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International Journal of Tropical Insect Science

, Volume 2, Issue 3, pp 181–187 | Cite as

The Isolation and Identification of the Cuticular Sex-Stimulant Pheromone of the Tsetse Glossina Pallidipes Austen (Diptera: Glossinidae)

  • P. G. McDowell
  • D. L. Whitehead
  • M. F. B. Chaudhury
  • W. F. Snow
Article

Abstract

The sex-stimulant contact pheromone present in the cuticle of female tsetse Glossina pallidipes Austen has been extracted with hexane, separated by preparative gas chromatography and identified by mass spectrometry. The aphrodisiac, consisting of 13,17-dimethylpentatriacontane and 15,19-dimethylpentatriacontane, is present in cuticular extracts of 1-week-old females and, in lower amounts, in teneral females but it is almost absent in males of all ages. Full copulatory responses to hexane-washed dead males treated with the two dimethylpentatriacontane isomers were induced in 10–20-day-old adult male G. pallidipes. The practical uses to which this discovery can now be put—towards the mass-rearing and control of G. pallidipes—are discussed in detail.

Key Words

Tsetse Glossina pallidipes pheromone sex-stimulant, vdimethylpentatriacontanes autochemosterilization 

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References

  1. Baker R., Bradshaw J. W. S., Evans D. A., Higgs M. D. and Wadhams J. (1976) An efficient all-glass splitter and trapping system for gas chromatography. J. Chromat. Sci. 14, 425–427.CrossRefGoogle Scholar
  2. Blomquist G. J., Blailock T. T., Scheetz R. W. and Jackson L. L. (1976) Cuticular lipids of insects — VII. Cuticular hydrocarbons of the crickets Acheta domesticus, Gryllus Pennsylvania and Nemobius fasciatus. Comp. Biochem. Physiol. 54B, 381–386.Google Scholar
  3. Carlson D. A., Langley P. A. and Huyton P. (1978) Sex pheromone of the tsetse fly: isolation, identification, and synthesis of contact aphrodisiacs. Science 201, 750–753.CrossRefGoogle Scholar
  4. Challier A. (1965) Amélioration de la méthode de détermination de l’âge physiologique des glossines. Etudes faites sur Glossina palpalis gambiensis Vanderplank, 1949. Bull. Soc. Path. exot. 58, 250–259.PubMedGoogle Scholar
  5. Challier A. (1973) Ecologie de Glossina palpalis gambiensis Vanderplank, 1949 (Diptera: Muscidae) en savane d’Afrique occidentale. Mém. ORSTOM, Paris 64, 274.Google Scholar
  6. Challier A. (1977) Trapping technology. In Tsetse, The Future for Biological Methods in Integrated Control (Ed. by Laird M.), IDRC-077e, Ottawa.Google Scholar
  7. Challier A. and Gouteux J.-P. (1978) Enquêtes entomologiques dan le foyer de maladie du Sommeil de Vavous — Republiques de Côte d’Ivoire (janmar 1978). II. Possibilitiés et essais de lutte en zone forestière contre Glossina palpalis palpalis (R-D). Rapport OCCGE-Centre Muraz, number 20/ENT. 78.Google Scholar
  8. Challier A. and Laveissiere C. (1973) Un nouveau piège pour la capture des glossines (Glossina:Diptera, Muscidae): description et essais sur le terrain. Cah. O.R.S.T.O.M. Sér. Ent. med. Parasit. 11, 252–262.Google Scholar
  9. Harley J. M. N. (1966) Studies on age and trypanosome infection rate in females of Glossina pallidires Aust., G. palpalis fuscipes Newst, and G. brevipalpis Newst, in Uganda. Bull. ent. Res. 57, 23–37.CrossRefGoogle Scholar
  10. Howard R. W., McDaniel C. A., Nelson D. R. and Blomquist G. J. (1980) Chemical ionisation mass spectrometry. Application to insect derived cuticular alkanes. J. chem. Ecol. 6, 609–623.CrossRefGoogle Scholar
