science CenterRIKEN (The Institute of Physical and Chemical Research)IbarakiJapan
  2. 2.Institute of Applied BiochemistryUniversity of TsukubaIbarakiJapan
  3. 3.Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research)IbarakiJapan

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