Control of the Coconut Pest Oryctes Rhinoceros L. Using the Oryctes Virus

  • Murali GopalEmail author
  • Alka Gupta
  • B. Sathiamma
  • C. P. Radhakrishnan Nair
Review Article


The coconut palm is an important plantation crop in India, where it is cultivated on 1.796 million hectares. The rhinoceros beetle, Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) is a serious pest of coconut throughout India and southeast Asia, causing an estimated 10% yield loss in the crop. Successful biological control of this pest could be achieved using the non-occluded Oryctes virus (syn. Baculovirus oryctes or Oryctes baculovirus). This review provides an account of this microbial agent, its biology, effects and impact, production and maintenance and alternative hosts, particularly in the context of the Indian situation. It also proposes future areas for investigation on the virus, in order to achieve its commercial viability and more widespread use.

Key words

coconut palm Oryctes rhinoceros Oryctes virus microbial control India 


Le palmier cocotier est une importante culture de plantation en Inde, où il est cultivé sur 1,796 millions d’hectares. Le scarabée rhinocéros, Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) est un sérieux ravageur du cocotier en Inde et en Asie du Sud Est, responsable de pertes de récolte estimées à 10%. Un contrôle biologique efficace de ce ravageur pourrait être obtenu en utilisant le virion non inclus du virus d’Oryctes (syn. Baculovirus oryctes ou baculovirus de l’Oryctes). Cette revue bibliographique fait le point sur cet agent microbien, sa biologie, son efficacité et son impact, sa production et sa maintenance ainsi que sur ses hôtes alternatifs, en particulier dans le contexte de l’Inde. Elle propose également de futurs domaines de recherches sur ce virus, afin d’aboutir à sa viabilité commerciale et à une plus grande utilisation.

