Interaction of Dicladispa armigera (Coleoptera: Chrysomelidae) haemocytes with Beauveria bassiana

  • Moushumi Phukan
  • L. K. HazarikaEmail author
  • Madhumita Barooah
  • K. C. Puzari
  • S. Kalita


Morphology of haemocytes of healthy and Beauveria bassiana (Bals.) Vuill.-infected Dicladispa armigera (Olivier) adults was examined in a phase contrast microscope to characterize them into different types. The total haemocyte count (THC) and the differential haemocyte count (DHC) were also estimated. Four types of haemocytes were identified: prohaemocyte, plasmatocyte, granulocyte and spherulocyte. In the healthy adults, the THC varied between 5055 and 5950/mm3, out of which 85% were composed of granulocytes and plasmatocytes. As B. bassiana infection time increased, THC decreased and was associated with a significant concomitant increase in granulocytes and a decrease in prohaemocytes. Granulocytes reacted in various ways, including disintegration of the plasma membrane, formation of fine pseudopod-like cytoplasmic extensions and finally clumping of cells. Granulocytes and plasmatocytes were the predominant haemocytes involved in all the cell-mediated defence reactions.

Key words

Dicladispa armigera Beauveria bassiana cellular immunity encapsulation 


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Copyright information

© ICIPE 2008

Authors and Affiliations

  • Moushumi Phukan
    • 1
  • L. K. Hazarika
    • 1
    Email author
  • Madhumita Barooah
    • 2
  • K. C. Puzari
    • 3
  • S. Kalita
    • 1
  1. 1.Department of EntomologyAssam Agricultural UniversityJorhatIndia
  2. 2.Department of Agricultural BiotechnologyAssam Agricultural UniversityJorhatIndia
  3. 3.Mycology Research SectionAssam Agricultural UniversityJorhatIndia

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