Abstract
Ten microsatellite loci (Omy27DU,Omy325(A3)UoG, OmyFGT5TUF,OmyFGT14TUF, OmyFGT15TUF,OmyFGT23TUF, Omy77DU,Ssa20.19NUIG, Ots1BML, andOne18ASC) were amplified using the polymerase chain reaction to create genetic profiles for nine cell lines (RTG-2, RTH-149,RTL-W1,RTgill-W1, RTS-11, RTS-34st, RTP-2, RTP-91E and RTP-91F) from rainbow trout(Oncorhynchus mykiss) and one cell line (CHSE-214) from Chinook salmon (O. tschawytscha). A cell line (PHL) from anon-salmonid, the Pacific herring (Clupea harengus pallasi), was included as a control. The ten loci clearly revealed the uniqueness of each cell line, except for two cell lines (RTP-91E andRTP-91F) from the same fish. RTP-91E and RTP-91F were identical at all loci except Ssa20.19NUIG. The most useful locus for demonstrating uniqueness was Ots1BML. The information was used to demonstrate that an uncharacterized rainbow trout cell line (Clone 1A)was in fact CHSE-214, illustrating the usefulness of multiplexed microsatellites for the creation of genetic profiles for salmonid cell lines and for the testing of cell line cross-contamination.
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Babich H. and Borenfreund E. 1991. Cytotoxicity and genotoxicity naphassays with cultured fish cells: a review. Toxic. In Vitro. 5: 91–100.
Banks M.A., Blouin M.S., Baldwin B.A., Rashbrook V.K., Fitzgerald H.A., Blankenship S.M. et al. 1999. Isolation and inheritance of novel microsatellites in chinook salmon (Oncorhynchus tschawytscha). J. Hered. 90: 281–288.
Bardakci F. and Skibinski D.O.F. 1994. Application of the RAPD technique in tilapia fish: species and subspecies identification. Heredity 73: 117–123.
Bols N.C. 1991. Biotechnology and aquaculture: The role of cell cultures. Biotech. Adv. 9: 31–49.
Bols N.C. and Lee L.E.J. 1993. Cell lines: availability, propagation and isolation. In: Hochachka P.W. and Mommsen T.P. (eds), Biochemistry and Molecular Biology of Fishes Vol. 3. Elsevier, Amsterdam, pp. 145–159.
Bols N.C., Barlian A., Chirino-Trejo S.J., Caldwell S.J., Goegan P. and Lee L.E.J. 1994. Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss, gills. J. Fish Dis. 17: 601–611.
Bols N.C., Yang B.Y., Lee L.E. and Chen T.T. 1995. Development of a rainbow trout pituitary cell line that expresses growth hormone, prolactin, and somatolactin. Mol. Mar. Biol. Biotechnol. 4: 154–163.
Bols N.C., Whyte J., Clemons J., Tom D., van den Heuvel M. and Dixon D.G. 1997. Use of liver cell lines to develop toxic equivalency factors and to derive toxic equivalent concentrations in environmental samples. In: Zelikoff J.T. (ed.), Ecotoxicology: Responses, Biomarkers and Risk Assessment. SOS Publications, Fair Haven, pp. 329–350.
Dirks W., MacLeod R.A., Jager K., Milch H. and Drexler H.G. 1999. First searchable database for DNA profiles of human cell lines: sequential use of fingerprint techniques for authentication. Cell. Mol. Biol. 45: 841–853.
Ferrero M., CastaÑo A., Gonzalez A., Sanz F. and Becerril C. 1998. Characterization of RTG-2 fish cell line by random amplified polymorphic DNA. Ecotoxicol. Environ. Saf. 40: 56–64.
Ferguson M.M. and Danzmann R.G. 1998. Role of genetic markers in fisheries and aquaculture: useful tools or stamp collecting? Can. J. Fish Aquat. Sci. 55: 1553–1563.
Fishback A.G., Danzmann R.G., Sakamoto T. and Ferguson M.M. 1999. Optimization of semi-automated microsatellite multiplex Polymerase Chain Reaction systems for rainbow trout (On-corhynchus mykiss). Aquaculture 172: 247–254.