Mots Clés

palmier cocotier Oryctes rhinoceros le virus de l’Oryctes Control microbien Inde 


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  1. Abraham C. C. (1994) Pests of coconut and arecanut, pp. 715–716. In Advances in Horticulture Vol. 10 (Edited by K. L. Chadha and P. Rethinam), Malhotra Publishing House, New Delhi, India.Google Scholar
  2. Alfiler A. R. (1990) Virulence of some Philippine isolates of the baculovirus of Oryctes, p. 193. In Proceedings of VI International Colloquim on Invertebrate Pathology and Microbial Control. 20–24 August, 1990, Adelaide, Australia. Glen Osmond, Australia, Dept. of Entomology. University of Adelaide.Google Scholar
  3. Anonymous (1973) The use of virus for the control of insect pests and disease vectors. FAO/WHO Tech. Report Ser. 531, 48. pp.Google Scholar
  4. Bakthavatsalam N. and Sundararaju D. (1990) Pathogenicity of Oryctes baculovirus to cashew stem and root borer, J. Biol. Cont. 4, 127–129.CrossRefGoogle Scholar
  5. Bedford G. O. (1973) Experiments with the virus Rhabdionvirus oryctes against the coconut palm rhinoceros beetle Oryctes rhinoceros and Scapanes australis grossipunctatus in New Guinea, J. Invertebr. Pathol. 22, 70–74.CrossRefGoogle Scholar
  6. Bedford G. O. (1976) Use of virus against the coconut palm rhinoceros beetle in Fiji. PANS 22, 11–25.CrossRefGoogle Scholar
  7. Bedford G. O. (1981) Control of the rhinoceros beetle by baculovirus, pp. 409–426. In Microbial Control of Pests and Plant Diseases, 1970–1980 (Edited by H.D. Burges). Academic Press, London.Google Scholar
  8. Babjan Biju, Sudhadevi K., Dangar T. K., Sathiamma B. and Pillai G. B. (1993) Effect of baculovirus infection on carbohydrates, protein and amino acid levels, and protease activity in haemolymph of Oryctes rhinoceros grubs. J. Plantn. Crops 21, 88–96.Google Scholar
  9. Babjan Biju, Sudhadevi K., Dangar T. K. and Sathiamma B. (1995) Biological suppression of Oryctes rhinoceros by re-release of Baculovirus oryctes in an infected contiguous area. J. Plantn. Crops 23, 62–63.Google Scholar
  10. Bonning B. C. and Hammock B. D. (1996) Development of recombinant baculoviruses for insect control. Annu. Rev. Entomol. 41, 191–210.PubMedCrossRefGoogle Scholar
  11. Caltagirone L.S. (1981) Landmark examples in classical biological control. Annu. Rev. Entomol. 26, 213–232.CrossRefGoogle Scholar
  12. Crawford A. M. (1981) Attempts to obtain Oryctes baculovirus replication in three insect cell cultures. Virol. 112, 625–633.CrossRefGoogle Scholar
  13. Crawford A. M. (1982) A coleopteran cell line derived from Heteronychus arator (Coleoptera: Scarabaeidae). In Vitro 18, 813–816.CrossRefGoogle Scholar
  14. Crawford A. M. and Sheehan C. (1985) Replication of Oryctes baculovirus in cell culture: viral morphogenesis, infectivity and protein synthesis, J. Gen. Virol. 66, 529–539.CrossRefGoogle Scholar
  15. Crawford A. M. and Zelazny B. (1990) Evolution of Oryctes baculovirus: rate and types of genomic change. Virol. 174 (1), 294–298.CrossRefGoogle Scholar
  16. Crawford A. M., Ashbridge K., Sheehan C. and Faulkner P. (1985) A physical map of the Oryctes baculovirus genome, J. Gen. Virol. 66, 2649–2658.CrossRefGoogle Scholar
  17. Dangar T. K., Solomon J. J. and Pillai G. B. (1994) Infection of the coconut palm beetle, Xylotrupes gideon (Coleoptera: Scarabaeidae) by a nonoccluded baculovirus. Z. Pflankranh. Pflschutz. 101(6), 561–566.Google Scholar
  18. David W. A. L. (1975) The status of viruses pathogenic for insects and mites. Annu. Rev. Entomol. 20, 97–117.PubMedCrossRefGoogle Scholar
  19. Dhileepan K. (1994) Impact of release of Baculovirus oryctes into the population of Oryctes rhinoceros in an oil-palm plantation in India. Planter 70(819), 255–266.Google Scholar
  20. Evans H. and Shapiro M. (1997) Viruses, pp. 17–52. In Manual of Techniques in Insect Pathology (Edited by L. A. Lacey ). Academic Press, San Diego, California.CrossRefGoogle Scholar
  21. Francku R. I. B., Fauquet C. M., Knudson D. L. and Brown F. (1991) Classification and nomenclature of viruses. Fifth report of the International committee on Taxonomy of Viruses. Arch. Virol. (Suppl.2)., Springer Verlag, Vienna.Google Scholar
