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Immunochemical Ablation of Accessory Reproductive Glands of the Male Desert Locust

  • T. S. Dhadialla
  • Thomas R. Odhiambo
  • G. G. Wagner
Research Article

Abstract

Injection of antiserum prepared against accessory reproductive gland (ARG) secretions of the male desert locust, Schistocerca gregaria, disrupted the normal growth and function of the ARG complex in sexually maturing adults. Male locusts were injected with either antiserum or normal guinea-pig serum on days 3, 7 and 10 after adult ecdysis. When the injection of ARG antiserum began as early as day 3 of adult development a marked inhibition of ARG growth was accompanied by the suppression of other characteristics of sexual maturation (yellow body coloration and mating behaviour). Inhibition of ARG growth, while significant, was less pronounced when antiserum injections began on day 7 of adult development, and the injected males did not exhibit normal mating behaviour when placed with virgin females. When single antiserum injections were administered on day 10 of adult development, the males still attained their yellow body coloration by day 19 and copulated normally with virgin females, although their ARGs were only equivalent in size to those 13-day-old males.

Key Words

Immunochemical ablation accessory reproductive glands desert locust 

Résumé

L’injection de l’antiserum préparé contre les sécretions de la glande reproductrice auxilliair (ARG) des locustes males du desert, Schistocerca gregaria, interompt la eroissance normale et le fouctionnement du complexe ARG chezles adultes sexuellement matures. Les locustes mâles sont injectes auee aussi bien l’antiserum qu’avec le serum normal de cobaye aux jours 3, 7 et 10 aprés la transformation adulte. Quand l’injection de l’antiserum ARG commence tres tôt au jour 3 du developpment adulte, une inhibition, marquée de la croissance de l’ARG est accompagnée par la suppression des autres caracteristiques de la maturation sexuelle (une coloration jamme du corps, et un comportement sexuel). L’inhibition de la croissance de l’ARG quoique significative, est moius pronnoucée quand l’injection de l’antiserum de’bute au jour 7 du du developpment adulte et les maies injecte’s néxhibeut pas de compartement sexuel normal quand ils sout placés en presence de females. Quand des injections uniques de l’antiserum sout adminstrées au jour 10 du developpment adulte, les maies gardent eucore leur coloration corporelle jaune jusqu’au jour 19. Et s’accouplent normalement avec les females vierges bien que leurs ARGs sont seulement equivalents en grandeur a’ ceux des males ages de 13 jours.

Mots Cléf

Ablation immunochimique glandes reproductriees auxilliaries des locustes du desert 

