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Thyroxine-Induced Alterations in Protein and Nucleic Acid Contents of Fat Body of Female Silkworms During Different Developmental Stages

  • Anathbandhu Chaudhuri
  • A. K. Medda
Research Article

Abstract

The fat body protein content of silkworms increased with 0.5 μg of thyroxine (T4) per gram of body weight and more so with 1 μg/g on days 4 and 8 after injection. Thyroxine with 1 μg/g dose, however, caused an earlier increase in protein content on day 2. The higher doses of T4 (2 and 5 μg/g) decreased the protein content. All T4 treated groups (except with an ineffective dose of 0.25 μg/g) showed reduced protein level in the adult stage in comparison to the control. Thyroxine with a dose of 0.25, 0.5 and 1 μg/g increased the fat body RNA content over the control level which was observed on days 2, 4 and 8. But on day 13, the RNA content in these groups returned to the control level. Higher doses of T4 (2 and 5 μg/g) decreased the RNA content during the developmental period. DNA content was not altered by lower doses of T4 (0.25,0.5 and 1 μg/g), while the higher doses (2 and 5 μg/g) surprisingly caused a reduction in DNA content during the larval, pupal and adult stages.

The increase in protein and RNA contents with lower doses of T4 and decrease with higher doses, indicates a biphasic nature of thyroid hormone action in silkworm fat body.

Key Words

Silkworms fat body protein nucleic acids thyroxine 

Résumé

La teneur en protéine de la matière grasse des vers à soie a augmente sous l’effect de 0.5 μg de thyroxine (T4) par gramme de poids corporel, l’augmentation étant m-e plus marquée sous l’effect de 1 μg/g 4 et 8 jours apres l’injection. Néammoins, 1 μg/g de thyroxine occasionnait deja une augmentation dès le 2 ème jour. Tous les groupes traités à la thyroxine (sauf dans le cas d’une dose de 0.25 μg/g sans effect) ont montré un niveau réduit de protéine au stade ádulte par rapport au cas témoin.

Une dose de 0.25 et 1 μg/g de thyroxine a augmenté la teneur en RNA de la matière grasse par rapport au niveau témoin observé les 2 ème, 4 ème et 8 ème jour. Mais au 13 ème jour, la teneur en RNA de ces groupes a rejoint le niveau témoin. Au cours de la période de croissance, les doses élevées de T4 (2 et 5 μg/g) ont réduit la teneur en RNA. La teneur en DNA ne fut pas modifiée par les petites doses de T4 (0,25; 0,5 et 1 μg/g) tandis que les doses plus élevées (2 et 5 μg/g) ont, d’une façon surprenante, provoqué une réduction de la teneur en DNA au couré des périodes larvaire, nymphal et adulte.

L’augmentation de la teneur en protéine et en RNA sous l’effet de petites doses de T4 et la diminution sous l’effect de doses élevées suggèrent une nature à deux phases de l’action de l’hormone thyroxidien dans la matière grasse du vers à soie.

