A study on phlebotomine sandflies (Diptera: Phlebotomidae) in Dickwella, southern Sri Lanka, an endemic focus for cutaneous leishmaniasis

  • W. T. S. Dammini PremachandraEmail author
  • D. P. C. Senarath
  • M. P. K. S. K. de Silva
  • B. S. Lalanthika Peiris
Research Paper


Cutaneous leishmaniasis (CL) has become an important health problem in Sri Lanka. Dickwella Divisional Secretariat Division (DSD) located in southern Sri Lanka has been identified as a highly endemic focus for CL. However, to date, the potential sandfly vectors of the Leishmania parasite causing CL have not been elucidated in this focus. This study aimed to determine the species composition and population dynamics of sandflies prevalent at three sampling stations representing different ecological conditions in two high-risk areas in Dickwella DSD, over a period of 27 consecutive weeks. Resting sandflies (i.e. indoor collection) were collected from the lavatories of human households using Castro aspirators at weekly intervals. In addition, cattle-baited traps were deployed at bi-weekly intervals to capture foraging sandflies (outdoor collection). Sergentomyia zeylanica Annandale was found to be the only sandfly species and hence was considered as the potential vector of the Leishmania parasite that causes CL. As a whole, 84 and 96% of the sampling occasions were positive for resting and foraging sandflies, respectively. Seventy-six per cent of the lavatories sampled contained sandflies regardless of the sampling station. Altogether, 34,481 sandflies were captured from lavatories, whereas 691 were collected in cattle-baited traps. The ecological habitat, which comprised dense vegetation and a stagnant water body, seemed to be highly productive for this sandfly species. However, dryness and heavy rains negatively affected its population density. The sex ratio of the sandflies was male-biased at 1:2 (females:males). The findings of this study suggested that the management strategies of S. zeylanica could be targeted on lavatories especially after moderate rains.