  11. Huyton P. M., Langley P. A., Carlson D. A. and Coates T. W. (1980a) The role of sex pheromones in initiation of copulatory behaviour by male tsetse flies, Glossina morsitans morsitans. Physiol. Ent. 5, 243–252.CrossRefGoogle Scholar
  12. Huyton P. M., Langley P. A., Carlson D. A. and Schwarz M. (1980b) Specificity of contact sex pheromones in tsetse flies Glossina spp. Physiol. Ent. 5, 253–264.CrossRefGoogle Scholar
  13. Jaenson T. G. T. (1980) Mating behaviour of females of Glossina pallidipes Austen (Diptera:Glossinidae). Bull. ent. Res. 70, 49–60.CrossRefGoogle Scholar
  14. Jordan A. M. (1958) The mating behaviour of females of Glossina palpalis (R-D) in captivity. Bull. ent. Res. 49, 35–43.CrossRefGoogle Scholar
  15. Kovats E. (1965) Gas chromatographic characterization of organic substances and the retention index system. In Advances in Chromatography (Ed. by Giddings J. C.), Vol. 1, Chap. 7, pp. 229–247.Google Scholar
  16. Langley P. A. (1977) Physiology of tsetse flies (Glossina spp.) (Diptera: Glossinidae): a review. Bull. ent. Res. 67, 527–574.CrossRefGoogle Scholar
  17. Langley P. A., Carlson D. A. Huyton P. M. and Coates T. W. (1979) Prospects for use of specific sex pheromones for tsetse control. 16th Meeting of International Scientific Council for Trypanosomiasis Research and Control, Yaounde, Cameroun.Google Scholar
  18. Langley P. A., Pimley R. W. and Carlson D. A. (1975) Sex recognition pheromone in tsetse fly Glossina morsitans. Nature, Lond. 254, 51–53.CrossRefGoogle Scholar
  19. Nash T. A. M., Jordan A. M. and Trewern M. A. (1971) Mass rearing of tsetse flies (Glossina spp.): present advances. In Sterility Principle for Insect Control or Eradication (publication No. sII/PUB/265), pp. 99–110. International Atomic Energy Agency, Vienna.Google Scholar
  20. Okiwelu S. N. (1977) Insemination, pregnancy and suspected abortion rates in a natural population of Glossina morsitans morsituns (Diptera: Glossinidae) in the Republic of Zambia. J. med. Ent. 14, 19–23.CrossRefGoogle Scholar
  21. Rogers A. (1972) Studies on the mating of Glossina palldipes Austen. I. The age at mating. Ann. trap. Med. Parasit. 66, 515–523.CrossRefGoogle Scholar
  22. Saunders D. S. (1960) Ovulation cycle in Glossina morsitans Westwood (Diptera:Muscidae) and a possible method of age determination for female tsetse flies by the examination of their ovaries. Trans. R. ent. Soc. Lond. 112, 221–238.CrossRefGoogle Scholar
  23. Saunders D. S. (1962) Age determination for female tsetse flies and the age compositions of samples of Glossina pallidipes (Aust., G. palpalis fuscipes Newst and G. brevi-palpis Newst. Bull. ent. Res. 53, 579–595.CrossRefGoogle Scholar
  24. Vale G. A. and Hargrove J. W. (1975) Field attraction of tsetse flies (Diptera:Glossinidae) to ox odour; the effect of dose. Proc. Trans. Rhod. scient. Ass. 56, 46–50.Google Scholar
  25. van Etten J. (1981) A comparison of the performance of laboratory colonies of Glossina pallidipes Austen from two allotropic populations in Kenya. Insect Sci. Applic. 1, 177–183.Google Scholar
  26. Whitehead D. L. (1981) The effect of phytosterols on tsetse reproduction. Insect Sci. Applic. 1, 281–288.Google Scholar

Copyright information

© ICIPE 1981

Authors and Affiliations

  • P. G. McDowell
    • 1
  • D. L. Whitehead
    • 1
  • M. F. B. Chaudhury
    • 1
  • W. F. Snow
    • 2
  1. 1.International Centre of Insect Physiology and Ecology (ICIPE)NairobiKenya
  2. 2.ICIPE Muhaka Field StationMombasaKenya

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