International Journal of Tropical Insect Science

, Volume 34, Issue 3, pp 190–196 | Cite as

Susceptibility of developmental stages of Tetranychus urticae (Acari: Tetranychidae) to infection by Beauveria bassiana and Metarhizium anisopliae (Hypocreales: Clavicipitaceae)

  • David M. Bugeme
  • Markus Knapp
  • Hamadi I. Boga
  • Sunday Ekesi
  • Nguya K. ManianiaEmail author
Research Paper


In the present study, the susceptibility of different developmental stages (eggs, larvae, protonymphs, deutonymphs and adults) of Tetranychus urticae Koch to the entomopathogenic fungi Beauveria bassiana (Balsamo-Crivelli) Vuillemin and Metarhizium anisopliae (Metchnikoff) Sorokin was evaluated in the laboratory. At each developmental stage, four concentrations (3 × 105, 1 × 106, 3 × 106 and 1 × 107 conidia/ml) of both fungi were sprayed. Egg viability and motile-stage mortality varied among the fungal isolates and conidial concentrations. The highest conidial concentration (1 × 107 conidia/ml) significantly reduced the viability of eggs and increased the mortality of motile stages. However, deutonymphs and adults were more susceptible to fungal infection than larvae and protonymphs. The lethal concentration values to 50% (LC50) and 90% mortality (LC90) varied with the fungal isolates and developmental stages. The results show the prospects of B. bassiana and M. anisopliae for the control of T. urticae.

Key words

Beauveria bassiana Metarhizium anisopliae developmental stages fungal infection Tetranychus urticae susceptibility 


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Copyright information

© ICIPE 2014

Authors and Affiliations

  • David M. Bugeme
    • 1
    • 2
  • Markus Knapp
    • 1
  • Hamadi I. Boga
    • 2
  • Sunday Ekesi
    • 1
  • Nguya K. Maniania
    • 1
    Email author
  1. 1.International Centre of Insect Physiology and Ecology (icipe)NairobiKenya
  2. 2.Faculty of SciencesJomo Kenyatta University of Agriculture and Technology (JKUAT)NairobiKenya

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