, Volume 34, Issue 1–2, pp 151–158 | Cite as

Justification of continuous packed-bed reactor for retroviral vector production from amphotropic ΨCRIP murine producer cell

  • Seung-Hyun Kang
  • Byung-Gee Kim
  • Gyun Min Lee


To indentify a plausible large-scale production system forretroviral vector, three culture systems, i.e., batch culturewith medium exchange, microcarrier culture, and packed-bedreactor culture were compared. In batch cultures with mediumexchange, high cell concentrations were maintained for about amonth, and the harvested retroviral titer remained constant. Inmicrocarrier cultures, although cell growth was rapid, theretroviral titer was unexpectedly low, suggesting that the lowtiter was due either to serious damage to the retroviral vectoror to a reduction in the production rate of retroviral vector,caused by mechanical shear forces. Although the retroviral titer(maximum titer, 1.56 × 106) in the packed-bedreactor was a little bit lower than that obtained in the batchculture with medium exchange (maximum titer, 1.91 ×106), continuous production made it possible to increasethe cumulative titer up to 16-fold of that from the batchculture with medium exchange. Moreover, as the packed-bedreactor system requires less labor and shows excellentvolumetric productivity in comparison to batch cultures withmedium exchanges, it will be an appropriate production systemfor retroviral vector in large quantities.

anchorage-dependent cell cell culture packed-bedreactor retroviral vector viral production 


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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Seung-Hyun Kang
    • 1
    • 2
  • Byung-Gee Kim
    • 1
    • 2
  • Gyun Min Lee
    • 3
  1. 1.School of Chemical Engineering, Institute for Molecular Biology and GeneticsSeoul National UniversitySeoulKorea
  2. 2.Institute for Molecular Biology and GeneticsSeoul National UniversitySeoulKorea
  3. 3.Department of Biological SciencesKorea Advanced Institute of Science and TechnologyTaejonKorea

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