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Effect of temperature on vegetative growth among isolates of Metarhizium anisopliae and M. flavoviride

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Abstract

Effects of temperature on vegetative growth on a semi-synthetic medium of 22 isolates of Metarhizium anisopliae and 14 isolates of M. flavoviride were determined. The majority of isolates of both species grew between 11 and 32°C; several isolates grew at 8 and 37 °C. None of the isolates grew at 40 °C. Relative growth rate, calculated from the maximum growth rate for each isolate, was significantly affected by temperature and isolate, with significant isolate * temperature interactions. The maximum absolute growth rates among the isolates ranged from 2.5 mm to 5.9 mm/day. Optimal temperatures were generally between 25 and 32 °C with several isolates exhibiting optimal growth at temperatures as high as 32 °C. Overall, relative growth rates were greater in isolates of M. anisopliae than M. flavoviride at temperatures of 25 °C or lower; conversely mean relative growth rates were greater in M. flavoviride than M. anisopliae at temperatures higher than 25 °C. However, the two most cold tolerant isolates at 8 °C were M. flavoviride and the three most heat tolerant at 35 °C were M. anisopliae. Since temperature growth responses varied considerably between isolates, strain selection according to thermal tolerance may be warranted when choosing a strain for development as a microbial control agent.

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Authors and Affiliations

  1. Unité de Recherche en Lutte Biologique, INRA-Montpellier, Campus International de Baillarguet, 34982, Montferrier-sur-Lez, Cedex, France

    A. Ouedraogo & J. Fargues

  2. Agriculture & Agri-Food Canada Research Centre, P.O. Box 3000, Lethbridge, Alberta, Canada, T1J 4B1

    M.S. Goettel

  3. IITA Biological Control Center for Africa, B.P. 08-0932, Cotonou, Benin

    C.J. Lomer

Authors
  1. A. Ouedraogo
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  2. J. Fargues
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  3. M.S. Goettel
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  4. C.J. Lomer
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Ouedraogo, A., Fargues, J., Goettel, M. et al. Effect of temperature on vegetative growth among isolates of Metarhizium anisopliae and M. flavoviride . Mycopathologia 137, 37–43 (1997). https://doi.org/10.1023/A:1006882621776

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  • DOI: https://doi.org/10.1023/A:1006882621776

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