Fryer J.L. and Lannan C.N. 1994. Three decades of fish cell culture: a current listing of cell lines derived from fishes. J. Tissue Cult. Meth. 16: 87–94.
Fryer J.L., McCain B.B. and Leong J.C. 1981. A cell line derived from rainbow trout (Salmo gairdneri) hepatoma. Fish Pathol. 15: 193–200.
Ganassin R.C. and Bols N.C. 1998. Development of a monocyte/macrophage-like cell line, RTS11, from rainbow trout spleen. Fish & Shellfish Immunol. 8: 457–476.
Ganassin R.C. and Bols N.C. 1999. A stromal cell line from rainbow trout spleen, RTS34ST, that supports the growth of rainbow trout macrophages and produces conditioned medium with mitogenic effects on leukocytes. In Vitro Cell. Dev. Biol. 35: 80–86.
Ganassin R.C., Sanders S.M., Kennedy C.J., Joyce E.M. and Bols N.C. 1999. Development and characterization of a cell line from Pacific herring,Clupea harenguspallasi, sensitive to both naphthalene cytotoxicity and infection by viral hemorrhagic septicemia virus. Cell. Biol. Toxicol. 15: 99–309.
Ganassin R.C., Schirmer K. and Bols N.C. 2000. Cell and tissue culture. In: Ostrander K.G. (ed.), The Laboratory Fish. Academic Press, New York, pp. 631–651.
Greene A.E., Goldner H. and Coriell L.L. 1966. The species identification of poikilothermic tissue culture cells by the cyto toxic antibody test. Growth 30: 305–313.
Heath D.D., Pollard S. and Herbinger C.M. Genetic differentiation and isolation by distance in steelhead trout (Oncorhynchus mykiss) populations in British Columbia. Heredity (in press).
Hightower L.E. and Renfro J.L. 1988. Recent applications of fish cell culture to biomedical research. J. Exp. Zool. 248: 290–302.
Kaplan J. and Hukku B. 1998. Cell line characterization and authentication. Methods Cell. Biol. 57: 203–216.
King B., Lichtenstein A., Berenson J. and Kacinski B.M. 1994. Polymerase chain reaction-based microsatellite typing assay used for tumor cell line identification. Amer. J. Path. 144: 486–491.
Lannan C.N. 1994. Fish cell culture: a protocol for quality control. J. Tissue Cult. Meth. 16: 95–98.
Lannan C.N., Winton J.R. and Fryer J.L. 1984. Fish cell lines: Establishment and characterization of nine cell lines from salmonids. In Vitro 20: 671–676.
Lee L.E.J., Clemons J.H., Bechtel D.G., Caldwell S.J., Han K.B., Pasitschniak-Arts M. et al. 1993. Development and characterization of a rainbow trout liver cell line expressing cytochrome P450-dependent monooxygenase activity. Cell. Biol. Toxicol. 9: 279–294.
Li F., An H., Seymour T.A., Bradford C.S., Morrissey M.T., Bailey G.S. et al. 1998. Molecular cloning, sequence analysis and expression distribution of rainbow trout (Oncorhynchus mykiss) cystatin C. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 121: 135–143.
Lidgerding B.C., Phelps S.R. and Schill W.B. 1984. Fish cell lines: characterization by isozyme analysis. In Vitro 20: 167–171.
MacLeod R.A., Dirks W.G., Matsuo Y., Kaufmann M., Milch H. and Drexler H.G. 1999. Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int. J. Cancer 83: 555–563.
Markovic O. and Markovic N. 1998. Cell cross-contamination in cell cultures: the silent and neglected danger. In Vitro Cell. Dev. Biol. 34: 1–8.
Matsuo Y., Nishizaki C. and Drexler H.G. 1999. Efficient DNA fingerprinting method for the identification of cross-culture contamination of cell lines. Hum. Cell 12: 149–154.
McDonald G.J. 2001. Relatedness determination and detection of spawning time QTL in rainbow trout (Oncorhynchus mykiss), M.Sc., University of Guelph, Department of Zoology, Guelph, ON, Canada.
Morris D.B., Richard K.R. and Wright J.M. 1996. Microsatellites from rainbow trout (Oncorhynchus mykiss) and their use for genetic study of salmonids. Can. J. Fish Aquat. Sci. 53: 120–126.
