International Journal of Tropical Insect Science

, Volume 4, Issue 4, pp 357–362 | Cite as

A General Purpose Laboratory Diet Mixture for Rearing Insects Une Nourriture

  • Pritam Singh


The composition and preparation of a general purpose artificial diet for rearing insects is described. Forty-one species were reared for one generation or more, and 56 species were partially reared or maintained on this diet. The advantages and applications of using a single diet for multiple-species rearing are described.

The diet is economical (NZ $2.37 per kg) for general laboratory rearing. In freeze-dried form it can be used for bioassays of pathogens, pesticides, insect growth regulators, plant resistant chemicals and other toxins, and has a potential market in the development of insect rearing kitsets for educational purposes.

Key Words

Artificial diet rearing insects 

Mélangée De Dessein Général Pour L’élevage Des Insectes Dans Le Laboratoire


L’auteur rapport la composition et la préparation d’un milieu artificiel pour l’élevage de beaucoup des insectes. Quarante et un espèces ont pu être élevées pendant une ou plusieurs générations et ont été élevées partialement ou maintenues sur ce milieu. Les avantages et les applications d’employer un seul milieu pour l’élevage d’espèces multiple sont décrites. Le milieu est économique (FF 11.58 kg−1) pour l’élevage général dans le laboratoire et il peut être accumulé pendant plus que deux années sous la forme lyophilisée.