  22. Gopal M. and Gupta A. (2000) Pseudomonas alcaligenes infection in grubs dampers natural transmission of Baculovirus in Oryctes rhinoceros L. In Natural Resources Vol. 2. Proceedings of International Conference on Managing Natural Resources for Sustainable Agricultural Production in the 21st Century. Feb. 14–18, 2000, New Delhi, India (Edited by J.S.P. Yadav and G. Narayanasamy). Cambridge Printing Works, New Delhi, India. 722 pp.Google Scholar
  23. Gopal M. and Sathiamma B. (2000) Coir waste: an alternative feed material for maintenance of Baculovirus inoculated rhinoceros beetle, Oryctes rhinoceros (L), pp. 339–341. In Recent Advances in Plantation Crops Research. Proceedings of the 13th Plantation Crops Symposium (PLACROSYM XIII). Dec 16–18, 1998, Coimbatore, India (Edited by N. Muraleedharan and R. Rajkumar). Allied Publishers Ltd., Chennai, India.Google Scholar
  24. Gorick B. D. (1980) Release and establishment of the baculovirus disease of Oryctes rhinoceros (L.) (Coleoptera Scarabaeidae) in Papua New Guinea. Bull. Ent. Res. 70, 445–453.CrossRefGoogle Scholar
  25. Gourreau J. M., Kaiser C., Lahellec Mv, Chevrier L. and Monsarrat P. (1979) Study of the possible pathogenic action of the Baculovirus affecting Oryctes on pigs. Entomophaga 24, 213–219.CrossRefGoogle Scholar
  26. Gourreau J. M., Kaiser C. and Monsarrat P. (1982) Study of the possible pathogenic action of the Oryctes baculovirus in the white mouse. Annales de Virologie, E. 133, 423–428.Google Scholar
  27. Hammes C. (1971) Multiplication et introduction d’un virus d’Oryctes rhinoceros a l’Ile Wallis. Cr Hebd. Seanc. Acad. Sci., Paris (D) 273, 1048–1050.Google Scholar
  28. Hochberg M. E. and Waage J. K. (1991) A model of the biological control of Oryctes rhinoceros (Coleoptera: Scarabaeidae) by means of pathogens, J. Appl. Ecol. 28, 514–531.CrossRefGoogle Scholar
  29. Huger A. M. (1966) A virus disease of the Indian rhinoceros beetle, Oryctes rhinoceros (Linnaeus), caused by a new type of insect virus, Rhabdionvirus oryctes, gen. n., sp.n. J. Invertebr. Pathol. 8, 38–51.PubMedCrossRefGoogle Scholar
  30. Jacob T. K. (1996) Introduction and establishment of baculovirus for the control of rhinoceros beetle Oryctes rhinoceros (Coleoptera: Scarabaeidae) in the Andaman Islands (India). Bull. Ent. Res. 86, 257–262.CrossRefGoogle Scholar
  31. John V. T. (1965) A micro immuno-osmophoresis technique for assay of tobacco mosaic virus. Virology 27, 121–123.PubMedCrossRefPubMedCentralGoogle Scholar
  32. Julia J. F. and Mariau D. (1976) Research on Oryctes monoceros 01. in Ivory Costa II. Trial of biological control by virus Rhabdionvirus oryctes. Oleagineux 31, 113–117.Google Scholar
  33. Kelly D. C. (1976) Oryctes virus replication: electron microscopic observations on infected moth and mosquito cells. Virology 69, 596–606.PubMedCrossRefPubMedCentralGoogle Scholar
  34. Klein M. G. and Lacey L. A. (1999) An attractant trap for autodissemination of entomopathogenic fungi into populations of the Japanese beetle Popillia japonica (Coleoptera: Scarabaeidae). Biocontrol Sci. Technol. 9, 151–158.CrossRefGoogle Scholar
  35. Kurian C., Sathiamma B. and Pillai G. B. (1979) World distribution of pests of coconut. Technical Document No: 119. FAO Plant Protection Committee for the South East Asia and Pacific Region, 53 pp.Google Scholar
  36. Lomer C. J. (1986) Release of Baculovirus oryctes into Oryctes monoceros population in the Seychelles, J. Invertebr. Pathol. 47, 237–246.CrossRefGoogle Scholar
  37. Longworth J. F. and Carey G. P. (1980) The use of an indirect enzyme-linked immunosorbent assay to detect baculovirus in larvae and adults of Oryctes rhinoceros from Tonga, J. Gen. Virol. 47, 431–438.CrossRefGoogle Scholar
  38. Majumder N. D. and Jacob T. K. (1993) Virus sensitive sites in the chromosomes of rhinoceros beetle (Oryctes rhinoceros L.). Nucleus (Calcutta) 36, 66–68.Google Scholar
  39. Marschall K. J. (1970) Introduction of new virus disease of the coconut rhinoceros beetle in Western Samoa. Nature 225, 288–289.CrossRefGoogle Scholar
  40. Mohan K. S. and Gopinathan K. P. (1989a) Quantitation of serological cross-reactivity between two geographical isolates of Oryctes baculovirus by a modified ELISA, J. Virol. Meth. 24, 203–214.CrossRefGoogle Scholar
