Abstract
A continuous process of insect cell (S f9) growth and baculovirus infection is tested with the sequential combination of a CSTR and a tubular reactor. A tubular infection reactor enables continuous introduction of baculovirus and therefore avoids the ‘passage effect’ observed in two-stage CSTR systems. Moreover, a tubular reactor can be used to test cell infection kinetics and the subsequent metabolism of infected insect cells. Unlike batch and CSTR culture, cells in a horizontally positioned tubular reactor settle due to poor mixing. We have overcome this problem by alternately introducing air bubbles and media and by maintaining a linear velocity sufficient to keep cells suspended. This article addresses the development of the tubular reactor and demonstrates its use as an infection system that complements the two-stage CSTR.
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Hu, YC., Wang, MY. & Bentley, W.E. A tubular segmented-flow bioreactor for the infection of insect cells with recombinant baculovirus. Cytotechnology 24, 143–152 (1997). https://doi.org/10.1023/A:1007970020274
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DOI: https://doi.org/10.1023/A:1007970020274