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Cytotechnology

, Volume 31, Issue 1–2, pp 69–75 | Cite as

Formation of cylindrical multicellular aggregate (cylindroid) and expression of liver specific functions of primary rat hepatocytes

  • Hiroshi Mizumoto
  • Masashi Hayakami
  • Kohji Nakazawa
  • Hiroyuki Ijima
  • Kazumori Funatsu
Article

Abstract

In our studies of the development of a hybrid artificial liver, we investigated the formation of cylindrical multicellular aggregate (cylindroid) of primary rat hepatocytes on a pressed sheet of polyurethane foam (pressed–PUF) as a culture substratum. Hepatocytes formed cylindroids by attaching to a pressed–PUF surface, peeling off from the surface and aggregating. The diameter and length of most cylindroids were approximately 200–500 μm and 500 μm–2 mm, respectively. The activities of liver specific functions (albumin secretion and ammonia metabolism) of hepatocyte cylindroids were equivalent to or higher than those of hepatocyte spheroids. These results suggest that hepatocyte cylindroids can maintain highly differentiated functions longer than hepatocyte spheroids, and that a PUF/cylindroid culture may be effective to develop of a hybrid artificial liver.

multicellular aggregate polyurethane foam primary culture rat hepatocytes 

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References

  1. Crane LJ and Miller DL (1977) Plasma protein synthesis by isolated rat hepatocytes. J Cell Biol 72: 11–25.CrossRefGoogle Scholar
  2. Gerlach JC, Encke J, Hole O, Müller C, Courtney JM and Neuhaus P (1994) Hepatocyte culture between three dimensionally arranged biomatrix-coated independent artificial capillary systems and sinusoidal endothelial cell co-culture compartments. Int J Artif Organs 17, 301–306.Google Scholar
  3. Ijima H, Nakazawa K, Mizumoto H, Matsushita T and Funatsu K (1998) Formation of a spherical multicellular aggregate (spheroid) of animal cells in the pores of polyurethane foam as a cell culture substratum and its application to a hybrid artificial liver. J Biomater Sci Polymer Ed 9: 765–778.Google Scholar
  4. Koide N, Sakaguchi K, Koide Y, Asano K, Kawaguchi M, Matsushima H, Takenami T, Shinji T, Mori M and Tsuji T (1990) Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp Cell Res 186: 227–235.CrossRefGoogle Scholar
  5. Matsushita T, Ijima H, Koide N and Funatsu K (1991) High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam. Appl Microbiol Biotechnol 36: 324–326.CrossRefGoogle Scholar
  6. Matsushita T, Ketayama M, Kamihara K and Funatsu K (1990) Anchorage-dependent mammalian cell culture using polyurethane foam as a new substratum for cell attachment. Appl Microbiol Biotechnol 33: 287–290.CrossRefGoogle Scholar
  7. Peshwa MV, Wu FJ, Sharp HL, Cerra FB and Hu WS (1996) Mechanistics of formation and ultrastructural evaluation of hepatocytes spheroids. In Vitro Cell Dev Biol-Animal 32: 197–203.Google Scholar
  8. Roberts RA and Somes AR (1993) Hepatocytes spheroids: Prolonged hepatocytes viability for in vitro modeling of nongenotoxic carcinogenesis. Fundam Appl Toxicol 21, 149–158.CrossRefGoogle Scholar
  9. Rozga J, Holzman MD, Ro M-S, Griffin DW, Neuzil DF, Giorgio T, Moscioni AD and Demetriou AA (1993) Development of a hybrid bioartificial liver. Ann Surg 217, 502–511.Google Scholar
  10. Wezel AL van (1967) Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nature 216: 64–65.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Hiroshi Mizumoto
  • Masashi Hayakami
  • Kohji Nakazawa
  • Hiroyuki Ijima
  • Kazumori Funatsu

There are no affiliations available

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