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Action of Juvenile Hormone and Ecdysone in the Metamorphic Endocrine Centres

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Abstract

The relationship between corpus allatum (CA) and prothoracic gland (PG) activities has been examined during postfeeding larval and prepupal stages of en-silkworm, Philosamia ricini (Dru.). Using the isolated larval abdomen technique, the critical period of ecdysone (MH) was determined. It was 114 hr immediately after the beginning of cocoon spinning at 20 ± 2°C. To study the CA activity a histometric method was used. CA volumes suddenly increased at the prepupal stage. From these data a clear positive correlation between CA and PG activities was recognized.

Brainless Ph. ricini pupae, a highly reliable bioassay organism for detecting prothoracicotropic (PTTH)-like effects, were produced. The debraination of Ph. ricini larvae after the critical period of PTTH, produced brainless pupae. This bioassay organism was used for studying the action of juvenile hormone (JH) and MH in the metamorphic endocrine centres during the absence of the brain.

Injection of 5 μg altozar (JHA) into debrained Ph. ricini larvae accelerated pupation (12.24 ± 0.36 instead of 16.51 ± 0.19 days). Moreover the produced brainless pupae moulted to second brainless pupae. The injection of 5 μg JHA into brainless pupae or isolated larval abdominae of Ph. ricini did not show any effects. Brainless Ph. ricini moths were obtained after injecting the brainless pupae with MH (10 μg-40 μg/p). The injection of high concentrations of MH (≥ 70 μg/p) into brainless pupae activated the CA and produced pupal-adult intermediates. The present data reveal that the PTTH at low concentrations, permits the action of a JHA in the PG. Also it shows that the MH at high levels activates the CA.

Résumé

La relation entre les activités de corpus allatum (CA) et celles de la glande prothoracique (PG) a été étudiée durant les stades larvaires (la période post nutritive larvaire) ainsi que le stade prepupal du vers à soie de ricin Philosamia ricini (Dru). En utilisant la technique d’abdomaines larvaires isolés, il a été possible de l’ecdysone (MH), comme étant de 114 hrs immédiatement après le commençement de la filature du coccon á la température de 20 ± 2°C le volume de CA a augmenté soudainement au stade previrginal.

De ces derniers résult↦s l’on peut déduire une claire correlation des activités de CA et PG.

Il a été possible de produire des pupes du vers a soie de Ricin Ph. ricini sans cerveau. Ces dernières représentent un organisme pour éssais biologiques auquels on peut faire hautement confiance, pour la détection de l’activité de l’hormone qui influe sur la glande prothoracique (PTTH). Les larves dont on a enlevé le cerveau après la periode critique de secretion de PTTH, ont produit des pupes sans cerveau. Cet organisme de bioéssais a été utilisé pour l’étude de l’action de l’hormone juvenile (JH) et MH dans les centres endocriniens métamorphiques durant l’abscence du cerveau.

L’injection de 5μg d’Altozar (JHA) a des larves decervelés (dont on a enlevé le cerveau) de Ph. ricini, a accéléré la transformation en pupe (12.24 ± 0.36 au lieu 16.51 ± 0.19 jours). Et qui plus est la production de pupe sans cerveau ont mue en seconde pupe sans cerveau. L’injection de 5 μg JHA á des pupes sans cerveau ou á des abdomaines larvaires isolés de Ph. ricini, n’a donné aucun résultat. Après l’infection des pupes décervelés avec MH (10 μg–40 μg/p) des papillons de Ph. ricini dans cerveau ont émergés. L’infection de hautes concentrations de (MH ≥ 100 μg/p) á des pupes sans cerveau, a activé le CA et a produit des individus intermédiaires pupe-adultes, les présentes résult↦s ont révéle que PTTH a de basses concentrations a permis l’action de JHA sur la glande prothoracique PG. Il est aussi évident que le MH a de hautes concentrations active le CA.

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  1. Department of Plant Protection, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt

    Mamdouh H. Idriss

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Idriss, M.H. Action of Juvenile Hormone and Ecdysone in the Metamorphic Endocrine Centres. Int J Trop Insect Sci 11, 159–166 (1990). https://doi.org/10.1017/S1742758400010523

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