Key words

density Dickwella lavatories prevalence Sergentomyia zeylanica 


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  1. Alexander B. (2000) Sampling methods for phlebotomine sandflies. Medical and Veterinary Entomology 14, 109–122.CrossRefGoogle Scholar
  2. Athukorale D. N., Seneviratne J. K., Ihalamulla R. L. and Premaratne U. N. (1992) Locally acquired leishmaniasis in Sri Lanka. Journal of Tropical Medicine and Hygiene 95, 432–433.PubMedGoogle Scholar
  3. Chaniotis B. N. (1967) The biology of California Phlebotomus under laboratory conditions. Journal of Medical Entomology 4, 221–233.CrossRefGoogle Scholar
  4. Chaniotis B. N. and Correa M. A. (1974) Comparative flying and biting activity of Panamanian phlebotomine sandflies in mature forest and adjacent open space. Journal of Medical Entomology 8, 369–375.CrossRefGoogle Scholar
  5. Chaniotis B. N., James M. N., Correa M. A., Tesh R. B. and Johnson K. M. (1971) Daily and seasonal man-biting activity of Phlebotomus sandflies in Panama. Journal of Medical Entomology 8, 415–420.CrossRefGoogle Scholar
  6. Chelbi I., Kaabi B., Béjaoui M., Derbali M. and Zhioua E. (2009) Spatial correlation between Phlebotomus papatasi Scopoli (Diptera: Psychodidae) and incidence of zoonotic cutaneous leishmaniasis. Journal of Medical Entomology 46, 400–402.CrossRefGoogle Scholar
  7. Choi C. M. and Lerner E. A. (2001) Leishmaniasis as an emerging infection. Journal of Investigative Dermatology Symposium Proceedings 6, 175–182.CrossRefGoogle Scholar
  8. Desjeux P. (1996) Leishmaniasis: public health aspects and control. Clinical Dermatology 14, 417–423.CrossRefGoogle Scholar
  9. Guernaoui S. and Boumezzough A. (2009) Habitat preferences of phlebotomine sand flies (Diptera: Psychodidae) in southwestern Morocco. Journal of Medical Entomology 46, 1187–1194.CrossRefGoogle Scholar
  10. Guernaoui S., Boumezzough A., Pesson B. and Pichon G. (2005) Entomological investigations in Chichaoua: an emerging epidemic focus of cutaneous leishmaniasis in Morocco. Journal of Medical Entomology 42, 697–701.CrossRefGoogle Scholar
  11. Johnson P. T. and Hertig M. (1961) The rearing of Phlebotomus sandflies. II. Development and behavior of Panamanian sandflies in laboratory culture. Annals of the Entomological Society of America 54, 764–776.CrossRefGoogle Scholar
  12. Kalra N. L. and Bang Y. H. (1988) Manual on Entomology in Visceral Leishmaniasis. World Health Organization, Regional Office for South-East Asia, New Delhi.Google Scholar
  13. Karunaweera N. D., Pratlong F., Siriwardena H. V., Ihalamulla R. L. and Dedet J. P. (2003) Sri Lankan cutaneous leishmaniasis is caused by Leishmania donovani zymodeme MON-37. Transactions of the Royal Society of Tropical Medicine and Hygiene 97, 380–381.CrossRefGoogle Scholar
  14. Karunaweera N., Abeysinghe R., Porter C. and Dotson E. M. (2009) Study on sandfly population in a cutaneous leishmaniasis focus in Sri Lanka. 4th World Congress on Leishmaniasis, Lucknow, India. Abstract no. 384.Google Scholar
  15. Lane R. P., Pile M. M. and Amerasinghe F. P. (1990) Anthropophagy and aggregation behaviour of the sandfly Phlebotomus argentipes in Sri Lanka. Medical and Veterinary Entomology 4, 79–88.CrossRefGoogle Scholar
  16. Lewis D. J. (1967) The phlebotomine sand flies of west Pakistan (Diptera: Psychodidae). Bulletin of British Museum (Natural History) Entomology 19, 1–57.CrossRefGoogle Scholar
  17. Lewis D. J. and Killick-Kendrick R. (1973) Some phlebotomid sand flies and other Diptera of Malaysia and Sri Lanka. Transactions of the Royal Society of Tropical Medicine and Hygiene 67, 4–5.CrossRefGoogle Scholar
  18. Morrison A. C. (1993) Dispersal of the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) at an endemic focus of visceral leishmaniasis in Colombia. Journal of Medical Entomology 30, 427–435.CrossRefGoogle Scholar
  19. Naotunne T. D., Rajakulendran S., Abewickreme W., Kulasiri C. D., Perera J., Premaratne U. N., Attygalle D. and Mendis K. N. (1990) Cutaneous leishmaniasis in Sri Lanka: an imported disease linked to the Middle East and African employment boom. Tropical and Geographical Medicine 42, 72–74.PubMedGoogle Scholar
  20. Ozbel Y., Sanjoba C., Alten B., Asada M., Depaquit J., Matsumoto Y., Demir S., Siyambalagoda R. R. M. L. R., Rajapakse R. P. V. J. and Matsumoto Y. (2011) Distribution and ecological aspects of sand fly (Diptera: Psychodidae) species in Sri Lanka. Journal of Vector Ecology 36 (suppl. s1), S77–86.CrossRefGoogle Scholar
  21. Perfiliew P. P. (1968) Fauna of the USSR Diptera: Phlebotomidae (sandflies). Fauna of the USSR, vol. 3, no. 2. Israel Program for Scientific Translations, Jerusalem, Israel.Google Scholar
  22. Pessoa S. B. and Barretto M. P. (1948) Leishmamiose Tegumentar Americana. Imprensa Nacional, Rio de Janeiro.Google Scholar
  23. Quate W. L. (1962) Psychodidae (Diptera) at the zoological survey of India. Proceedings of the Hawaiian Entomological Society 18, 155–188.Google Scholar
  24. Rajapaksa U. S., Ihalamulla R. L., Udagedera C. and Karunaweera N. D. (2007) Cutaneous leishmaniasis in southern Sri Lanka. Transactions of the Royal Society of Tropical Medicine and Hygiene 101, 799–803.CrossRefGoogle Scholar
  25. SAS Institute (1999) SAS/STAT User’s Guide. SAS Institute, Cary NC.Google Scholar
  26. Seneviratne J. K. K., Siriwardena S., Ratnatunga N. and Wijesundera M. de S. (1995) Locally acquired cutaneous leishmaniasis from central Sri Lanka: a case report. Kandy Medical Journal 4, 54–56.Google Scholar
  27. Sherlock I. A. and Sherlock V. A. (1959) Criação e biologia em laboratório do Phlebotomus longipalpis (Lutz & Neiva, 1912). Revista Brasileira de Biologia 19, 229–250.Google Scholar
  28. Singh R., Lal S. and Saxena K. V. (2008) Breeding ecology of visceral leishmaniasis vector sand fly in Bihar state of India. Acta Tropica 107, 117–120.CrossRefGoogle Scholar
  29. Siriwardena H. V. Y. D., Udagedara C. U. and Karunaweera N. D. (2003) Clinical features, risk factors and efficacy of cryotherapy in cutaneous leishmaniasis in Sri Lanka. Ceylon Medical Journal 48, 10–12.CrossRefGoogle Scholar
  30. Theodor O. (1938) On sandflies (Phlebotomus) from Ceylon, Siam and Malaya. Indian Journal of Medical Research 26, 261–269.Google Scholar
  31. Toprak S. and Özer N. (2007) Distribution of sand fly (Diptera: Psychodidae) species and efficiency of capturing methods in Sanliurfa Province, Turkey. Journal of Medical Entomology 44, 23–28.CrossRefGoogle Scholar

Copyright information

© ICIPE 2012

Authors and Affiliations

  • W. T. S. Dammini Premachandra
    • 1
    Email author
  • D. P. C. Senarath
    • 1
  • M. P. K. S. K. de Silva
    • 1
  • B. S. Lalanthika Peiris
    • 2
  1. 1.Department of ZoologyUniversity of RuhunaMataraSri Lanka
  2. 2.Anti-Malaria CampaignHambanthotaSri Lanka

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