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References

  1. Alger E. N. and Cabrera E. J. (1972) An increase in death rate of Anopheles stephensi fed on rabbits immunized with mosquito antigen. J. econ. Ent. 65, 165–168.CrossRefGoogle Scholar
  2. Allen J. R. and Humphreys S. J. (1979) (Immunization of guinea pigs, and cattle against ticks. Nature 280, 491–493.CrossRefGoogle Scholar
  3. Bun Ng T., Campbell C. M. and Idler D. R. (1980) Antibody inhibition of vittellogenesis and oocyte maturation in salmon and flounder. Gen. comp. Endocr. 41, 233–239.CrossRefGoogle Scholar
  4. Gregory G. E. (1965) The formation and fate of the spermatophore in the African migratory locust, Locusta migratoria migratorioides Reiche and Fairmaire. Trans. R. ent. Soc. Lond. 117, 33–66.CrossRefGoogle Scholar
  5. Hagedorn H. H. and Kunkel J. G. (1979) Vitellogenin and vitellin in insects. A. Rev. Ent. 24, 475–505.CrossRefGoogle Scholar
  6. Hartmann R. (1970) Experimentelle und histologische Untersuchungen der spermatophorenbildung beki der Feldheuschrecke Gomphocerus rufus L. (Ortheptera;Acrididae). Z. Morph. Tiere. 68, 140–176.CrossRefGoogle Scholar
  7. Hunter-Jones P. (1966) Rearing and Breeding Locusts in the Laboratory. Anti-Locust Research Centre, London.Google Scholar
  8. Kaaya G. P. and Alemu P. (1982) Fecundity and survival of tsetse maintained on immunized rabbits. Insect Sci. Applic. 3, 237–241.Google Scholar
  9. Loher W. (1961) The chemical acceleration of the maturation process and its hormonal control in the male of the desert locust. Proc. R. Soc. B 153, 380–397.Google Scholar
  10. Lowry O. H., Rosebrough N. J., Farr A. L. and Randall R. J. (1951) Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275.PubMedGoogle Scholar
  11. Norris M. J. (1954) Sexual maturation in the desert locust (Schistocerca gregaria Forskal) with special reference to the effects of grouping. Anti-Locusts Bull. 18, 1–44.Google Scholar
  12. Odhiambo T. R. (1966) Growth and hormonal control of sexual maturation in the male desert locust, Schistocerca gregaria Forskal. Trans. R. ent. Soc. Lond. 118, 393–412.CrossRefGoogle Scholar
  13. Odhiambo T. R. (1969) The architecture of the accessory reproductive glands of the male desert locust. I. Types of glands and their secretions. Tissue Cell 1, 155–182.CrossRefGoogle Scholar
  14. Pener M. P. (1965) On the influence of corpora allata on maturation and sexual behaviour of Schistocerca gragaria. J. Zool. 147, 119–136.Google Scholar
  15. Pener M. P. (1967) Effects of allatectomy and sectioning of the nerves of the corpora allata on oocyte growth, male sexual behaviour and colour change in adults of Schistocerca gregaria. J. Insect Physiol. 13, 665–684.CrossRefGoogle Scholar
  16. Pickford R. and Padgham D. (1973) Spermatophore formation and spermtransfer in the desert locust, Schistocerca gregaria (Orthoptera; Acrididae). Can. Ent. 105, 613–719.CrossRefGoogle Scholar
  17. Rembold H., Eder J. and Ulrich G. M. (1980) Inhibition of allatotropic activity and ovary development in Locusta migratoria by anti-brain-antibodies. Z. Naturforsch. 35c, 1117–1119.CrossRefGoogle Scholar
  18. Scheidegger J. J. (1955) Une micro-methode de l’immuno-electrophrese. Inter. Arch. Allergy Appl. Immun. 7, 103–110.CrossRefGoogle Scholar
  19. Schiein Y. and Lewis C. T. (1976) Lesions in hematophagus flies after feeding on rabbits immunized with fly tissues. Physiol. Ent. 1, 55–59.CrossRefGoogle Scholar
  20. Shirk P. D., Dahm K. H. and Roller H. (1976) The accessory sex glands as repository for juvenile hormone in male cecropia moths. Z. Naturforsch. 31c, 199–200.CrossRefGoogle Scholar
  21. Shivers C. A., Dudkiewicz A. B., Franklin L. E. and Fussell E. N. (1972) Inhibition of sperm-egg interaction by specific antibody. Science 178, 1211–1213.CrossRefGoogle Scholar
  22. Sutherland G. B. and Ewen A. B. (1973) Fecundity decrease in mosquitoes ingesting blood from specifically sensitized mammals. J. Insect Physiol. 20, 655–660.CrossRefGoogle Scholar
  23. Wikel S. K. (1982) Immune responses to arthropods and their products. A. Rev. Ent. 27, 21–48.CrossRefGoogle Scholar
  24. Wikel S. K. (1984) Immunomodulation of host responses to ectoparasite infestation—an overview. Vet. Parasit. 14, 321–339.CrossRefGoogle Scholar
  25. Willadsen P. (1980) Immunity to ticks. Adv. Parasit. 18, 293–313.CrossRefGoogle Scholar
  26. Wyatt G. R. and Pan M.-L. (1978) Insect plasma proteins. A. Rev. Biochem. 47, 779–817.CrossRefGoogle Scholar

Copyright information

© ICIPE 1986

Authors and Affiliations

  • T. S. Dhadialla
    • 1
  • Thomas R. Odhiambo
    • 1
  • G. G. Wagner
    • 1
  1. 1.The International Centre of Insect Physiology and Ecology (ICIPE)NairobiKenya

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