Abstract
Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. α1, β1 and γ1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. α4 and α5 mRNAs were additionally expressed after attachment of blastocysts to culture dishes. α2 and β2 mRNAs started to be expressed at the stage of allantois formation. Among ten laminin chains, only the mRNA encoding components of laminin-5 (α3, β3 and γ2) was missing in embryos up to the stage of allantois formation.
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Aberdam D, Aguzzi A, Baudoin C, Galliano MF, Ortonne JP and Meneguzzi G (1994) Developmental expression of nicein adhesion protein (laminin-5) subunits suggests multiple morphogenic roles. Cell Adhes Commun J 2: 115–129.
Beck K, Hunter I and Engel J (1990) Structure and function of laminin: anatomy of a multidomain glycoprotein. FASEB J 4: 148–160.
Bernier SM, Utani A, Sugiyama S, Doi T, Polistina C and Yamada Y (1994) Cloning and expression of laminin α2 chain (M-chain) in the mouse. Matrix Biol 14: 447–455.
Burgeson RE, Chiquet M, Deutzmann R, Ekblom P, Engel J, Kleinman H, Martin GR, Meneguzzi G, Paulsson M, Sanes J, et al. (1994) A new nomenclature for the laminins. Matrix Biol 14: 209–211.
Champliaud MF, Lunstrum GP, Rousselle P, Nishiyama T, Keene DR and Burgeson RE (1996) Human amnion contains a novel laminin variant, laminin 7, which like laminin 6, covalently associates with laminin 5 to promote stable epithelial-stromal attachment. J Cell Biol 132: 1189–1198.
Cooper AR and MacQueen HA (1983) Subunits of laminin are differentially synthesized in mouse eggs and early embryos. Dev Biol 96: 467–471.
Durkin ME, Gautam M, Loechel F, Sanes JR, Merlie JP, Albrechtsen R and Wewer UM (1996) Structural organization of the human and mouse laminin β2 chain genes, and alternative splicing at the 5′ end of the human transcript. J Biol Chem 271: 13407–13416.
Dziadek M and Timpl R (1985) Expression of nidogen and laminin in basement membranes during mouse embryogenesis and in teratocarcinoma cells. Dev Biol 111: 372–382.
Engel J (1993) in: Molecular and cellular aspects of Basement Membrane, Rohrbach DH and Timpl R (eds) pp. 147–176.
Engel J (1992) Laminin and other strange proteins. Biochemistry 31: 10643–10651.
Engel J, Odermatt E, Engel A, Madri JA, Furthmayr H, Rohde H and Timpl R (1981) Shapes, domain organizations and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrix. J Mol Biol 150: 97–120.
Ferrigno O, Virolle T, Galliano MF, Chauvin N, Ortonne JP, Meneguzzi G and Aberdam D (1997) Murine laminin alpha3A and alpha3B isoform chains are generated by usage of two promoters and alternative splicing. J Biol Chem 272: 20502–20507.
Galliano MF, Aberdam D, Aguzzi A, Ortonne JP and Meneguzzi G (1995) Cloning and complete primary structure of the mouse laminin alpha 3 chain. Distinct expression pattern of the laminin alpha 3A and alpha 3B chain isoforms. J Biol Chem 270: 21820–21826.
Hsu YC (1979) In vitro development of individually cultured whole mouse embryos from blastocyst to early somite stage. Dev Biol 68: 453–461
Iivanainen A, Kortesmaa J, Sahlberg C, Morita T, Bergmann U, Thesleff I and Tryggvason K (1997) Primary structure, developmental expression, and immunolocalization of the murine laminin alpha 4 chain. J Biol Chem 272: 27862–27868.
Leivo I, Vaheri A, Timpl R and Wartiovaara J (1980) Appearance and distribution of collagens and laminin in the early mouse embryo. Dev Biol 76: 100–114
Libbus BL and Hsu YC (1980) Sequentional development and tissue organization in whole mouse embryos cultured from blastocyst to early somite stage. Anat Rec 197: 317–329.
Liu J and Mayne R (1996) The complete cDNA coding sequence and tissue-specific expression of the mouse laminin alpha 4 chain. Matrix Biol 15: 433–437.
Martin GR, Timpl R and Kuhn K (1988) Basement membrane proteins: molecular structure and function. Adv Protein Chem 39: 1–50.
Masunaga T, Shimizu H, Ishiko A, Tomita Y, Aberdam D, Ortonne JP and Nishikawa T (1996) Localization of laminin-5 in the epidermal basement membrane. J Histochem Cytochem 44: 1223–1230.
Miner JH, Lewis RM and Sanes JR (1995) Molecular cloning of a novel laminin chain, alpha 5, and widespread expression in adult mouse tissues. J Biol Chem 270: 28523–28526.
