Recognition by the European Community of élite landraces encourages farmers to grow these and earn more than is possible by growing modern varieties. However, farmers often exercise strong selection by collecting seed from a limited number of plants which best embody a few characters of prime interest. This, in the long run, may be responsible for considerable genetic erosion. In Southern Piedmont, North-West Italy, a local landrace of pepper (Capsicum annuum L.) known as ‘Cuneo’ is grown; it deserves particular attention because of its hardiness, late production and fruit quality. We used random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers to assess the effect, on genetic composition of a population, of seed sampling carried out using the selection criteria adopted by the farmer. After two reproductive cycles using selection, it was already possible to detect loss in genetic variation and a change in allele frequencies, while no significant effect was found after two cycles of random sampling. Over this period, farmer selection pressure led to disappearance of eight low-frequency alleles, while only three alleles were lost in randomly sampled populations. Our results stress the need to adopt appropriate techniques for seed sampling in order to avoid genetic erosion of local landrace ‘gene pools’.
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Bassam B.J., Caetano-Anolles G. and Gresshoff P.M. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytic Biochemistry 19: 680–683.
Bosland P.W. and Votava E.J. 2000. Peppers: Vegetable and Spice Capsicums J. Atherton and A. Rees eds, CABI Publishing, pp. 199.
Bussell J.D. 1999. The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Iso-toma petraea (Lobeliaceae). Molecular Ecology 8: 775–789.
Doyle J.J. and Doyle J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–14.
Fowler C., Hawtin G.C. and Hodgkin T. 1999. Forward. In: Brush S.B. (ed.), Genes in the Field: On-Farm Conservation of Crop Diversity. Lewis Publishers, Boca Raton, Florida, USA.
Franceschetti U. 1971. Natural crosspollination in pepper (Cap-sicum annuum L.). In: Proceedings of the Eucarpia Meeting on Genetics and Breeding of Capsicum, University of Turin, Italy, pp. 346–353.
Frankel O.H., Burdon J.J. and Peacock W.J. 1995. Landraces in transit-the treat perceived. Diversity 11: 14–15.
Gaudel M., Taberlet P. and Till-Bottraud I. 2000. Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment lenght polymorphism markers. Molecular Ecology 9: 1625–1637.
Gillet E.M. 1999. Sampling Strategies for Marker Analysis. Final Compendium of the Reserach Project “Development, optimization and validation of molecular tools for assessment of biodiversity in forest trees” European Union DGXII Biotechnol-ogy FW IV Reserach Programme “Molecular Tools for Biodiversity. (http: / /webdoc.sub.gwdg.de/ebook/y/1999/ whichmarker. htm).
Gregorious H.R. 1980. The probability of losing an allele when diploid genotypes are sampled. Biometrics 36: 643–652.
Hamrick J.L. and Godt M.J.W. 1996. Effects of life history traits on genetic diversity in plant species. Philosophical Transactions of the Royal Society of London, Series B 351: 1291–1298.
Harris S.A. 1995. Systematics and random amplified polymorphic DNA in the genus Leucaena (Leguminosae, Mimosoideae). Plant Systematics and Evolution 197: 195–208.
Hurme P. and Savolainen O. 1999. Comparison of homology and linkage of random amplified polymorphic DNA (RAPD) markers between individual trees of Scots pine (Pinus sylvestris L.). Molecular Ecology 8: 15–22.
IPGRI 1977. Strengthening the scientific basis of ‘in situ’ conservation of agricultural biodiversity on-farm. Options for data collecting and analysis. Proceedings of aWorkshop to develop tools and procedures for in situ conservation on-farm, August Rome, Italy, pp. 25–29.
Lanteri S., Di Leo I., Ledda L., Mameli M.G. and Portis E. 2001. RAPD variation within and among populations of globe artichoke cultivar 'spinoso sardo'. Plant Breeding 120: 243–246.
Lanteri S., Acquadro A., Quagliotti L. and Portis E. 2003. RAPD and AFLP assessment of genetic variation in a landrace of pepper (Capsicum annuum L.), grown in North-West Italy. Genet. Resour. Crop Evol. (in press).
LefebvreV., Palloix A., Caranta C. and Pochard E. 1995. Construc-tion of an intraspecific integrated linkage map of pepper using molecular markers and doubled-haploid progenies. Genome 38: 112–121.
Lefebvre V., Caranta C., Pflieger S., Moury B., Daubeze A.M., Blattes A. et al. 1997. Updated intraspecific maps of pepper. Capsicum and Eggplant Newsletter 16: 35–41.
Liu F., Zhang L. and Charlesworth D. 1998. Genetic diversity in Leavenworthia populations with different inbreeding levels Proceedings of the Royal Society of London, Series B. 265, pp. 293–301.
Maxted N., Guarino L., Myer L. and Chiwona E.A. 2002. Towards a methodology for on farm conservation of plant genetic re sources. Genet. Resour. Crop Evol. 49: 31–46.
Pickersgill B. 1977. Genetic resources and breeding of Capsicum spp. Euphytica 96: 129–245.
Piergiovanni A.R. and Laghetti G. 1999. The common bean land-races from Basilicata (Southern Italy): an example of integrated approach applied to genetic resources management. Genet. Re-sour. Crop Evol. 46: 47–52.
Steward C.N.J. and Porter D.M. 1995. RAPD profiling in biological conservation: an application to estimating clonal variation in rare and endangered Iliamna in Virginia. Biological Conservation 74: 132–142.
Tanksley S.D. 1984. High rates of cross-pollination in chile pepper. HortScience 19: 580–582.
Vos P., Hogers R., Bleeker M., Reijand M., van de Lee T., Hornes M. et al. 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407–4414.
Votava E.J., Gary P.N. and Bosland P.W. 2002. Genetic diversity and similarity revealed via molecular analysis among and within ' an in situ population and ex situ accessions of chiltepìn (Cap sicum annuum var. glabriusculum). Conservation Genetics 3: 123–129.
Waugh R., Bonar N., Baird E., Thomas B., Graner A., Hayes P. et al. 1997. Homology of AFLP products in three mapping popula tions of barley. Molecular and General Genetics 255: 311–321.
Welsh J. and McClelland M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18: 7213–7218.
Williams J., Kubelik A.R., Livak J.A., Rafalski J.A. and Tingey S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research 18: 6531–6535
Wood D. and Lenné J.M. 1997. The conservation of agrobiodiversi ty on farm: questioning the emerging paradigm. Biodiversity and Conservation 6: 109–129
Wu J., Krutovskii K. and Strauss S.H. 1999. Nuclear DNA diversity population differentiation and phylogenetic relationships in the California close-clone pines based on RAPD and allozyme markers. Genome 42: 893–908.
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Portis, E., Acquadro, A., Comino, C. et al. Effect of farmers' seed selection on genetic variation of a landrace population of pepper (Capsicum annuum L.), grown in North-West Italy. Genetic Resources and Crop Evolution 51, 581–590 (2004). https://doi.org/10.1023/B:GRES.0000024648.48164.c3
- Capsicum annuum
- In situ germplasm conservation