Evaluation of the immunomodulatory effect against splenocytes of Balb/c mice of biflorin obtained from Capraria biflora by a new isolation method

Abstract

Biflorin (6,9-dimethyl-3-(4-methylpent-3-en-1-yl) benzo[de]chromene-7,8-dione) is a promising substance that has been increasingly studied in the past decades due to its diverse pharmacological properties (i.e. antitumor, antioxidant, antiinflamatory, antimicrobial activity etc.). Aiming the comprehension of its antitumoral activity we investigated the cell proliferation and cytotoxicity habilities of biflorin against mice splenocytes Balb/c. Biflorin was able to stimulate mice splenocytes Balb/c in 48 h of incubation at a concentration of 20.2 μM. Its immunostimulation promoted the production of cytokines such as: TNF-α, IFN-γ, IL-2, IL-6 and IL-17, inducing the immune profile toward a Th1 response. Moreover, an original method which led to an excellent yield with less processing time compared to the methods described in the literature was developed to obtain biflorin, from sawdust of Capraria biflora L., Scrophulariaceae. This method shows a great potential of increasing the production of this pharmacological active compound.

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Correspondence to Alexandre J. Silva da Góes.

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Author contributions

AJSG designed the study and the figures composed and edited; MSLS elaborated the manuscript and performed the experiment in vitro. AJA collected and registered the botanical material; VMBL and CMLM performed in vitro experiment; JAS and GGO performed the spectrometric analysis and characterized the isolated compound; RMFS and BSS critically revised the manuscript for clarity and intellectual content. All authors read and approved the final manuscript.

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de Lima Silva, M.S., de Santos, J.A., Alves, A.J. et al. Evaluation of the immunomodulatory effect against splenocytes of Balb/c mice of biflorin obtained from Capraria biflora by a new isolation method. Rev. Bras. Farmacogn. 29, 464–469 (2019). https://doi.org/10.1016/j.bjp.2019.01.010

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Keywords

  • Biflorin
  • Original method
  • Biological activity
  • Immunomodulatory effect