Nelson-Rees W.A., Flandermeyer R.R. and Daniels D. 1980. T-1 cells are HeLa and not from normal human kidney origin. Science 209: 719–720.
Newton C.R. and Graham A. 1997. PCR: second edition. Springer-Verlag, New York.
O'Connell M., Danzmann R.G., Cornuet J.M., Wright J.M. and Ferguson M.M. 1997. Differentiation of rainbow trout populations in Lake Ontario and the evaluation of the stepwise mutation and infinite allele mutation models using microsatellite variability. Can. J. Fish Aquat. Sci. 54: 1391–1399.
O'Reilly P.T. and Wright J.M. 1995. The evolving technology of DNA fingerprinting and its application to fisheries and aquaculture. J. Fish Biol. 47: 29–55.
O'Reilly P.T., Hamilton L.C., McConnell S.K. and Wright J.M. 1996. Rapid analysis of genetic variation in Atlantic salmon (Salmo salar) by PCR multiplexing of dinucleotideand tetranucleotide microsatellites. Can. J. Fish Aquat. Sci. 53: 2292–2298.
Park L.K. and Moran P. 1994. Developments in molecular genetic techniques in fisheries. Rev. Fish Biol. Fisheries 4: 272–299.
Roy N.K., Maceda L. and Wirgin I. 2000. Isolation of microsatel-lites in striped bass Morone saxatilis(Teleostei) and their preliminary use in population identification. Mol. Ecol. 9: 827–829.
Sakamoto T. 1996. Study for the introduction of positional cloning method using DNA markers in fisheries science, PhD, Tokyo University of Fisheries.
Sakamoto T., Okamoto N. and Ikeda Y. 1994. Dinucleotide repeat polymorphism of rainbow trout, FGT 5. J. Anim. Sci. 72: 27–68.
Sanchez J.A., Clabby C., Ramos D., Blanco G., Flavin F., Vazquez E. et al. 1996. Protein and microsatellite single locus variability in Salmo salarL (Atlantic salmon). Heredity 77: 423–432.
Scribner K.T., Gust J.R. and Fields R.L. 1996. Isolation and characterization of novel microsatellite loci: Cross-species amplification and population genetics applications. Can. J. Fish Aquat. Sci. 53: 841–883.
Segner H. 1998. Fish cell lines as a tool in aquatic toxicology. In: Braunbeck T., Hinton D.E. and Streit B. (eds), Ecotoxicology. Birkhäuser, Basel, pp. 1–38.
Tom D.J., Lee L.E.J., Lew J. and Bols N.C. 2001. Induction of 7-ethoxyresorufin-o-deethylase activity by planar chlorinated hydrocarbons and polycyclic aromatic hydrocarbons in cell lines from the rainbow trout pituitary. Comp. Biochem. Physiol A. Mol. Integr. Physiol. 128: 185–198.
Wenburg J.K., Olsen J.B. and Bentzen P. 1996. Multiplexed systems of microsatellites for genetic analysis in coastal cutthroat trout (Oncorhynchus clarki clarki) and steelhead (Oncorhynchus mykiss). Mol. Mar. Biol. Biotechnol. 5: 273–283.
Wester W., Ellender R.D. and Brundage B. 1975. Characterization of lactic dehydrogenase isoenzymes from 6 fish cell lines. J. Miss. Acad Sci. 20: 47–47.
Wolf K. 1988. Fish Viruses and Fish Viral Diseases. Comstock Publishing Associates, Ithaca.
Wolf K. and Quimby M.C. 1962. Established eurythermic line of fish cells in vitro. Science 135: 1065–1066.
Yan Y., Ottenbreit M., Hukku B., Mally M., Chou S. and Kaplan J. 1996. DNA fingerpirnting of human cell lines using PCR amplification of fragment length polymorphisms. In Vitro Cell Dev. Biol. 32: 656–662.
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Perry, G., McDonald, G., Ferguson, M. et al. Characterization of rainbow trout cell lines using microsatellite DNA profiling. Cytotechnology 37, 143–151 (2001). https://doi.org/10.1023/A:1020516804173
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DOI: https://doi.org/10.1023/A:1020516804173