Abstract
The CCE/CC128 cell line, derived from fertilized eggs of theMediterranean fruit fly (Ceratitis capitata), was used toinvestigate whether insect cells in culture could developresistance to Malathion. After 20 cycles of pulse-chasetreatment (28 h exposure to 90 μg/ml of Malathion and 48 hrecovery in normal medium), a Mal 90 selected population wasobtained. DNA content analysis showed that the values were distributed between levels 2C and 4C and no accumulation ofcells in a specific phase of the cell cycle was observed.Furthermore, preliminary molecular analysis showed noamplification of the esterase gene in resistant cells.Cross-resistance of Mal 90 cells towards other insecticides wasassayed and found to be absent. Our data support the idea thatthe medfly cell line and, more generally, insect cell cultures,could represent a promising system to investigate insecticideresistance mechanisms.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baeza-Squiban A, Best-Belpomme M and Marano F (1988) Cytoxicity, accumulation, and metabolism of Deltamethrin, a pyrethroid insecticide, inDrosophila melanogaster cells. Pest Biochem Physiol 33: 201–212.
Cavalloro R (1981) Establishment of continuouslyin vitro growing cell lines ofMed-fly (Ceratitis capitata Wied.). Rev Can Biol 40: 181–186.
Field LM, Devonshire AL and Forde BG (1988) Molecular evidence that insecticide resistance in peach-potato aphids (Myzus persicae Sulz.) results from amplification of an esterase gene. Biochem J 251: 309–312.
Georghiou GP and Lagunes-Tejeda A (1991) The Occurrence of Resistance to Pesticides in Arthropods. An Index of Cases Reported Through 1989. F.A.O., Rome, Italy: 11–22.
Koren B, Yawetz A and Perry AS (1984) Biochemical properties characterizing the development of tolerance to Malathion inCeratitis capitata Wiedemann (Diptera: Tephritidae). J Econ Entomol 77: 864–867.
Miller SA, Dykes DD and Polesky HF (1989) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16: 1215–1216.
Mouchès C, Pasteur N, Bergé JB, Hyrien O, Raymond M, De Saint Vincent BR, De Silvestri M and Georghiouos GP (1986) Amplification of an esterase gene is responsible for insecticide resistance in a CaliforniaCulex mosquito. Science 233: 778–780.
Orphanidis PS, Kalmoukos B, Betzios B and Kapetanakis E (1980) Development of resistance inCeratitis capitata Wied. in laboratory under intermittent pressure of organophosphorous and chlorinated insecticides. Annales de l'Institute Phytopathologique Benaki (N.S.) 12: 198–207.
Rainaldi G, Pinto B, Piras A, Vatteroni L, Simi S and Citti L (1991) Reduction of proliferative heterogeneity of CHEF 18 Chinese hamster cell line during the progression toward tumorigenicity.In Vitro Cell Dev Biol 27/A: 949–952.
Yanangimoto Y, Sato K and Mitsuhashi J (1990) Differences in effects of rotenone on insect cell lines. Altern Anim Test Exp 1: 10–19.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rossi, E., Rainaldi, G. Induction of Malathion resistance in CCE/CC128 cell line of Mediterranean fruit fly (Ceratitis capitata (Wied.)) (Diptera: Tephritidae). Cytotechnology 34, 11–15 (2000). https://doi.org/10.1023/A:1008179521711
Issue Date:
DOI: https://doi.org/10.1023/A:1008179521711