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Use of the entomopathogenic fungi Metarhizium anisopliae, Cordyceps bassiana and Isaria fumosorosea to control Diaphorina citri (Hemiptera: Psyllidae) in Persian lime under field conditions

  • Roberto Lezama-Gutiérrez
  • Jaime Molina-OchoaEmail author
  • Omar Chávez-Flores
  • César Andrés Ángel-Sahagún
  • Steven R. Skoda
  • Gerardo Reyes-Martínez
  • Marisela Barba-Reynoso
  • Oscar Rebolledo-Domínguez
  • Graciela M. L. Ruíz-Aguilar
  • John E. Foster
Research Paper

Abstract

The Asian citrus psyllid Diaphorina citri Kuwayama is a destructive insect pest in citriculture, because it is an efficient vector of the proteobacteria ‘Candidatus Liberibacter asiaticus’ (Las), ‘Ca. L. africanus’ (Laf) and ‘Ca. L. americanus’ (Lam). These bacteria cause the ‘huanglongbing’ disease or ‘greening’ or ‘yellow dragon’ disease. The disease kills the plant and reduces fruit production. This insect pest is susceptible to entomopathogenic fungi, and we report the use of different strains of Metarhizium anisopliae, Cordyceps bassiana and Isaria fumosorosea against the nymphs and adults of D. citri under field conditions. The fungi were applied four times using a concentration of 2 × 1013 conidia/ha with a time interval of 15 days between applications. The percentage of control of Cb 108, Ma 65, Ma 14 and Ifr 4 was 60, 50, 40 and 35% in nymphs, and 50, 50, 42 and 22% in adults, respectively. Metarhizium anisopliae, C. bassiana and I. fumosorosea applied on Persian lime groves are more effective in reducing higher density of nymphs than adults of D. citri.

Key words

Diaphorina citri huanglongbing Persian lime Mexican lime biological control 

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Copyright information

© ICIPE 2012

Authors and Affiliations

  • Roberto Lezama-Gutiérrez
    • 1
  • Jaime Molina-Ochoa
    • 2
    • 3
    Email author
  • Omar Chávez-Flores
    • 1
  • César Andrés Ángel-Sahagún
    • 4
  • Steven R. Skoda
    • 5
  • Gerardo Reyes-Martínez
    • 6
  • Marisela Barba-Reynoso
    • 6
  • Oscar Rebolledo-Domínguez
    • 1
  • Graciela M. L. Ruíz-Aguilar
    • 2
  • John E. Foster
    • 3
  1. 1.Facultad de Ciencias Biológicas y AgropecuariasUniversidad de ColimaTecománMexico
  2. 2.División Ciencias de la Salud e Ingenierías, Departamento AgroindustrialUniversidad de Guanajuato, Campus Celaya-SalvatierraSalvatierra, GuanajuatoMexico
  3. 3.Department of Entomology, Insect Genetics LaboratoryUniversity of Nebraska-LincolnLincolnUSA
  4. 4.División de Ciencias de la Vida, Departamento de Agronomía, Campus Irapuato-SalamancaUniversidad de GuanajuatoIrapuato, GuanajuatoMexico
  5. 5.USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research LaboratoryKerrvilleUSA
  6. 6.Consejo Estatal de Productores de Limón (COEPLIM)TecománMexico

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