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Growth Stimulation of Tumor-Specific Cytotoxic T Lymphocytes on Concanavalin A-Immobilized Carrier Beads

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Abstract

Human tumor-specific CD4+ cytotoxic T lymphocytes (CTL) were generated against duodenum papilloma cell line TGBC18TKB from HLA type-matched peripheral blood mononuclear cells. Concanavalin A (Con A) immobilized on carrier beads stimulated growth of the CTL in a long-term culture without repeated antigen stimulation, while soluble Con A induced death of the CTL. The CTL exhibited the target-specific cytotoxicity in a more potent manner than those before the long-term culture in the presence of the immobilized Con A. Enhanced expression of the adhesion molecule, CD11b, was observed on the CTL. These results suggest that immobilized Con A will be useful for continuous growth stimulation and large scale expansion of CTL without tumor antigen.

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Authors and Affiliations

  1. RIKEN Cell Bank, The Institute of Physical and Chemical Research (RIKEN), Koyadai 3-1-1, Tsukuba Science City, 305, Japan

    Shu Qin Liu & Tadao Ohno

  2. Orquest, Inc., 365 Ravendale Drive, Mountain View, CA, 94043, U.S.A

    Lin-Shu Liu

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  1. Shu Qin Liu
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  2. Lin-Shu Liu
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  3. Tadao Ohno
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Liu, S.Q., Liu, LS. & Ohno, T. Growth Stimulation of Tumor-Specific Cytotoxic T Lymphocytes on Concanavalin A-Immobilized Carrier Beads. Cytotechnology 26, 13–21 (1998). https://doi.org/10.1023/A:1007924430570

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