  41. Mohan K. S. and Gopinathan K. P. (1989b) Characterization of viral proteins of Oryctes baculovirus and comparison between two geographical isolates. Arch. Virol. 109, 207–222.PubMedCrossRefGoogle Scholar
  42. Mohan K. S. and Pillai G. B. (1983) Immunoosmophoresis technique for quick diagnosis of Oryctes virus (Baculoviridae) of rhinoceros beetle Oryctes rhinoceros L. Indian J. Exp. Biol. 21, 470–471.Google Scholar
  43. Mohan K. S., Jayapal S. P. and Pillai G. B. (1983) Baculovirus disease in Oryctes rhinoceros population in Kerala, J. Plantn. Crops 11, 154–161.Google Scholar
  44. Mohan K. S., Jayapal S. P. and Pillai G. B. (1985a) Response of Oryctes rhinoceros larvae to infection by Oryctes baculovirus. J. Plantn. Crops 13, 116–124.Google Scholar
  45. Mohan K. S., Jayapal S. P. and Pillai G. B. (1985b) Diagnosis of baculovirus infection in coconut rhinoceros beetles by examination of excreta. Z. Pflkranh. Pflschutz. 93, 379–383.Google Scholar
  46. Mohan K. S., Jayapal S. P. and Pillai G. B. (1989) Biological suppression of coconut rhinoceros beetle Oryctes rhinoceros (L) in Minicoy, Lakshadweep by Oryctes baculovirus: Impact on pest population and damage, J. Plantn. Crops 16 (Suppl.), 163–170.Google Scholar
  47. Mohan K. S. and Gopinathan K. P. (1991) Physical mapping of the genomie DNA of the Oryctes rhinoceros baculovirus, KI. Gene 107, 343–344.PubMedCrossRefGoogle Scholar
  48. Mohan K. S. and Gopinathan K. P. (1992) Characterization of the genome of Oryctes Baculovirus, a viral biocide of the pest Oryctes rhinoceros. J. Biosci. 17, 421–430.CrossRefGoogle Scholar
  49. Mohan K. S. and Pillai G. B. (1993) Biological control of Oryctes rhinoceros (L.) using an Indian isolate of Oryctes baculovirus. Insect Sci. Applic. 14, 551–558.Google Scholar
  50. Monsarrat P., Meynadier G., Croizier G. and Vago C. (1973a) Recherches cytopathologiques sur une maladie virale du Coléoptère Oryctes rhinoceros L. C.r. hebd. Seanc. Acad. Sci., Paris D 276, 2077–2080.Google Scholar
  51. Monsarrat P., Veyrunes T., Meynadier G., Croizier G. and Vago C. (1973b) Purification et étude structurale du virus du Coléoptère Oryctes rhinoceros L. Cr. Hebd. Seanc. Acad. Sci., Paris D 277, 1413–1415.Google Scholar
  52. Monsarrat P., Duthoit J. L. and Vago C. (1974) Mise en évidencede virions de type Baculovirus dans l’appareil génital du Coléoptère Oryctes rhinoceros L. Cr. Hebd. Seanc. Acad. Sci. Paris D 278, 3259–3261.Google Scholar
  53. Monsarrat P. and Veyrunes J. C. (1976) Evidence of Oryctes virus in adult feces and new data of virus characterizations. J. Invertebr. Pathol. 27, 387–389.CrossRefGoogle Scholar
  54. Monty J. (1978) The coconut palm rhinoceros beetle Oryctes rhinoceros (L.) in Mauritius, and its control. Rev. Agric. Entol. Ile Maurice 57, 60–76.Google Scholar
  55. Nair M. R. G. K. (1986) Insects and Mites of Crops of India. Indian Council of Agricultural Research (ICAR). New Delhi, India, p. 83.Google Scholar
  56. Nirula K. K. (1955) Investigations on the pests of coconut palm. Part II. The Oryctes rhinoceros. L. Indian Cocon. J. 8, 161–180.Google Scholar
  57. Paulose S., Abraham C. C. and Paulose S. (1997) Effect of Baculovirus oryctes (Kerala isolate) infection on consumption and its utilization of food by Oryctes rhinoceros grubs. Indian J. Entomol. 59, 311–315.Google Scholar
  58. Payne C. C. (1974) The isolation and characterization of a virus from Oryctes rhinoceros. J. Gen. Virol. 25, 105–116.PubMedCrossRefGoogle Scholar
  59. Payne C. C., Compson D. and De Looze S. M. (1977) Properties of the nucleocapsids of a virus isolated from Oryctes rhinoceros. Virol. 77, 269–280.CrossRefGoogle Scholar
  60. Purrini K. (1989) Baculovirus oryctes release into Oryctes monoceros population in Tanzania with special reference to interaction of virus isolates used in our laboratory infection experiments, J. Invertebr. Pathol. 53, 285–300.CrossRefGoogle Scholar
  61. Quiot J., Monsarrat P., Meynadier G., Croizier G. and Vago C. (1973) Infection des cultures cellulaires des Coléoptères par le virus Oryctes. Comptes rendus hebdomadaires des séancea de l’Académie des Sciences, Serie D. 276, 3229–3231.Google Scholar
  62. Rajamannar M. and Indiravathi G. (2000) Detection of baculovirus infection in rhinoceros beetle (Oryctes rhinoceros L.) through DAC-indirect ELISA and DIBA. J. Plantn. Crops 28, 89–93.Google Scholar
