Advertisement

Cytotechnology

, Volume 31, Issue 1–2, pp 153–158 | Cite as

Rapid separation of solasodine glycosides by an immunoaffinity column using anti-solamargine monoclonal antibody

  • Waraporn Putalun
  • Hiroyuki Tanaka
  • Yukihiro Shoyama
Article

Abstract

Immunoaffinity column using anti-solamargine monoclonal antibody for separation of solasodine glycosides was established. This method was specific for solasodine glycosides which was detected by thin layer chromatography and the western blotting. Total solasodine glycosides have been separated directly from the crude extract of Solanum khasianum fruit by the newly established immunoaffinity column.

immunoaffinity column MALDI-MS monoclonal antibody solasodine glycosides 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Goto Y, Shima Y, Morimoto S, Shoyama Y, Murakami H, Kusai A and Mojima K (1994) Determination of tetrahydrocanabinolic acid-carrier protein conjugate by matrix-assisted laser desorption/ionization mass spectrometry and antibody formation. Org Mass Spectrom 29, 668–671.CrossRefGoogle Scholar
  2. Ishiyama M, Shoyama Y, Murakami H and Shinohara H (1996) Production of monoclonal antibodies development of an ELISA for solamargine. Cytotechnology 18, 153–158.CrossRefGoogle Scholar
  3. Langone JJ (1982) Use of protein A to immunoglobulins from serum in hybridoma culture media. J Immunol Methods 51, 33–45.CrossRefGoogle Scholar
  4. Mahato SB, Sahu NP, Ganguly AN, Kasai R and Tanaka O (1980) Steroidal alkaloids from Solanum khasianum: application of 13C NMR spectroscopy to their structural elucidation. Phytochemistry 19, 2018–2020.CrossRefGoogle Scholar
  5. Sakata R, Shoyama Y, Murakami H (1994) Production of monoclonal antibodies of enzyme immunoassay for typical adenylatecyclase activator, forskolin. Cytotechnology 16, 101–108.CrossRefGoogle Scholar
  6. Shoyama Y, Sakata R, Isobe R and Murakami H (1993a) Direct determination of forskolin-bovine serum albumin conjugate by matrix assisted laser desorption ionization. Org Mass Spectrom 28, 987–988.CrossRefGoogle Scholar
  7. Shoyama Y, Fukuda T, Tanaka T, Kusai A and Nojima K (1993b) Direct determination of opium alkaloid-bovine serum albumin conjugate by matrix assisted laser desorption ionization mass spectrometry. Biol Pharm Bull 16, 1051–1053.Google Scholar
  8. Taki T, Kasama T, Handa S and Ishikawa P (1994) A simple and quantitative purification of glycosphingolipids and phospholipids by thin-layer chromatography. Anal Biochem 223, 232–238.CrossRefGoogle Scholar
  9. Tanaka H, Putalun W, Tsuzaki C and Shoyama Y (1997) A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine. FEBS Letters 404, 279–282.CrossRefGoogle Scholar
  10. Weiler EW, Kruger H and Zenk MH (1976) Radioimmunoassay for the determination of the steroidal alkaloid solasodine and related compounds in living plants and herbarium specimens. Planta Med 39, 112–124.CrossRefGoogle Scholar
  11. Yanagihara H, Sakata R, Minami H, Tanaka H, Shoyama Y and Murakami H (1996) Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application. Anal Chim Acta 335, 63–70.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Waraporn Putalun
  • Hiroyuki Tanaka
  • Yukihiro Shoyama

There are no affiliations available

Personalised recommendations