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Integrity of the 130 Kda Polypeptide of Bacillus Thuringiensis Ssp. Israelensis δ-Endotoxin in K-S Sporulation Medium

  • Z. Ngalo Otieno-Ayayo
  • David M. Chipman
  • Arieh Zaritsky
  • Kamal Khawaled
Research Article

Abstract

The 130 kDa polypeptide appears to be the most susceptible of the polypeptides constituting the Bacillus thuringiensis ssp. israelensis δ-endotoxin to the proteases associated with sporulation. Stabilization of this polypeptide in the recently described K-S sporulation medium has been achieved through limiting its cleavage by removing contaminating proteases from the culture by washing. In this way, the complete δ-endotoxin profile is retained for studies on all of the constituent polypeptide fractions.

Key Words

Bacillus thuringiensis israelensis 130 kDa polypeptide disappearance and stabilization K-S medium 

Résumé

Parmi les polypeptides constituant δ-endotoxine de Bacillus thuringiensis israelensis, le polypeptide de poids moléculaire 130 kDa semble être le plus vulnérable aux proteases associées á la sporulation. La stabilisation de ce polypeptide par le procéde récemment décrit, “milieu de sporulation K-S”, a été obtenue en limitant sa dégradation par lavage des protéase ayant contaminées de milieu de culture. Ainsi, le profile complet de δ-endotoxine a été retenu par études sur toutes les composantes des polypeptides.

Mots Clés

Bacillus ssp. thuringiensis israelensis polypeptide deolypeptide de poids moléculaire 130 kDa disparition et stabilisation milieu K-S 

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Copyright information

© ICIPE 1993

Authors and Affiliations

  • Z. Ngalo Otieno-Ayayo
    • 1
  • David M. Chipman
    • 1
  • Arieh Zaritsky
    • 1
  • Kamal Khawaled
    • 1
  1. 1.Department of Life SciencesBen Gurion University of the NegevBeer ShevaIsrael

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