Mots Cles

Vers à soie matière grasse protéine acide nucléique thyroxine 

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References

  1. Abalain J. H., Jego P. and Valotaire Y. (1980) Effect of 17β-estradiol on the DNA, RNA, protein contents and on the DNA, RNA polymerases in the mullerian duct of the immature female newt (Pleurodeles waltlii Michah). Gen. Comp. Endocrinol. 40, 402–408.CrossRefGoogle Scholar
  2. Ashbel R. (1935) Action of thyroid extract on the respiration of tissues of invertebrates. Nature, London, 135, 343.CrossRefGoogle Scholar
  3. Barsano C. P. and DeGroot L. J. (1983) Nuclear-cytoplasmic interrelationships. In Molecular basis of thyroid hormone action. (Edited by Oppenheimer J. H. and Samuels H. H.), pp. 139–177. Academic Press, New York.CrossRefGoogle Scholar
  4. Berg, O., Gorbman A. and Kobayashi H. (1959) The thyroid hormones in invertebrates and lower vertebrates. In Com -parative Endocrinology. (Edited by Gorbman A.), pp. 301–306. John Wiley and Sons, New York.Google Scholar
  5. Bhakthan N. M. G. and Gilbert L. I. (1968) Effects of some vertebrate hormones on lipid mobilization in the insect fat body. Gen. Comp. Endocrinol. 11, 186–197.CrossRefGoogle Scholar
  6. Chinzei Y. and Tojo S. (1972) Nucleic acid changes in the whole body and several organs of the silkworm, Bombyx mori, during metamorphosis. J. Insect Physiol. 18, 1683–1698.CrossRefGoogle Scholar
  7. Chaudhuri A. and Medda A. K. (1982) Growth stimulating effect of thyroxine on female and male silkworms, Bombyx mori L. Ind. J. Physiol. Allied Sci. 36, 62–69.Google Scholar
  8. Chaudhuri A. and Medda A. K. (1985) Effect of thyroxine on the normal pattern of variation of protein, RNA and DNA content of ovary of silkworms (Bombyx mori L.) during metamorphosis. Environ. Ecol. 3, 418–423.Google Scholar
  9. Clements A. N. (1959) Studies on the metabolism of locust fat body. J. Exp. Biol. 36, 665–675.Google Scholar
  10. Dhadialla T. S. and Wyatt G. R. (1983) Juvenile hormone-dependent vitellogenin synthesis in Locusta migratoria fat body: Inducibility related to sex and stage. Develop. Biol. 96, 436–444.CrossRefGoogle Scholar
  11. Faulkner P. and Bheemswar B. (1960) Studies on the biosynthesis of protein in silkworm, Bombyx mori. Biochem. J. 76, 71–78.CrossRefGoogle Scholar
  12. Frieden E. (1981) The dual role of thyroid hormones in vertebrate development and calorigenesis. In Metamorphosis (Edited by Gilbert L. I. and Frieden E.), 2nd, edn., pp. 545–563. Plenum Press, New York.CrossRefGoogle Scholar
  13. Gorbman, A., Clements M. and O’Brien R. (1954) Utilization of radioiodine by invertebrates with special study of several annelida and mollusca. J. Exp. Zool. 127, 75–92.CrossRefGoogle Scholar
  14. Locke M. (1981) Cell structure during insect metamorphosis. In Metamorphosis (Edited by Gilbert L. I. and Frieden E.), 2nd, edn., pp. 75–103. Plenum Press, New York.CrossRefGoogle Scholar
  15. Lowry O. H., Rosebrough N. J., Farr A. L. and Randall R. J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275.Google Scholar
  16. Majumdar A. C. and Medda A. K. (1975) Studies on the thyroxine and vitamin B12 induced changes in the life cycle of silkworms. Ind. J. Physiol. Allied Sci. 29, 1–13.Google Scholar
  17. Medda A. K. and Ray A. K. (1979) Effect of thyroxine and analogs on protein and nucleic acid contents of liver and muscle of lata fish (Ophicephalus punctatus). Gen. Comp. Endocrinol. 37, 74–80.CrossRefGoogle Scholar
  18. Medda A. K., Ray A. K., Dasgupta A. K., Majumdar A. C. and Dey C. D. (1980) Thyroid hormone actions in Magur fish and silkworms. In Thyroid Research VIII. Proceedings of the Eighth International Thyroid Congress, Sydney, Australia (Edited by Stockigt G. R. and Nagataki S.), pp. 240–243. Australian Academy of Science, Canberra.Google Scholar
  19. Munro N. H. and Fleck A. (1966) The determination of nucleic acids. In Methods of Biochemical Analysis. (Edited by Glick D.), Vol. 14, pp. 113–176. Wiley-Inter Science, New York.PubMedGoogle Scholar
  20. Ogawa K. and Tojo S. (1981) Quantitative changes of storage proteins and vitellogenin during the pupal-adult development in the silkworm, Bombyx mori (Lepidoptera: Bombycidae). Appl. ent. Zool. 16, 288–296.CrossRefGoogle Scholar
  21. Ono, S., Nagayama H. and Simura K. (1975) The occurrence and synthesis of female- and egg-specific proteins in the silkworm, Bombyx mori. Insect Biochem. 5, 319–329.CrossRefGoogle Scholar
  22. Paul A. K. and Medda A. K. (1981) Comparative study on the effects of thyroxine on the cytoplasmic area and nuclear area of liver cells of different vertebrates at different stages of life. Zool. Jb. Anal. 107, 122–135.Google Scholar
  23. Paul A. K. and Medda A. K. (1983) Comparative study on the effects of thyroxine on protein, RNA and DNA contents of liver of different vertebrates at different stages of life. Morph. Jb. 129, 239–253.Google Scholar
  24. Plisetskaya, E., Woo N. Y. S. and Murat J. C. (1983) Thyroid hormones in cyclostomes, fish and their role in regulation of intermediary metabolism. Comp. Biochem. Physiol. 2, 179–187.CrossRefGoogle Scholar
  25. Price G. M. (1973) Protein and nucleic acid metabolism in insect fat body. Biol. Rev. 48, 333–375.CrossRefGoogle Scholar
  26. Ray A. K. and Medda A. K. (1974) Effect of thyroxine on protein, RNA and DNA contents of liver and muscle of toad (Bufo melanostictus). Ind. J. Physiol. Allied Sci. 28, 147–154.Google Scholar
  27. Sass M. and Kovács J. (1974) Ultrastructural changes in the fat body cells of Mamestra brassicae (Noctuidae, Lepidoptera) during metamorphosis. Ann. Univ. Sci. Budan. Rolando Eotvos Nominatae Sect. Biol. 16, 189–205.Google Scholar
  28. Smith-Gill S. J. and Carver V. (1981) Biochemical characterization of organ differentiation and maturation. In Metamorphosis (Edited by Gilbert L. I. and Frieden E.), 2nd, edn., pp. 491–544. Plenum Press, New York.CrossRefGoogle Scholar
  29. Sridhara S. (1981) Macromolecular changes during insect metamorphosis. In Metamorphosis (Edited by Gilbert L. I. and Frieden E.), 2nd, edn., pp. 177–216. Plenum Press, New York.CrossRefGoogle Scholar
  30. Stevenson E. and Wyatt G. R. (1962) The metabolism of silkmoth tissues. I. Incorporation of Leucine into protein. Archs. Biochem. Biophys. 99, 65–71.CrossRefGoogle Scholar
  31. Tata J. R. (1974) Growth and developmental action of thyroid hormones at cellular level. In Handbook of Physiology (Edited by Greer M. A. and Solomon D. H.), Section 7, Vol. 3, pp. 469–479, American Physiological Society, Washington, D.C.Google Scholar
  32. Voitkevich A. A. and Leonova L. K. (1964) Some data on the influence of vertebrate hormones on the insect neurosecretory system. Dolk Akad. Nauk., USSR, 157, 236–239.Google Scholar
  33. Wyatt G. R. (1975) Regulation of protein and carbohydrate metabolism in insect fat body. Verh. Dtsch. Zool. Ges. 1974, 209–226.Google Scholar

Copyright information

© ICIPE 1987

Authors and Affiliations

  • Anathbandhu Chaudhuri
    • 1
  • A. K. Medda
    • 1
  1. 1.Department of Animal PhysiologyBose InstituteKankurgachi, CalcuttaIndia

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