International Journal of Tropical Insect Science

, Volume 4, Issue 4, pp 363–372 | Cite as

Factors Affecting Genotypic Susceptibility to Spotted Bollworm Earias Vittella Fab. in Cotton

  • H. C. Sharma
  • Agarwal


—The susceptibility of 23 cotton genotypes to spotted bollworm, Earias vittella, was studied under natural infestation during 1977 and 1978 rainy season at Delhi, India. There were substantial differences in bollworm damage and larval population in different genotypes. Damage in shed squares and bolls was higher than in green bolls picked up from the plant. The damage/larval population in the less susceptible cultivars (Hindiweed, XG-15, HR-26 # 8XHHG-6-IN and Sanguineum) was lower than in the susceptible check (BJR) and the trial mean. The differences in susceptibility were more pronounced at the moderate damage levels and that the damage in less susceptible cultivars increased or decreased according to the insect pressure.

There were significant differences in the gossypol, tannins, moisture, phenols and sugar content among the genotypes tested. Gossypol, tannins and moisture content of bolls and squares were negatively correlated with bollworm damage and larval population. Free phenols showed negative, but lower correlation coefficients with bollworm damage. Sugars contributed towards susceptibility while nitrogen content of bolls did not show any effect on bollworm damage. The less susceptible cultivars had higher amounts of gossypol and tannins. Some genotypes showed ovipositional non-preference because of smooth leaves.

Many factors appeared to influence the genotypic susceptibility to spotted bollworm, of which gossypol, tannins and ovipositional non-preference (due to smooth leaves) were the major factors. Gossypol and smoothness are genetically inherited and can be manipulated through crop improvement. The rating of the number and size of pigment glands on the calyx tubes and hairiness on the under surface of leaves can be used as important marker characters in breeding for spotted bollworm resistance.

Key Words

Spotted bollworm Earias vittella gossypol tannins phenols sugars plant resistance 


