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Noninvasive genetic assessment provides evidence of extensive gene flow and possible high movement ability in the African golden wolf

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Abstract

The African golden wolf, Canis anthus, is a newly recognized medium-sized canid species from North Africa, which has remained poorly studied to date. We applied genetic methods for individual identification of non-invasively collected samples (n = 93) of the African golden wolf in Tunisia, assessing their genetic diversity and structure. The mitochondrial control region exhibited high haplotype diversity (Hd = 0.907 ± 0.018) with 15 haplotypes detected among 28 individuals. Similarly, a set of 15 microsatel-lite loci revealed high genetic diversity at the nuclear level (expected heterozygosity = 0.83±0.04; average number of alleles = 8.30 ± 0.99). The Bayesian model-based clustering method implemented in STRUCTURE did not reveal population structure in African golden wolves within Tunisia. This result was corroborated by the Discriminant Analysis of Principal Components where no clear clusters were observed. Based on seven individuals that were found multiple times among samples, we estimated straight distance movements up to 230 km. Our results provide movement lengths for the species and no evidence for biogeographical structure of genetic diversity within Tunisian golden wolves, probably related to the high dispersal ability of the species, facilitating high gene flow. The evaluation of population diversity and the first information on movement ability provided in this study should be considered as baseline information in the development of a management plan for African golden wolf in Tunisia.

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Authors and Affiliations

  1. Département de Biologie, Faculté des Sciences de Tunis, Université de Tunis El- Manar, 2092, Tunisia

    Yamna Karssene & Said Nouira

  2. Laboratoire d’Elevage et de la Faune Sauvage, Institut des Régions Arides de Médenine, 4119, Médenine, Tunisia

    Yamna Karssene & Mohsen Chammem

  3. Senckenberg Research Institute and Natural History Museum Frankfurt, Clamecystraße. 12, 63571, Gelnhausen, Germany

    Carsten Nowak, Violeta Muñoz-Fuentes & Berardino Cocchiararo

  4. Society of North African Big Carnivores Stichting, Drabstraat 288, BE-2640, Mortsel, Belgium

    Koen de Smet, Dick Klees & Peter Van Der Leer

  5. CIBIO/InBio - Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus de Vairão, 4485-661, Vairão, Portugal

    Diana Castro, Susana Lopes & Raquel Godinho

  6. Laboratory of water conservatory management soil and forest, Faculty of Nature and Life Sciences, University ofTlemcen, Tlemcen, 13000, Algeria

    Ahmed Eddine & Raquel Godinho

  7. Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007, Porto, Portugal

    Raquel Godinho

  8. Department of Zoology, University of Johannesburg, P.O. Box 534, Auckland Park, 2006, South Africa

    Raquel Godinho

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  1. Yamna Karssene
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  2. Mohsen Chammem
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  3. Carsten Nowak
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  12. Said Nouira
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Correspondence to Yamna Karssene or Raquel Godinho.

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Karssene, Y., Chammem, M., Nowak, C. et al. Noninvasive genetic assessment provides evidence of extensive gene flow and possible high movement ability in the African golden wolf. Mamm Biol 92, 94–101 (2018). https://doi.org/10.1016/j.mambio.2018.05.002

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