Ce peut être employé à faire l’essai biologique des pathogènes, des insecticides, des régulateurs de la croissance des insects, des chemiques plante-résistantes et des autres toxines, et il a un marché potentiel pour le développement des jeux de construction pour l’élevage des insectes dans les buts d’enseignement.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Altman P. L. and Dittmer D. S. (Eds). (1968) Nutrient requirements and utilization; insects, pp. 148–163; synthetic diets: insects, pp. 164–167. In Metabolism. Federation of American Societies of Experimental Biology, Bethesda, Maryland.Google Scholar
  2. Deitz L. L., Van Duyn J. W., Bradley J. R. Jr, Rabb R. L., Brooks W. M. and Stinner R. E. (1976) A guide to the identification and biology of soybean arthropods in North Carolina. North Carolina Agricul. Expt Stn Tech. Bull. No. 238.Google Scholar
  3. Galford J. R. (1969) Artificial rearing of 10 species of wood boring insects. U.S. For. Serv. Res. Note NE 102.Google Scholar
  4. Gardiner L. M. (1970) Rearing wood-boring beetles (Cerambycidae) on artificial diet. Can. Ent. 102, 113–117.CrossRefGoogle Scholar
  5. Goujet R. and Guilbot R. (1979) Alimentation artificielle de plusieurs Lépidoptères ravageurs du feuillage de Pommier. Ann. Zool. Ecol. Anim. 11, 119–124. (In French with English summary).Google Scholar
  6. Grisdale D. G. (1963) Rearing insects on artificial diet. Interim Research report of the Insect Pathology Research Institute, Sault Ste Marie, Ontario, Canada.Google Scholar
  7. Hansen L. O. and Zethner O. (1979) Techniques for rearing 26 species of Noctuidae Lepidoptera on an artificial diet. Kgl. Vet. og Landbohojsk Arsskr. 84–97.Google Scholar
  8. Herger P. and Lampel, G. (1975) Zuchtversuche mit mono-bis polyphagen Rörenblattlaus—Arten auf Künstlichem Nährmedium (Homoptera: Aphidina: Aphididae). Eni. Germ. 1, 258–278. (In German with English summary.)Google Scholar
  9. Hinks C. F. and Byers J. R. (1976) Biosystematics of the genus Euxoa (Lepidoptera:Noctuidae). V. Rearing procedures and life cycle of 36 species. Can. Ent. 108, 1345–1357.CrossRefGoogle Scholar
  10. Kardatzke J. T. (1979) Juvenile development of snow melt Aedes. Mosq. News 39, 527–531.Google Scholar
  11. Kardatzke J. T. (1980) Effect of sodium chloride on larval snow-melt Aedes (Diptera: Culicidae). Mosq. News 40, 153–160.Google Scholar
  12. Khalaf K. T. (1979) A list of larvae sustained on wheatgerm diet. J. Lepid. Soc. 33, 196–197.Google Scholar
  13. Klein J. A. and Beck S. D. (1979) A meridie diet for Trogoderma glabrum and other dermestid beetles. Ann. Ent. Soc. Am. 72, 398–404.CrossRefGoogle Scholar
  14. Kurstak E., Maramorsch K., Diibendorfer A. (1980) Invertebrate Systems In Vitro. Elsevier/North Holland.Google Scholar
  15. Luckey T. D. (1954) A single diet for all living organisms. Science, N.Y. 120, 396–398.CrossRefGoogle Scholar
  16. Luckey T. D. (1977) Comparative nutrition. (Ed. Recheigl, M. Jr). In Handbook Series in Nutrition and Food pp. 3–19, Section D, Vol. 1. CRC Press, Cleveland, Ohio.Google Scholar
  17. Mitsuhashi J. (1972) Invertebrate Tissue Culture, Vol. 2. (Ed. Vago C.). Academic Press, New York.Google Scholar
  18. Morton A. C. (1981) Rearing butterflies on artificial diets. J. Res. Lepid. 18, 221–227.Google Scholar
  19. Okada I. and Niijima K. (1977) Artificial rearing of lady beetles—the present status and problems. Kontyu to Shizen 12, 20–25. (In Japanese)Google Scholar
  20. Paul J. (1970) Cell and Tissue Cultures, 4th edn, Livingstone, Edinburgh, U.K.Google Scholar
  21. Poitout S. and Bues R. (1970) Élevage de plusieurs espèces de Lépidoptères Noctuidae sur milieu artificial riche et sur milieu artificiel simplifié. Ann. Zool. Ecol. Anim. 2, 79–91. (In French with English summary)Google Scholar
  22. Poitout S. and Bues R. (1974) Élevage de chenilles de vingt-huit espèces de Lépidoptères Noctuidae et de deux espèces d’Arctidae sur milieu artificiel simple. Particularités de l’élevage selon les espèces. Ann. Zool. Ecol. Anim. 6, 431–441.Google Scholar
  23. Recheigl M. Jr (1977) Handbook Series in Nutrition and Food. Section G. Vol. II. CRC Press, Cleveland, Ohio.Google Scholar
  24. Singh P. (1974) Improvements in or relating to artificial dietary mixture for insects. NZ Patent No. 174449 (complete specification filed 2nd June 1975).Google Scholar
  25. Singh P. (1977) Artificial Diets for Insects, Mites and Spiders. Plenum Press, New York.CrossRefGoogle Scholar
  26. Singh P. and Mittler T. E. A chemically defined diet for rearing aphids (in preparation).Google Scholar
  27. Singh P., Johnson, J. B. and Hagen, K. S. Development of a practical alternative to the use of fresh or live prey in culturing Leis ( = Harmonia) conformis Boisduval (Cole-optera:Coccinellidae) (in preparation).Google Scholar
  28. Singh P. and Surrey M. R. (1980) A plastic container for rearing insects on artificial diets. N.Z. J. Zool. 7, 441–442.CrossRefGoogle Scholar
  29. Smirnoff W. A. (1958) An artificial diet for rearing coccinellid beetles. Can. Ent. 90, 563–565.CrossRefGoogle Scholar
  30. Smith B. C. (1965) Effects of food on the longevity, fecundity, and development of adult coccinellids (Coleoptera: Coccinellidae). Can. Ent. 97, 910–919.CrossRefGoogle Scholar
  31. Steffan W. A. (1966) A generic revision of the family Sciaridae (Diptera) of America north of Mexico. Univ. Calif. Berkeley Pubi. Ent. 44, 1–77.Google Scholar

Copyright information

© ICIPE 1983

Authors and Affiliations

  • Pritam Singh
    • 1
  1. 1.Department of Scientific and Industrial ResearchEntomology DivisionAucklandNew Zealand

Personalised recommendations