Dose transfer of an oil-based formulation of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) sprays to cotton bollworm in an arena trial
A cotton field was used to set up an arena trial for investigating the dose transfer of oil-based ultra-low-volume sprays of Metarhizium anisopliae (Metchnikoff) Sorokin to the cotton bollworm Helicoverpa armigera (Hübner). Two doses of conidia (75 and 50g/ha, being 3.75 × 10 and 2.5 × 1012 conidia/ha, respectively) formulated in 2 litres (70:30, kerosene-peanut oil) were applied using a Berthoud ‘Micro Ulva’ to three groups of larvae of cotton bollworms positioned in three rows downwind of a single spray line. Both the distribution of the droplets and the effect of direct contact with spray droplets were quantified. The mean number of droplets decreased from 416 (± 60) droplets per cm2 (line 1;0.4m) to 45 (±36) droplets per cm2 (line 3; 5 m). To achieve 50% mortality, >10 and >13 days were necessary for 75 and 50g/ha, respectively. From day 15, the difference in mortality rates was significant between the two treatments. At line 3 (5 m), at 12 days after application, the mortality rate reached 52% for 75g/ha and 47% for 50g/ha. Fhe results are discussed in view of optimizing the application of the biopesticides.
Key wordsHelicoverpa armigera Metarhizium anisopliae biopesticide dose transfer cotton field ultra-low-volume application
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- Bateman R. P., Godonou I., Douro Kpindou O. K., Lomer C. J. and Paraiso A. (1992) Development of a novel “field bioassay” technique for assessing mycopesticide U.L.V. formulations, pp. 255–262. In Biological Control of Locusts and Grasshoppers (edited by C. J. Lomer and C. Prior). CAB International, Wallingford.Google Scholar
- Bateman R. P., Price R. F., Müller E. J. and Brown H. D. (1994) Controlling brown locust hopper bands in South Africa with a myco-insecticide spray, pp. 609–616. Proceedings of Brighton Crop Protection Conference — Pest and Diseases, 16–19 November 1994, Brighton, UK.Google Scholar
- Bateman R. P. and Thomas M. (1996) Pathogen application against locusts and grasshoppers: insecticide or biological control? Antenna 20, 10–15.Google Scholar
- Douro Kpindou O. K., Djegui D. A., Glitho I. A. and Tamò M. (2010) Réponse des stades larvaires de Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) à différentes doses d’application des champignons entomopathogènes Metarhizium anisopliae et Beauveria bassiana. Biotechnologie Agronomie et Environnement.Google Scholar
- Hewitt H. G., Caseley J., Copping L. G., Grayson B. T. and Tyson D. (eds) (1994) Comparing glasshouse and field pesticide performance 11, British Crop Protection Council Monograph No. 59}. 323 pp.Google Scholar
- Kulkarni S. A., Ghormade V., Kulkarni G., Kapoor M., Chavan S. B., Rajendran A., Patil S. K., Shouche Y. and Deshpande M. V. (2008) Comparison of Metarhizium isolates for biocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) in chickpea. Biocontrol Science and Technology 18, 809–828.CrossRefGoogle Scholar
- Kumar V. and Chowdhry P. N. (2004) Virulence of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against tomato fruit borer, Helicoverpa armigera. Indian Phytopathological Society 54, 208–212.Google Scholar
- Langewald J., Thomas M. B., Douro Kpindou O. K. and Lomer C. J. (1997) Use of Metarhizium flavoviride for control of Zonocerus variegatus: a model, linking dispersal and secondary infection from the spray residue with mortality in caged field samples. Entomologia Experimentalis et Applicata 82, 1–8.CrossRefGoogle Scholar
- Matthews G. A. (1992) Pesticide Application Methods, 2nd edn. Longmans, Harlow. 405 pp.Google Scholar
- SAS (2002–2008). SAS® 9.2, Copyright © 2002–2008 by SAS Institute Inc., Cary, NC, USA.Google Scholar
- Staniland L. N. (1959) Fluorescent tracer for the study of spray and dust deposits. Journal of Agricultural Engineering Research 4, 42–81.Google Scholar
- Teakle R. E. and Jensen J. M. (1985) Heliothis punctigera, pp. 313–322. In Handbook of Insect Rearing (edited by P. Singh and R. F. Moore). Elsevier, Amsterdam.Google Scholar