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Experimental Transmission of Ife Virus by Aedes Aegypti Mosquitoes

  • G. O. Ezeifeka
  • J. U. Umoh
  • C. D. Ezeokoli
  • N. E. Gomwalk
Research Article

Abstract

Transmission of Ife virus by Aedes aegypti mosquitoes was investigated. Mosquitoes were experimentally infected by feeding on viraemic mice or on infected mouse brain suspended in defibrinated chick blood. Normal infant mice were exposed to infected mosquitoes after predetermined extrinsic incubation period. Ife virus persisted for only 1 day in mosquitoes that fed on viraemic mice and for 5 days in those that fed on infected brain/blood suspension. Virus was not recovered in infant mice exposed to infected mosquitoes. However, there was serological evidence of virus transmission manifested as the presence of complement fixing antibody against Ife virus in mice exposed after 2 days and 2–5 days extrinsic incubation periods to mosquitoes infected by feeding on viraemic mice and by feeding on infected brain/blood suspension respectively. This transmission was considered mechanical in view of the short virus persistence and extrinsic incubation periods.

Key Words

Aedes aegypti Ife virus transmission 

Résumé

La transmission du virus d’Ife par les moustiques de l’espèce Aedes aegypti a été étudiée. Ces moustiques ont été expérimentalement infectés en les nourrisant de souris viraemiques, ou de matière cérébrale (cervelle) de souris infectées dans une suspension de sang defibriné de poulet. Des jeunes souris ont été exposées aux moustiques infectés après la période d’incubation extrinsique prédéterminée.

Dans les moustiques nourris de souris viraemiques, le virus d’Ife n’a persisté qu’un jour, contre cinq jours pour ceux nourris de la suspension infectée cervelle/sang. Aucun virus n’a été retrouvé dans les jeunes souris exposées aux moustiques infectés. Cependant, on a pu trouver une evidence de transmission du virus, ceci se manifestant par la présence d’anticorps complémentaires contre le virus d’Ife dans les souris exposées durant des périodes d’incubation extrinsèques de 2 jours et de 2 à 5 jours aux moustiques infectés en les nourrisant de souris viraemlques et de la suspension infectée cervelle/sang respectivement. Cette transmission est considérée comme étant mécanique à cause de les courte durée de la persistence et des périodes d’incubation extrinsèques.

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Copyright information

© ICIPE 1993

Authors and Affiliations

  • G. O. Ezeifeka
    • 1
  • J. U. Umoh
    • 1
  • C. D. Ezeokoli
    • 2
  • N. E. Gomwalk
    • 3
  1. 1.Department of Veterinary Public Health and Preventive MedicineNigeria
  2. 2.Department of Veterinary Surgery and MedicineNigeria
  3. 3.Department of MicrobiologyAhmadu Bello UniversityZariaNigeria

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