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  1. Association of Analytical Chemists (1975) Official Methods of Analysis. A.O.A.C. P.O. Box. 540, Benjamin Franklin Station, Washington, D.C. 20044.Google Scholar
  2. Avidov Z. and Harpaz J. (1969) Plant Pests of Israel. Israel University Press, Jerusalem.Google Scholar
  3. Badawy A. (1974) The susceptibility of certain American Upland and Sakel Cotton varieties to bollworm infestation (Lepidoptera: Arctiidae and Gelechidae). Bull. Soc. Ent. d’Egypte 58, 261–266.Google Scholar
  4. Brader L. (1969) The fauna of glandless cotton in Southern part of Chad. II. Bollworms. Cotton fibr. Trop. 24, 333–336.Google Scholar
  5. Bray H. G. and Thorpe W. V. (1955) Analysis of phenolic compounds of interest in metabolism. Meth. Biol. Anal. 1, 27–52.Google Scholar
  6. Carter C. M. and Lyman C. M. (1969) Reactions of gossypol with amino acids and other amino compounds. J. Am. Oil chem. Soc. 46, 649–653.CrossRefGoogle Scholar
  7. Chan B. G. and Waiss Jr. A. C. (1978) Condensed tannin, and antibiotic chemical from Gossypium hirsutum. J. Insect Physiol. 24, 113–118.CrossRefGoogle Scholar
  8. Chang G. S., Meng H. L. and Bao J. A. (1963) The status of the spotted bollworms, Earias fabia (Stoll) and Earias insulana (Boisd.) and the changes of cultural system in Lukang Poashan District, Yunnan Province. Acta ento. Sìnica 12, 28.Google Scholar
  9. Clark E. W. and Lukefahr M. J. (1966) A partial analysis of cotton extra floral nectar and its approximation as a nutritional medium for adult pink bollworms. J. econ. Ent. 49, 875–876.CrossRefGoogle Scholar
  10. Deshpande B. P. and Nandkarny E. T. (1936) The spotted bollworms of cotton, Earias fabia Stoll and Earias insulana Boisd. in South Gujarat, Bombay Presidency. I.C.A.R. Sci. Monogr. 10, 1–208.Google Scholar
  11. Eagle E., Hall C. M., Castillan L. E. and Miller C. B. (1950) Effect of fractionation and treatment on the acute oral toxicity, and gossypol and gossypurin content of cotton seed pigment glands. J. Am. Oil chem. Soc. 27, 300–303.CrossRefGoogle Scholar
  12. Elliger C. A., Chan B. G. and Waiss Jr. A. C. (1978) Relative toxicity of minor cotton terpenoids compared to gossypol. J. econ. Ent. 71, 161–164.CrossRefGoogle Scholar
  13. Fletcher T. B. (1914) Some South Indian Insects. Madras Govt. Press.Google Scholar
  14. Gray J. R., Marby T. J., Bell A. A. and Stipanovic R. D. (1976) Para-hemigossypolone; a sesquiterpenoid aldehyde from Gossypium hirsutum. J. Chem. Soc. Commun. 109–110.Google Scholar
  15. Hussain M. A. and Khan M. H. (1940) Studies on Platyedra gossypiella Saunders in Punjab. IX. Relative incidence on exotic and indigenous varieties of cotton. Indian J. Ent. 40, 245–257.Google Scholar
  16. Lee J. A. (1971) Some problems in breeding smooth leaved cottons. Crop Science 11, 448–450.CrossRefGoogle Scholar
  17. Lee J. A., Cockerhan C. C. and Smith F. H. (1968) The inheritance of gossypol level in Gossypium. I. Additive, dominance, epistatic and maternal effects associated with seed gossypol in two varieties of Gossypium hirsutum. Genetics 59, 285–298.PubMedPubMedCentralGoogle Scholar
  18. Lincolon C. and Waddle B. A. (1966) Insect resistance of Frego type of cotton. Arkans Fam. Res. 15, 4–5.Google Scholar
  19. Lincolon C., Dean G., Waddle B. A., Yearian W. C., Philips J. R. and Roberts L. (1971) Resistance of Frego type cotton to bollworm and boll weevil. J. econ. Ent. 64, 1326–1327.CrossRefGoogle Scholar
  20. Lukefahr M. J. and Houghtaling J. E. (1969) Resistance of cotton strains with high gossypol content to Heliothis spp. J. econ. Ent. 62, 588–591.CrossRefGoogle Scholar
  21. Lukefahr M. J., Martin D. F. and Meyer J. R. (1965) Plant resistance to five Lepidoptera attacking cotton. J. econ. Ent. 58, 516–518.CrossRefGoogle Scholar
  22. Lukefahr M. J., Nobel C. W. and Houghtaling J. E. (1966a) Growth and infestation of bollworms and other insects on glanded and glandless strains of cotton. J econ. Ent. 59, 817–820.CrossRefGoogle Scholar
  23. Lukefahr M. J., Crown C. B., Pfrimmer T. R. and Noble C. W. (1966b) Resistance of experimental cotton strain 1514 to bollworm and cotton fleahopper. J. econ. Ent. 59, 393–395.CrossRefGoogle Scholar
  24. Lukefahr M. J., Shaver T. N., Cruhm D. G. and Houghtaling J. E. (1974) Location, transference and recovery of a Heliothis growth inhibition factor present in three Gossypium hirsutum race stocks. Beltwide cotton production Research Conference Proceedings, 1974.Google Scholar
