Pharmacy World and Science

, Volume 19, Issue 6, pp 283–286 | Cite as

A method for measuring nitric oxide radical scavenging activity. Scavenging properties of sulfur–containing compounds.

  • Martijn F. Vriesman
  • Guido R.M.M. Haenen
  • Gerrit–Jan Westerveld
  • Jos B.G. Paquay
  • Hans–Peter Voss
  • Aalt Bast


A new method for the quantification of the nitric oxide (•NO) scavenging activity of compounds in aqueous solutions is described using an amperometric •NO sensor. After correction for the spontaneous degradation of •NO, second–order rate kinetics of the scavenging reaction are observe. The rate constant for hemoglobin found with this method is comparable with that found with an established spectrophotometric method. To demonstrate the capability of the method, several sulfur–containing compounds were tested (GSH, GSSG, S–methyl glutathione, N–acetyl cysteine, lipoic acid and dihydrolipoic acid). Of these compounds, only those that contained a thiol group displayed a considerable •NO scavenging activity.

Nitric oxide radical •NO, scavenging Thiol S–nitrosothiol (electrochemical) NO sensing 


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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Martijn F. Vriesman
    • 1
  • Guido R.M.M. Haenen
    • 1
  • Gerrit–Jan Westerveld
    • 1
  • Jos B.G. Paquay
    • 1
  • Hans–Peter Voss
    • 1
  • Aalt Bast
    • 1
  1. 1.Leiden/Amsterdam Center for Drug Research, Division of Molecular Pharmacology, Department of Pharmacochemistry, Faculty of ChemistryVrije UniversiteitAmsterdamThe Netherlands

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