Miner JH, Patton BL, Lentz SI, Gilbert DJ, Snider WD, Jenkins NA, Copeland NG and Sanes JR (1997) The laminin alpha chains: expression, developmental transitions, and chromosomal locations of alphal-5, identification of heterotrimeric laminins 8–11, and cloning of a novel alpha 3 isoform. J Cell Biol 137: 685–701.
Nadijcka M and Hillman N (1974) Ultrastructure studies of the mouse blastocyst substages. J Embryol Exp Morphol 32: 675–695.
Niimi T, Kumagai C, Okano M and Kitagawa Y (1997) Differentiation-dependent expression of laminin-8 (alpha 4 beta 1 gamma 1) mRNAs in mouse 3T3-L1 adipocytes. Matrix Biol 16: 223–230
Paulsson M, Dziadek M, Suchanek C, Huttner WB and Timpl R (1985) Nature of sulphated macromolecules in mouse Reichert's membrane. Evidence for tyrosine O-sulphate in basement membrane proteins. Biochem J 231: 571–579.
Poelmann RE and Mentink MM (1982a) Parietal yolk sac in early gestation mouse embryos: structure and function. Bibl Anat 22: 123–127.
Poelmann RE and Mentink MM (1982b) The maternal-embryonic barrier in the early post-implantation mouse embryo: a morphological and functional study. Scan Electron Microsc Pt 3: 1237–1247.
Sasaki M, Kato S, Kohno K, Martin GR and Yamada Y (1987) Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats. Proc Natl Acad Sci USA 84: 935–939.
Sasaki M, Kleinman HK, Huber H, Deutzmann R and Yamada Y (1988) Laminin, a multi-domain protein: the A chain has a unique domain and homology with the basement membrane proteoglycan and laminin B chains. J Biol Chem 263: 16536–16544.
Sasaki M and Yamada Y (1987) The laminin B2 chain has a multidomain homologous to the B1 chain. J Biol Chem 262: 17111–17117.
Schuler F and Sorokin L (1995) Expression of laminin isoforms in mouse myogenic cells in vitro and in vivo. J Cell Sci 108: 3795–3805.
Sugiyama S, Utani A, Yamada S, Kozak CA and Yamada Y (1995) Cloning and expression of the mouse laminin γ2 (B2t) chain, a subunit of epithelial cell laminin. Eur J Biochem 228: 120–128.
Sunada Y, Bernier SM, Kozak CA, Yamada Y and Campbell KP (1994) Deficiency of merosin in dystrophic dy mice and genetic linkage of laminin M chain gene to dy locus. J Biol Chem 269: 13729–13732.
Tamura RN, Oda D, Quaranta V, Plopper G, Lambert R, Glaser S and Jones JC (1997) Coating of titanium alloy with soluble laminin-5 promotes cell attachment and hemidesmosome assembly in gingival epithelial cells: potential application to dental implants. J Periodontal Res 32: 287–294.
Tokida Y, Aratani Y, Morita A and Kitagawa Y (1990) Production of two variant laminin forms by endothelial cells and shift of their relative levels by angiostatic steroids. J Biol Chem 265: 18123–18129.
Timpl R (1989) Structure and biological activity of basement membrane proteins. Eur J Biochem 180: 487–502.
Utani A, Kopp JB, Kozak CA, Matsuki Y, Amizuka N, Sugiyama S and Yamada Y (1995) Mouse kalinin B1 (laminin β3 chain): cloning and tissue distribution. Lab Invest 72: 300–310.
Vidal F, Baudoin C, Miquel C, Galliano MF, Christiano AM, Uitto J, Ortonne JP and Meneguzzi G (1995) Cloning of laminin alpha 3 chain gene (LAMA3) and identification of a homozygous deletion in a patient with Herlitz junctional epidermolysis bullosa. Genomics 30: 273–280.
Wu TC, Wan YJ, Chung AE and Damjanov I (1983) Immunohistochemical localization of entactin and laminin in mouse embryo and fetuses. Dev Biol 100: 496–505
Xu H, Christmas P, Wu XR, Wewer UM and Engvall E (1994) Defective muscle basement membrane and lack of M-laminin in the dystrophic dy/dy mouse. Proc Natl Acad Sci USA 91: 5572–5576.
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Azimi, M., Niimi, T., Yoshida, N. et al. Differential expression of mRNAs encoding laminin chain variants during in vitro development of mouse blastocysts. Cytotechnology 31, 185–193 (1999). https://doi.org/10.1023/A:1008084508489
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DOI: https://doi.org/10.1023/A:1008084508489