A valepotriate-enriched fraction from Valeriana glechomifolia Meyer inhibits leukocytes migration and nociception in formalin test in rodents

Abstract

Previous pre-clinical studies demonstrated that a valepotriates enriched fraction from Valeriana glechomifolia F.G. Mey., Caprifoliaceae, was effective against lipopolysaccharide from Escherichia coli (LPS)-induced sickness behavior as well as significantly decreased the cortical expression of pro inflammatory cytokines interleukin-1β and tumor necrosis factor-α. Other studies revealed anti-inflammatory properties of V. wallichii and V. amurensis. These findings open up new perspectives for Valeriana genus pharmacology, once it has been commonly associated to sedative and anxiolytic properties. The aim of this study was to investigate the antichemotactic, antinociptive and anti-inflammatory activities of a valepotriate-enriched fraction obtained from aerial and subterranean parts of V. glechomifolia submitted to supercritical CO2 extraction. The biological activities were assessed by means of formalin test in CF1 mice and Wistar rat’s leukocytes migration assay (modified Boyden chamber method). Valepotriate-enriched fraction (1, 10 and 30 mg/kg, p.o.) inhibited the nociceptive behavior in the late phase of the formalin test in a dose dependent manner. The effect of the valepotriate-enriched fraction highest dose was comparable with that of diclofenac 50 mg/kg (p.o.). Valepotriate-enriched fraction (0.1–1 μg/ml) inhibited the leukocyte migration induced by lipopolysaccharide from Escherichia coli in a concentration dependent manner. This antichemotatic effect was comparable with that of indomethacin (0.1–1 μg/ml) and better than diclofenac (1 μg/ml) effect. This study demonstrated for the first time that a valepotriate-enriched fraction obtained from V. glechomifolia display a peripheral anti-inflammatory like activity.

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Correspondence to Stela M. K. Rates.

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Authors’ contributions

Study concept and design: TMA, LJD, MAA, LGM, SMKR. Acquisition of data: TMA, LJD, EC, RMFV. Analysis and interpretation of data: TMA, LJD, MAA, EC, RMFV, GLVP, LGM, SMKR. Drafting of the manuscript: TMA, LJD, LGM, MAA, SMKR. Study supervision: LGM, SMKR. All authors have read and approved the final version of the manuscript.

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de Almeida, T.M., Danielli, L.J., Apel, M.A. et al. A valepotriate-enriched fraction from Valeriana glechomifolia Meyer inhibits leukocytes migration and nociception in formalin test in rodents. Rev. Bras. Farmacogn. 29, 477–482 (2019). https://doi.org/10.1016/j.bjp.2019.02.004

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Keywords

  • Anti-inflammatory
  • Formalin
  • Neutrophil chemotaxis
  • Valepotriates
  • Valerianaceae