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Cytotechnology

, Volume 33, Issue 1–3, pp 109–115 | Cite as

Chromosomal assignment of the chicken NP220 gene encoding a large DNA-binding nuclear protein and its targeted disruption in chicken DT40 cells

  • Yuichi Matsushima
  • Kiyoshi Matsumura
  • Shohji Ishii
  • Tomohiro Suzuki
  • Yoichi Matsuda/snm>
  • Yasuo KitagawaEmail author
Article
  • 24 Downloads

Abstract

NP220s form a family of DNA-binding nuclear proteinsoriginally found in human cell lines. Four isoformsof NP220 are produced in humans and mice probably byalternative splicing of two sequence units. Theyhave RNA-recognition and arginine/serine rich motivescommonly found in many nuclear proteins important forpre-mRNA processing. In order to analyze the functionof NP220s, its gene was disrupted in chicken cell lineDT40. For this, gemomic DNA clone of chicken NP220was isolated and the gene was localized tochromosome 4q2.7–q2.8. Chicken NP220 conserves MH1and zinc finger-like motives found in human and mouseNP220s. Despite its expression as a mRNA of 6 kb inwild type DT40 cells, disruption of the NP220gene did not impair the growth of DT40 cells.

chicken gene chromosomal mapping matrin 3 NP220 nuclear matrix targeted integration 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Yuichi Matsushima
    • 1
  • Kiyoshi Matsumura
    • 2
  • Shohji Ishii
    • 2
  • Tomohiro Suzuki
    • 3
  • Yoichi Matsuda/snm>
    • 3
  • Yasuo Kitagawa
    • 1
    • 2
    Email author
  1. 1.Nagoya University BioScience Center, Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan
  2. 2.Graduate Program of Biochemical Regulation, Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan
  3. 3.Laboratory of Animal Genetics, Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan

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