International Journal of Tropical Insect Science

, Volume 4, Issue 3, pp 227–236 | Cite as

Histochemical and Ultrastructural Studies of the Male Accessory Reproductive Glands and Spermatophore of the Tsetse, Glossina Morsitans Morsitans Westwood

  • Thomas R. Odhiambo
  • Elizabeth D. Kokwaro
  • Lina M. Sequeira


A pair of accessory reproductive glands (ARGs) containing a variety of secretory products forms a part of the male reproductive system of the tsetse, Glossina morsitans morsitans Westwood. All of the ARG cells have an extensive rough endoplasmic reticulum which is indicative of the production of proteinaceous substances to be secreted. Histochemically, the secretory products in the lumen of the ARG contain a neutral mucopolysaccharide-protein complex, and are products of several morphological types. The latter are probably stages of maturation of the secretory materials that eventually become the wall of the spermatophore, a highly organized structure which acts as a temporary container for the spermatoza during their transfer to the spermathecae, and is histochemically composed of a mixture of carbohydrates and proteins. On grounds of histochemical and ultrastructural similarities, we suggest that the spermatophore of G. morsitans is derived, at least partially, from the male ARGs.

Key Words

Accessory reproductive glands spermatophore tsetse Glossina morsitans morsitans ultra-structural studies mucopolysaccharide-protein complex secretory materials 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Culling C. F. A. (1974) Handbook of Histopathological Techniques. Butterworths, London.Google Scholar
  2. De Loof and Lagasse A. (1972) The ultrastructure of the male accessory reproductive glands of the Colorado beetle. Z. Zellforsch. mikrosk. 130, 545–552.CrossRefGoogle Scholar
  3. Drury R. A. B. and Wallington E. A. (1967) Histological Techniques. Oxford University Press, London.Google Scholar
  4. Engelmann F. (1970) The Physiology of Insect Reproduction. Pergamon Press Ltd., Oxford.Google Scholar
  5. Feldman-Muhsam B., Borut S., Saliternic-Givant S. and Eden C. (1973) On evacuation of sperm from the spermatophore of the tick Ornithodoros savignyi. J. Insect Physiol. 19, 951–962.CrossRefGoogle Scholar
  6. Gadzama N. M. and Happ G. M. (1974) The structure and evacuation of the spermatophore of Tenebrio molitor L. (Coleoptera—Tenebrionidae). Tissue & Cell 6, 95–108.CrossRefGoogle Scholar
  7. Khalifa A. (1949) The mechanism of insemination and the mode of action of the spermatophore in Gryllus domesticus. Q. J. Microsc. Sci. 90, 281–292.Google Scholar
  8. Kokwaro E. D. and Odhiambo T. R. (1981) Spermatophore of the tsetse Glossina morsitans morsitans Westw. An ultrastructural study. Insect Sci. Application 1, 185–190.Google Scholar
  9. Leopold R. A. (1970) Cytological and cytochemical studies on the ejaculatory duct and accessory secretions in Musca domestica. J. Insect Physiol. 16, 1859–1972.CrossRefGoogle Scholar
  10. Leopold R. A. (1976) The role of accessory glands in insect reproduction. A. Rev. Ent. 21, 199–221.CrossRefGoogle Scholar
  11. Odhiambo T. R. (1969a) The architecture of the accessory reproductive glands of the desert locust—1. Types of glands and their secretions. Tissue & Cell 1, 155–182.CrossRefGoogle Scholar
  12. Odhiambo T. R. (1969b) The architecture of the accessory reproductive glands of the desert locust—IV. Fine structure of the glandular epithelium. Phil. Trans. R. Soc. 256, 85–114.CrossRefGoogle Scholar
  13. Pearse A. G. E. (1968) Histochemistry: Theoretical and Applied, Vol. 1.J & A. Churchill, London.Google Scholar
  14. Pollock J. N. (1970) Sperm transfer by spermatophore in Glossina austeni Newstead. Nature, Lond. 225, 1063–1064.CrossRefGoogle Scholar
  15. Pollock J. N. (1974) Male accessory secretions, their use and replenishment in Glossina (Diptera: Glossinidae). Bull. ent. Res. 64, 533–539.CrossRefGoogle Scholar
  16. Ramasamy M. S. (1981) Influence of feeding and hormonal factors on sexual maturation in male Glossina morsitans morsitans Westw. (Diptera: Glossinidae) Insect Sci. Application 1, 273–280.Google Scholar
  17. Riemann J. G. and Thorson B. J. (1979) Ultrastructure of the accessory glands of the Mediterranean flour moth, Anagasta kühniella (Zeller). J. Morph. 159, 355–392.CrossRefGoogle Scholar

Copyright information

© ICIPE 1983

Authors and Affiliations

  • Thomas R. Odhiambo
    • 1
  • Elizabeth D. Kokwaro
    • 1
  • Lina M. Sequeira
    • 1
  1. 1.The International Centre of Insect Physiology and Ecology (ICIPE)NairobiKenya

Personalised recommendations