International Journal of Tropical Insect Science

, Volume 31, Issue 4, pp 262–268 | Cite as

Dose transfer of an oil-based formulation of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) sprays to cotton bollworm in an arena trial

  • O. K. Douro KpindouEmail author
  • D. A. Djegui
  • I. A. Glitho
  • M. Tamò


A cotton field was used to set up an arena trial for investigating the dose transfer of oil-based ultra-low-volume sprays of Metarhizium anisopliae (Metchnikoff) Sorokin to the cotton bollworm Helicoverpa armigera (Hübner). Two doses of conidia (75 and 50g/ha, being 3.75 × 10 and 2.5 × 1012 conidia/ha, respectively) formulated in 2 litres (70:30, kerosene-peanut oil) were applied using a Berthoud ‘Micro Ulva’ to three groups of larvae of cotton bollworms positioned in three rows downwind of a single spray line. Both the distribution of the droplets and the effect of direct contact with spray droplets were quantified. The mean number of droplets decreased from 416 (± 60) droplets per cm2 (line 1;0.4m) to 45 (±36) droplets per cm2 (line 3; 5 m). To achieve 50% mortality, >10 and >13 days were necessary for 75 and 50g/ha, respectively. From day 15, the difference in mortality rates was significant between the two treatments. At line 3 (5 m), at 12 days after application, the mortality rate reached 52% for 75g/ha and 47% for 50g/ha. Fhe results are discussed in view of optimizing the application of the biopesticides.

Key words

Helicoverpa armigera Metarhizium anisopliae biopesticide dose transfer cotton field ultra-low-volume application 


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Copyright information

© ICIPE 2011

Authors and Affiliations

  • O. K. Douro Kpindou
    • 1
    Email author
  • D. A. Djegui
    • 1
  • I. A. Glitho
    • 2
  • M. Tamò
    • 1
  1. 1.International Institute of Tropical AgricultureCotonouBenin
  2. 2.Laboratoire d’Entomologie Appliquée, Faculté des SciencesUniversité de LoméLoméTogo

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