Journal of Radioanalytical and Nuclear Chemistry

, Volume 249, Issue 2, pp 343–348 | Cite as

PIXE application for multi-element analysis of marine micro-algae and measurement of the concentration factor of zinc

  • Y. IwataEmail author


Marine micro-algae (Nannochloropsis sp., Phaeodactylum sp., etc.) wereobtained from the Pacific Ocean off Iwate Prefecture, Japan, and culturedpurely in nutritive seawater, PES and artificial seawater, ASP7 as a culturesolution. The algae in 5 ml of the culture solution was collected on a polycarbonatefilm (pore size : 1.0 µm) by suction filtration. The algae on the filmwas subjected to PIXE analysis. A 2.9 MeV proton beam bombarded it at 50 nAfor 15 minutes. Induced X-ray was measured by Si(Li) detector. Concentrationsof Na, Mg, Al, Si, P, S, Cl, K, Ca, Cr, Mn, Fe, Cu, Zn and Sr were determinedsimultaneously. PIXE can used for multi-element analysis of a sample below1 mg, so the culture size for algae was 10–250 ml and every apparatuswas small and of low cost. Nannochloropsis sp. was given a different culturesolution (PES) including Zn2+ from 0.050 to 1.0 mg/l and incubatedat 22 °C, 2000 lux for 10 hours. Zn in Nannochloropsis sp. increased inproportion to the Zn2+ concentration in the culture solution. Theconcentration factor was given as 1600 500 (dry weight base). It was shownthat PIXE was a powerful tool for multi-element analysis of micro organismsand the measurement of bioaccumulation.


Pacific Ocean Bioaccumulation Weight Base Proton Beam Concentration Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    H. J. Bowen, Environmental Chemistry of the Elements, Academic Press, 1979.Google Scholar
  2. 2.
    R. Elaler, Trace Metals Concentrations in Marine Organisms, Pergamon Press, 1982.Google Scholar
  3. 3.
    N. Suzuki,Y. Iwata, Appl. Organomet. Chem., 4 (1990) 287.CrossRefGoogle Scholar
  4. 4.
    Y. Iwata,H. Naitoh,N. Suzuki, J. Radioanal. Nucl. Chem., 159 (1992) 121.CrossRefGoogle Scholar
  5. 5.
    N. Suzuki,Y. Iwata,H. Imura, Intern. J. Environ. Anal. Chem., 30 (1987) 289.CrossRefGoogle Scholar
  6. 6.
    Y. Iwata,H. Imura,N. Suzuki, J. Radioanal. Nucl. Chem., 172 (1993) 305.CrossRefGoogle Scholar
  7. 7.
    Y. Iwata,H. Imura,N. Suzuki, Anal. Chim. Acta, 219 (1989) 97.CrossRefGoogle Scholar
  8. 8.
    Y. Iwata,T. Korenaga,N. Suzuki, Intern. J. PIXE, 3 (1991) 271.CrossRefGoogle Scholar
  9. 9.
    Y. Iwata,N. Suzuki, J. Radioanal. Nucl. Chem., 233 (1998) 121.CrossRefGoogle Scholar
  10. 10.
    S. A. E. Johansson,T. B. Johansson, Nucl. Instr. Meth., 137 (1976) 473CrossRefGoogle Scholar
  11. 11.
    Y. Iwata,T. Fujiwara,N. Suzuki, Intern. J. PIXE, 2 (1992) 381.CrossRefGoogle Scholar
  12. 12.
    J. D. Macarthur,G. R. Palmer,K. Budd,W. E. Hekman,B. E. Nichol,J. R. Casey, Nucl. Instr. Meth., B10/11, (1985) 653Google Scholar
  13. 13.
    L. Provasoli, Proc. of US-Japan Conf. on Cultures and Collection of Algae, A. Watanabe,A. Hattori (Eds), Japan Society of Plant Physiology, 1968, p. 63.Google Scholar
  14. 14.
    K. Sera,T. Yanagisawa,H. Tsunoda,S. Futatsugawa,S. Hatakeyama,Y. Saitou,S. Suzuki,H. Orihara, Intern. J. PIXE, 3 (1993) 325.CrossRefGoogle Scholar
  15. 15.
    K. Sera,S. Futatsugawa, Nucl. Instr. Meth., B 109/110 (1996) 99.CrossRefGoogle Scholar
  16. 16.
    F. J. Millero, Chemical Oceanography, 2nd ed., CRC Press, 1996, p. 98.Google Scholar
  17. 17.
    C. F. Baes, Jr.,R. E. Mesmer, The Hydrolysis of Cations, John Wiley & Sons, 1976, p. 294.Google Scholar
  18. 18.
    Y. Iwata,H. Naitoh,N. Suzuki, Res. Rep. Lab. Nucl. Sci., Tohoku Univ., 24 (1991) 255 (in Japanese).Google Scholar

Copyright information

© Kluwer Academic Publishers/Akadémiai Kiadó 2001

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of Education and Human StudiesAkita UniversityAkitaJapan

Personalised recommendations