Simultaneous quantification of six flavonoids in four Scutellaria taxa by HPLC-DAD method

Abstract

The objective of this study was to develop and validate a new HPLC method to quantify several flavonoids in the methanol extract prepared from the aerial parts of four Scutellaria L. taxa from flora of Turkey. A simple, sensitive and precise reversed phase HPLC-DAD method was developed and validated for simultaneous determination of six main flavonoids; scutellarein 7-O-β-d-glucopyranoside, hispidulin 7-O-β-d-glucuronopyranoside, apigenin 7-O-β-d-glucopyranoside, hispidulin 7-O-β-d-glucopyranoside, luteolin and apigenin. All standard compounds showed a good linearity (R2 > 0.999) in a relatively wide concentration range (1–120 μg/ml). The limit of detection of the compounds was in the range of 0.016–1.883 μg/ml and the limit of quantification was in the range of 0.232–3.368 μg/ml. The recoveries of the selected compounds were calculated in the range of 92.20–107.93%. The amounts of flavonoids showed variation in the extracts. The developed method was found to be accurate, precise, reproducible, and can be successfully applied to identify and quantify the flavonoid composition of Scutellaria species.

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Correspondence to Hilal Bardakci.

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Authors contributions

HK contributed in collecting and identifying plant materials, herbarium sample preparation, study design, evaluation of results and critical reading of the manuscript. HBA prepared the working samples and standard solutions, performed the chromatographic analysis, method validation, evaluated the results and wrote the manuscript. ETA performed the chromatographic analysis and performed method validation studies. All authors have read the final manuscript and approved the submission.

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Bardakci, H., Acar, E.T. & Kırmızıbekmez, H. Simultaneous quantification of six flavonoids in four Scutellaria taxa by HPLC-DAD method. Rev. Bras. Farmacogn. 29, 17–23 (2019). https://doi.org/10.1016/j.bjp.2018.09.006

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Keywords

  • Flavonoids
  • HPLC-DAD
  • Scutellaria albida ssp. albida
  • Scutellaria hastifolia
  • Scutellaria orientalis ssp. pinnatifida
  • Scutellaria velenovskyi