Elemental analysis in cultured cells, tobacco and grape, treated with aluminum
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We discussed the relationship between Al toxicity and the Al, Fe andB amount of element in tobacco and grape cells. Al and Fe were analyzed byneutron activation analysis and B was analyzed by prompt gamma-ray analysis.Callose content was also measured as an indicator of cell damage induced Altoxicity. When tobacco cells were incubated in 1 mM and 300 µM Al solution,the pattern of callose formation was much similar to that of Fe accumulationthan that of Al accumulation in tobacco cells, suggesting that the increaseof Fe content induced toxic effect along with Al incorporated into the cells.However, this tendency was not observed in grape cells. Boron content didnot show any relation to those of Al or Fe throughout the Al treatment inboth tobacco and grape cells.
KeywordsAluminum Physical Chemistry Toxicity Inorganic Chemistry Boron
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