Advertisement

Differential Susceptibility of Parasitized and Unparasitized Larvae of Spodoptera Littoralis (Boisd.) (Lepidoptera: Noctuidae) to Its Nuclear Polyhedrosis Virus

  • Ahlam A. M. Al-Fazairy
  • E. M. Hegazi
  • Hedaya H. Karam
  • Lamya M. Abo-Abd-Allah
Research Article

Abstract

Rates of virus-induced mortality and values of the LC50 and LT50 showed that, in general, parasitism by Microptitis rufiventris Kok. increased the susceptibility of the Egyptian cotton leafworm, Spodoptera littoralis second-instar larvae to its nuclear polyhedrosis virus (SLNPV). By comparing the LC50values, data revealed that virus-infected parasitized larvae were ca. 3.5 and 4.8 times more susceptible to SLNPV than virus-infected unparasitized ones at 8 and 12 days post-infection, respectively. Also, in virus-infected unparasitized larvae, the time required to attain a 50% mortality was significantly longer than that in virus-infected parasitized ones.

Key Words

Spodoptera littoralis Microplitis rufiventris nuclear polyhedrosis virus susceptibility 

Résumé

Virus-induirés mort (%) et les valeurs de LC50 et LT50 révélé que parasitism par Microplitis rufiventris Kok. augmenta le sensible d’une feuille de coton égyptien, Spodoptera littoralis II - stade larvés au le noyau polyhedrosé virus (NPV). Les valeurs de LC50 ont révélé que virus - infectés parasitiés larvés était plus sensible 3.5 et 4.8 fois que les virus - infectés non-parasitiés larvés après 8 et 12 jours d’infection respectivement. Aussi, pour les virus - infectés non-parasitiés larvés, la valeur de LT50 était significantly longuer que les virus-infectes parasitiés larvés.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Beegle C. C. and Oatman E. R. (1974) Differential susceptibility of parasitized and non-parasitized larvae of Trichoplusia ni to a nuclear polyhedrosis virus. J. lnvertebr. Pathol. 24, 188–195.CrossRefGoogle Scholar
  2. Eller F. J., Boucias D. G. and Tumlinson J. H. (1988) Interactions between Microplitis croceipes (Hymenoptera: Braconidae) and a nuclear polyhedrosis virus of Heliothis zea (Lepidoptera: Noctuidae). Environ. Entomol. 17, 977–982.CrossRefGoogle Scholar
  3. Hafez M, Kamel A. A. M, Mostafa T. H. and Omar E. E. (1970) Field test of combination of polyhedrosis virus suspensions and certain chemical insecticides for control of the cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). Bull. Soc. Entomol. Egypt 4, 65–69.Google Scholar
  4. Harpaz I. and Raccah B. (1978) Nucleopolyhedrosis virus (NPV) of the Egyptian cottonworm, Spodoptera littoralis (Lepidoptera: Noctuidae): Temperature and pH relations, host range and synergism. J. lnvertebr. Pathol. 32, 368–372.CrossRefGoogle Scholar
  5. Hassan S. M. and Moawed S. M (1974) Toxicological studies on the effect of nuclear polyhedrosis for control of the cotton leafworm, Spodoptera littoralis (Boisd.). Symposium on the Use of Isotopes in Pesticides and Pest Control. Beirut, Lebanon, pp. 175–180.Google Scholar
  6. Hedlund R. C. and Yendol W. G. (1974) Gypsy moth nuclear polyhedrosis virus production as related to inoculating time, dosage, and larval weight. J. Econ. Entomol. 67, 61–63.CrossRefGoogle Scholar
  7. Hegazi E. M. and El-Minshawy A. M. (1979) Laboratory technique for mass rearing Microplitis rufiventris Kok. (Braconidae: Hymenoptera) an internal parasite of the cotton leafworm Spodoptera littoralis (Boisd.) (Noctuidae: Lepidoptera). Boll. Lab. Entomol. Agr. “F. Silvestri”, 36, 205–210.Google Scholar
  8. Hegazi E. M., Hammad S. M. and El-Minshawy A. M. (1977) Field and laboratory observations on the parasitoids of Spodoptera littoralis (Boisd.) (Lep.: Noctuidae) in Alexandria. Z. Angew. Entomol. 84, 316–327.CrossRefGoogle Scholar
  9. Hegazi E. M., Hammad S. M., Altahtawy M. M. and El-Sawaf S. K. (1973) Parasites of the larval stage of the cotton leafworm Spodoptera littoralis (Boisd.) (Noctuidae: Lepidoptera) in Alexandria region. Z. Angew. Entomol. 74, 332–336.CrossRefGoogle Scholar
  10. Klein M. and Podoler H. (1978) Studies on the application of a nuclear polyhedrosis virus to control population of the Egyptian cottonworm Spodoptera littoralis. J. Invertebr. Pathol. 32, 244–248.CrossRefGoogle Scholar
  11. Litchfield J. T. and Wilcoxon F. (1949) A simplified method of evaluating dose-effect experiments. J. Pharmacol. Exp. Therap. 96, 99–133.Google Scholar
  12. Salama H. S., Moawed S. M. and Zaki F. N. (1987) Effects of nuclear polyhedrosis virus-Bacillus thuringiensis combinations on Spodoptera littoralis (Boisd.). J. Appl. Entomol. 104, 23–27.CrossRefGoogle Scholar
  13. Tawfik M. F. S.; Hafez M. and Ibrahim A. A. (1977) The bionomics of Microplitis rufiventris. Bull. Soc. Entomol. Egypt 61, 123–136.Google Scholar
  14. Teakle R. E. Jensen J. M. and Mulder J. C. (1985) Susceptibility of Heliothis armigera (Lepidoptera: Noctuidae) on sorghum to nuclear polyhedrosis virus. J. Econ. Entomol. 78, 1373–1378.CrossRefGoogle Scholar
  15. Vail P. V., Soo Hoo C. F., Seay R. S., Killinen R. G. and Wolf W. W. (1972) Microbial control of Lepidopterous pests of fall lettuce in Arizona and effects of chemical and microbial pesticides on parasitoids. Environ. Entomol. 1, 780–785.CrossRefGoogle Scholar
  16. Vail P.V., Jay D.L., Stewart F.D., Martinez A.J. and Dulmage H. (1978) Comparative susceptibility of Heliothis virescens and H. zea to the nuclear polyhedrosis virus isolated from Autogrpha californica. J. Econ. Entomol. 71, 293–296.CrossRefGoogle Scholar
  17. Verenini M. (1983) Development of parasitiods in hosts affected by infectious diseases. Bollettino dell’Instituto di Entomologia della Universita degli Studi di Bologna, 37, 225–265 (cf. Rev. Appl. Entomol. 1984, 72, 1735.Google Scholar

Copyright information

© ICIPE 1993

Authors and Affiliations

  • Ahlam A. M. Al-Fazairy
    • 1
  • E. M. Hegazi
    • 1
  • Hedaya H. Karam
    • 1
  • Lamya M. Abo-Abd-Allah
    • 1
  1. 1.Faculty of Agriculture, Plant Protection Department, Entomology DivisionUniversity of AlexandriaAlexandriaEgypt

Personalised recommendations