Inherited Sterility in Different Strains of Ephestia Cautella (Lepidoptera: Pyralidae)

  • Ayad A. Al-Taweel
  • Mohammed S. H. Ahmed
  • Sarab S. Kadhum
  • Asaad A. Hameed
  • Majdolin J. Nasser
Research Article


Partial sterility induced by gamma irradiation of Ephestia cautella adult males was studied in five laboratory strains (A, B, C, D and E) which exhibit conspicuous genetic variation in the adult fore wing pigmentation. Four of these strains had been reared in the laboratory for more than 80 generations, while the fifth strain has been reared for only 10 generations. When males irradiated with 0.2 kGy were crossed with untreated females, the per cent egg hatch was reduced significantly as compared to the unirradiated control crosses. Strain C showed the highest reduction in the per cent egg hatch followed by a gradual decrease in radiation sensitivity in strains A, E, D and B, respectively. Fecundity and mating frequency appeared not to be affected but the sex ratios were clearly distorted (about two males to one female) in all strains. F1 progeny of all strains were either sterile when mated together (per cent egg hatch=0.0%), but semi sterile when F1 males were mated to normal females (per cent egg hatch = 0.03–3.95 %). Low fertility was observed when F1 females were mated with normal males (per cent egg hatch = 130–15.04%). The cytogenetical investigations showed spermatogenesis to proceed normally in the F1 males, whose fathers had been irradiated (0.2 kGy). However, the primary spermatocyte cells carried multiple chromosomal translocations which were the main cause of the sterility in the F1 males.

Key Words

Inherited sterility F1-sterility Ephestia cautelici stored product pest gamma radiation 


La stérilité partielle a été provoquée par l’irradiation de gamma chez les cing mâles adultes de Ephestia Cautella souches de laboratoire (nommées A, B, C, D, et E). Les adults deces souches exposent une visible pigmentation sur l’aile antérieure commeune variation génétique. Les quatre premeiére souches ont été élevées dans le laboratoire plusque 80 génération, tandisque la cinquième jusquâ 10 génération. Léclosion des aeufs ont été diminuées significativement qundune femelle normal croisée avec un mâle irradié de 0.2 kGy de rayons gamma en corn poraison avec celles non-irradiee. On a constaté que l’effet d’irradiation sur léclosion des oeufs de souche C soit plusque lesautres, mais l’effet a diminue progressivement sur les A, E, D, et B, la proportion de sex on té té dénaturée (2 mâle:l femelle) chertoutes les souches après lirradiation, mais la frequence delé-closion et l’union restaient presque normal. D’autre côté la stérilité était total chez les F1 de toutes souches quand on se croisé entre aux, et entre 0.0–3.95% quand une femelle normal croisée avec une F1 maie irradié. On observé ainssi, une faible fertilité quand les deux F1 normal saccoupler (eclosion de 1.3 a 15.04%).

Finalement, notre etudent cytogénétiques ont montrées quele déroulement delà Spermatogenese était normal chez les mâles des F1 provenant de souches irradiées, mais leur cellule spermatocyte apportent une translocation multiple celle aberration chromosomique aète très prolalement la cause principle delà stérilité chez les maies eu F1.


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Copyright information

© ICIPE 1989

Authors and Affiliations

  • Ayad A. Al-Taweel
    • 1
  • Mohammed S. H. Ahmed
    • 1
  • Sarab S. Kadhum
    • 1
  • Asaad A. Hameed
    • 1
  • Majdolin J. Nasser
    • 1
  1. 1.Agriculture and Biology FacultyBaghdadIraq

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