Cardiac glycosides from Cascabela thevetioides by HPLC-MS analysis


The present work investigates the chemical composition of seeds of Cascabela thevetioides (Kunth) Lip-pold, an ornamental shrub of México. Six thevetia cardiac glycosides or thevetosides (thevetin A, B, and C, acetylthevetin A, B and C) were identified from the methanol extract of seeds of C. thevetioides by High-Performance Liquid Chromatography-Mass Spectrometry and by comparison of mass spectral fragmentation patterns. Enzymatic hydrolysis of a sample of thevetosides from methanol extract of seeds and subsequent High-Performance Liquid Chromatography-Mass Spectrometry analysis yielded the monoglycosides neriifolin, acetylneriifolin and acetylperuvoside, previously reported for this plant. For the fisrt time thevetin A, B and C, and acetylthevetin A, B and C are reported as components of seeds of C. thevetioides.


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Correspondence to José Luis Balderas-López.

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Author’s contributions

SB and EP-R contributed in running the laboratory work, extractions and acquisition of the data. JCT-C and JLB-L contributed in analysis and interpretation of data. JLB-L and AN drafted the paper.

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Balderas-López, J.L., Barbonetti, S., Pineda-Rosas, E.L. et al. Cardiac glycosides from Cascabela thevetioides by HPLC-MS analysis. Rev. Bras. Farmacogn. 29, 441–444 (2019).

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  • Cardiac glycosides
  • High-performance liquid chromatography
  • Mass spectrometry
  • Thevetosides