  63. Ramachandran C. P., Kurian C. and Mathew J. (1963) Assessment of damage to coconuts due to Oryctes rhinoceros. L. Nature of damage caused by the beetle and factors involved in the estimation of loss. Indian Cocon. J. 17, 3–12.Google Scholar
  64. Revet B. and Monsarrat P. (1974) L’acide nucléique du virus du Coléoptère Oryctes rhinoceros L.: un ADN superhelicoidal de haut poids moleculaire. Cr. Hebd. Seanc. Acad. Sci. Paris D 278, 331–334.Google Scholar
  65. Richards N. K., Glare T.R., Aloali’i I. and Jackson T. A. (1999) Primers for the detection of Oryctes virus from Scarabaeidae (Coleoptera). Molec. Ecol. 8, 1552.CrossRefGoogle Scholar
  66. Shukla R. P., Tandon P. L. and Singh S. J. (1984) Baculovirus a new pathogen of mango nut weevil Sternochetus mangiferae (F.) (Coleoptera: Curculionidae). Curr. Sci. 53, 593–594.Google Scholar
  67. Villacarlos L. T. and Betonio P. A. (1990) Incidence of baculovirus and green muscardine fungus in the larval population of rhinoceros beetle in Baybay, Leyte, Philippines. Philippine J. Cocon. Studies XV (1), 21–23.Google Scholar
  68. Vincent V. M. J., Ali S. H. and Lakshmanan M. (1988) Baculovirus infection of Oryctes rhinoceros. Effect of virus on the total and differential haemocyte counts. J. Biol. Cont. 2, 29–32.Google Scholar
  69. Young E.C. (1974) The epizootology of two pathogens of the coconut palm rhinoceros beetle, J. Invertebr. Pathol. 24, 82–92.PubMedCrossRefGoogle Scholar
  70. Zelazny B. (1972) Studies on Rhabdionvirus oryctes I. Effect on larvae of Oryctes rhinoceros and inactivation of the virus. J. Invertebr. Pathol. 20, 235–241.CrossRefGoogle Scholar
  71. Zelazny B. (1973a) Studies on Rhabdionvirus oryctes. II. Effect on adults of Oryctes rhinoceros, J. Invertebr. Pathol. 22, 122–126.CrossRefGoogle Scholar
  72. Zelazny B. (1973b) Rhinoceros Beetle Project. Annu. Report, p, 62–63 UNDP/FAO. Apia, Western Samoa.Google Scholar
  73. Zelazny B. (1976) Transmission of a baculovirus in populations of Oryctes rhinoceros. J. Invertebr. Pathol. 27, 221–227.CrossRefGoogle Scholar
  74. Zelazny B. (1978) Methods of inoculating and diagnosing the baculovirus disease of O. rhinoceros. FAO Plant Prot. Bull. 26, 163–168.Google Scholar
  75. Zelazny B. (1979) Loss in coconut yield due to Oryctes rhinoceros damage. FAO Plant Prot. Bull. 27, 65–70.Google Scholar
  76. Zelazny B. (1981) India—presence of the baculovirus of Oryctes rhinoceros. FAO Plant Prot. Bull. 29, 77–78.Google Scholar
  77. Zelazny B., Alfiler A. R. and Mohamed N. A. (1985) Glass permeation chromatography for purification of the baculovirus of Oryctes rhinoceros (Coleoptera: Scarabaeidae). J. Econ. Entomol. 78, 992–994.CrossRefGoogle Scholar
  78. Zelazny B. and Alfiler A. R. (1986) Oryctes rhinoceros (Coleoptera: Scarabaediae) larva abundance and mortality factors in the Philippines. Environ. Entomol. 15, 84–87.CrossRefGoogle Scholar
  79. Zelazny B., Alfiler A. R. and Crawford A. M. (1987) Preparation of baculovirus inoculum for use by coconut farmers to control rhinoceros beetle (Oryctes rhinoceros). FAO Plant Prot. Bull. 35, 36–42.Google Scholar
  80. Zelazny B., Avtar M. L., Singh Rajendra and Malone L. A. (1988) Papuana uninodis, a new host for the baculovirus of Oryctes. J. Invertebr. Pathol. 51, 157–160.CrossRefGoogle Scholar
  81. Zelazny B., Alfiler A. R. and Lolong A. (1989) Possibility of resistance to a baculovirus in populations of the coconut rhinoceros beetle (Oryctes rhinoceros). FAO Plant Prot. Bull. 37, 77–82.Google Scholar
  82. Zelazny B., Lolong A. and Crawford A. M. (1990) Introduction and field comparison of baculovirus strains against Oryctes rhinoceros (Coleoptera: Scarabaeideae) in the Maldives. Environ. Entomol. 19, 1115–1121.CrossRefGoogle Scholar
  83. Zelazny B., Lolong A. and Pattang B. (1992) Oryctes rhinoceros (Coleoptera: Scarabaeidae) populations suppressed by baculovirus. J. Invertebr. Patliol. 59, 61–68.CrossRefGoogle Scholar

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© ICIPE 2001

Authors and Affiliations

  • Murali Gopal
    • 1
    Email author
  • Alka Gupta
    • 1
  • B. Sathiamma
    • 1
  • C. P. Radhakrishnan Nair
    • 1
  1. 1.Central Plantation Crops Research InstituteKayangulam, KrishnapuramIndia

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