Abstract
The infection of insect cells with baculovirus was described in a mathematical model as a part of the structured dynamic model describing whole animal cell metabolism. The model presented here is capable of simulating cell population dynamics, the concentrations of extracellular and intracellularviral components, and the heterologous product titers. The model describes the whole processes of viral infection and theeffect of the infection on the host cell metabolism. Dynamic simulation of the model in batch and fed-batch mode gave goodagreement between model predictions and experimental data. Optimum conditions for insect cell culture and viral infectionin batch and fed-batch culture were studied using the model.
Similar content being viewed by others
References
Barford JP, Phillips PJ and Harbour C (1992) Simulation of animal cell metabolism, Cytotechnology 10: 63–74.
Chan LCL (1998) Optimization of recombinant protein production in the baculovirus expression vector system using batch, fed-batch and perfusion culture. Ph.D. Thesis (Chemical Engineering), University of Queensland, Australia.
Fu PC and Barford JP (1994) Methods and strategies available for the process control and optimisation of monoclonal antibody production. Cytotechnology 14: 219–232.
Miller CK (1993) Baculovirus: high level expression in insect cells. Curr Opin Genet Dev 3: 97–101.
O'Reilly DR, Miller LK and Lucklow VA (1992) Baculovirus Expression Vectors: A Laboratory Manual. WH Freeman, New York.
Power JF, Reid S, Radford KM, Greenfield PF and Nielson LK (1994) Modeling and optimization of the baculovirus expression vector system in batch, suspension culture. Biotechnol Bioeng 44: 710–719.
Radford KM, Reid S and Greenfield PF (1997) Substrate limitation in the baculovirus expression vector system. Biotechnol Bioeng 56: 32–44.
Sanderson CS, Barford JP and Barton GW (1994) Modeling and optimization of cell cultures. IChemE Symp Series 137.
Sanderson CS (1997) Development and application of a structured model for animal cell metabolism. Ph.D. Thesis, University of Sydney, Australia.
Sanderson CS, Barford JP and Barton GW (1999a) A structured, dynamic model for animal cell culture system. Biochem Eng J 3: 203–211.
Sanderson CS, Jang JD, Barford JP and Barton GW (1999b) A structured, dynamic model for animal cell culture systems: Application to murine hybridoma. Biochem Eng J 3: 213–218.
Sanderson CS, Barford JP, Barton GW, Wong TKK and Reid S (1999c) A structured, dynamic model for animal cell culture: Application to baculovirus/insect cell systems. Biochem Eng J 3: 219–229.
Volkman LE, Goldsmith PA and Hess RT (1986) Alternate pathway of entry of buddedAutographa californica nuclear polyhedrosis virus: Fusion at the plasma membrane. Virology 148: 288–297.
Wong KTK, Nielsen LK, Greenfield PF and Reid S (1994) Relationship between oxygen uptake rate and time of infection of SF9 insect cells infected with recombinant baculovirus. Cytotechnology 15: 157–167.
Wong KTK, Peter CH, Greenfield PF, Reid S and Nielsen LK (1996) Low multiplicity infection of insect cells with a recombinant baculovirus: The cell yield concept. Biotechnol Bioeng 49: 659–666.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jang, J.D., Sanderson, C.S., Chan, L.C.L. et al. Structured modeling of recombinant protein production in batch and fed-batch culture of baculovirus-infected insect cells. Cytotechnology 34, 71–82 (2000). https://doi.org/10.1023/A:1008178029138
Issue Date:
DOI: https://doi.org/10.1023/A:1008178029138