  25. Lukefahr M. J., Houghtaling J. E. and Cruhm D. G. (1975) Suppression of Heliothis spp. with cotton containing combinations of resistant characters. J. econ. Ent. 68, 743–746.CrossRefGoogle Scholar
  26. Lyman C. M., Baliga B. P. and Margaret S. W. (1959) Reaction of proteins with gossypol. Archs Biochem. Biophys. 84, 486–497.CrossRefGoogle Scholar
  27. Meisner J., Ishaaya I., Asher K. R. S. and Arom M. Z. (1978) Gossypol inhibits protease and amylase activity of Spodoptera littoralis larvae. Ann. ent. Soc. Am. 71, 5–8.CrossRefGoogle Scholar
  28. Meisner J., Kehat M. and Asher K. R. S. (1977) The effect of gossypol on the larvae of spiny bollworm, Earias insulana. Ent. exp. Appl. 22, 301–303.CrossRefGoogle Scholar
  29. Meyer J. R. (1957) Origin and inheritance of D2 smoothness in upland cotton. J. Hered. 45, 245–250.Google Scholar
  30. Nangpal H. D. (1948) Insect Pests of Cotton in India. Indian Central Cotton Committee; Bombay.Google Scholar
  31. Oliver B. F., Maxwell F. G. and Jenkins J. N. (1971) Growth of bollworm on glanded and glandless cotton. J. econ. Ent. 63, 1328–1329.CrossRefGoogle Scholar
  32. Plummer D. T. (1974) An Introduction to Practical Biochemistry. Tata McGraw Hill Pub. Co. Ltd. New Delhi.Google Scholar
  33. Reed W. (1974a) Populations and host plant preferences of Earias spp. (Lepidoptera:Noctuidae) in East Africa. Bull. Ent. Res. 64, 33–44.CrossRefGoogle Scholar
  34. Reed W. (1974b) Selection of cotton varieties for resistance to insect pests in Uganda. Cotton Grow. Rev. 51, 106–123.Google Scholar
  35. Rhyne C. L. and Smith F. H. (1965) Genetic aspects of gossypol content of leaves and flower buds of Gossypium. Crop. Sci. 5, 410–421.Google Scholar
  36. Sharma H. C. and Agarwal R. A. (1982a) Resistance mechanisms in some cotton genotypes to shoot damage by spotted bollwrom (Earias vittella Fab.). Protection Ecology (accepted).Google Scholar
  37. Sharma H. C. and Agarwal R. A. (1982b) Consumption and utilization of bolls of different cotton genotypes by larvae of Earias vittella Fab. and effect of gossypol and tannins on food utilization. Zeitschrift Ange. Zool. 68, 13–38.Google Scholar
  38. Sharma H. C. and Agarwal R. A. (1982c) Effect of some antibiotic compounds in Gossypium on the postembryonic development of spotted bollworm (Earias vittella Fab.). Ent. exp. Appl. 31, 225–228.CrossRefGoogle Scholar
  39. Sharma H. C., Agarwal R. A. and Munshi Singh (1982). Effect of some antibiotic compounds in cotton on post-embryonic development of spotted bollworm (Earias vittella F.) and the mechanism of resistance in Gossypium arboreum. Proc. Indian Acad. Sci. 91, 67–77.CrossRefGoogle Scholar
  40. Smith F. H. (1967) Determination of gossypol in leaves and flower buds of Gossypium. J. Am. Oil. chem. Soc. 44, 267–269.CrossRefGoogle Scholar
  41. Sohi G. S. (1964) Pests of cotton. In Entomology in India (Ed. N. C. Pant), pp. 111–148. Entomological Society of India.Google Scholar
  42. Somoygi M. (1952) Notes on sugar determination. J. biol. Chem. 195, 19–23.Google Scholar
  43. Stipanovic R. D., Bell A. A. and Lukefahr M. J. (1976). Natural insecticides of cotton, structural analysis and toxicology of Heliocides. 172nd Nat. Mfg. Amer. Chem. Soc. Aug 29-Sep 3, 1976 San Francisco, U.S.A. Pest. Div. Abs. No. 78.Google Scholar
  44. Stipanovic R. D., Bell A. A., O’Brien D. H. and Lukefahr M. J. (1977) Heliocide-H2: an insecticidal sesquiterpenoid from cotton (Gossypium). Tetrahedron Lett. 567–70.Google Scholar
  45. Tanksley T. D., Neumann H., Layman C. M., Pace C. N. and Prescott J. M. (1970). Inhibition of pepsinogen activation. J. biol. Chem. 245, 6451–6456.Google Scholar
  46. Walker R. L. (1952) Spiny bollworm of cotton in Iraq. FAO. Plant Protection Bulletin. 1, 42.Google Scholar
  47. Wilson F. D. and Smith J. N. (1976) Some genetic relationships between gland density and gossypol content in Gossypium hirsutum L. Crop. Sci. 16, 830–832.CrossRefGoogle Scholar
  48. Yang H. C. and Davis D. D. (1976) Variations in gossypol concentrations of flower buds of cotton. Crop. Sci. 16, 485–488.CrossRefGoogle Scholar

Copyright information

© ICIPE 1983

Authors and Affiliations

  • H. C. Sharma
    • 1
  • Agarwal
    • 1
  1. 1.ICRISATPatancheruIndia

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