, Volume 31, Issue 1–2, pp 133–141 | Cite as

In vitro immunization of human peripheral blood lymphocytes: establishment of B cell lines secreting IgM specific for cholera toxin B subunit from lymphocytes stimulated with IL-2 and IL-4

  • Akira Ichikawa
  • Yoshinori Katakura
  • Kiichiro Teruya
  • Shuichi Hashizume
  • Sanetaka Shirahata


In vitro immunization (IVI) techniques have a great potential in the production of human monoclonal antibodies (MAbs) against various antigens. An IVI method of human peripheral blood lymphocytes (PBL) has been developed with a human lung adenocarcinoma cell line in our laboratory. Although several cancer specific human MAbs were successfully generated by using this IVI method, it was not available for soluble antigens, which prompted us to improve the method for generation of human MAbs against soluble antigens. IVI with soluble antigens was effectively caused by the addition of muramyl dipeptides, interleukin-2 and interleukin-4. It was found that the difference of sensitivity of lymphocytes depending upon donors could be overcome by finding the optimal concentrations of IL-2 and IL-4. IVI of human PBL was performed with cholera toxin B subunit (CTB) and the immunized B cells were transformed by Epstein-Barr virus. Anti-CTB antibody was detected using an indirect ELISA. B cells producing anti-CTB antibodies were directly cloned by a soft agar cloning method.

cholera toxin B subunit human monoclonal antibody interleukin-2 interleukin-4 in vitro immunization 


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© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Akira Ichikawa
  • Yoshinori Katakura
  • Kiichiro Teruya
  • Shuichi Hashizume
  • Sanetaka Shirahata

There are no affiliations available

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