The objective of this study was to develop and validate a new HPLC method to quantify several flavonoids in the methanol extract prepared from the aerial parts of four Scutellaria L. taxa from flora of Turkey. A simple, sensitive and precise reversed phase HPLC-DAD method was developed and validated for simultaneous determination of six main flavonoids; scutellarein 7-O-β-d-glucopyranoside, hispidulin 7-O-β-d-glucuronopyranoside, apigenin 7-O-β-d-glucopyranoside, hispidulin 7-O-β-d-glucopyranoside, luteolin and apigenin. All standard compounds showed a good linearity (R2 > 0.999) in a relatively wide concentration range (1–120 μg/ml). The limit of detection of the compounds was in the range of 0.016–1.883 μg/ml and the limit of quantification was in the range of 0.232–3.368 μg/ml. The recoveries of the selected compounds were calculated in the range of 92.20–107.93%. The amounts of flavonoids showed variation in the extracts. The developed method was found to be accurate, precise, reproducible, and can be successfully applied to identify and quantify the flavonoid composition of Scutellaria species.
Awad, R., Arnason, J., Trudeau, V., Bergeron, C., Budzinski, J., Foster, B., Merali, Z., 2003. Phytochemical and biological analysis of skullcap (Scutellaria lateriflora L): a medicinal plant with anxiolytic properties. Phytomedicine 10, 640–649.
Bardakci, H., Skaltsa, H., Milosevic-Ifantis, T., Lazari, D., Hafjipavlou-Litina, D., Yesilada, E., Kirmizibekmez, H., 2015. Antioxidantactivitiesofseveral Scutellaria taxa and bioactive phytoconstituents from Scutellaria hastifolia L. Ind. Crops Prod. 77, 196–203.
Bardakci, H., Türköz Acar, E., Kırmızıbekmez, H., 2018. Simultaneous quantification of six phenylethanoid glycosides in some Turkish Scutellaria species by a new HPLC-DAD method. Nat. Prod. Res. 18, 1–4.
Baytop, T., 1999. Türkiye’ de Bitkiler ile Tedavi, second ed. Nobel Tıp Kitapevi, Istanbul.
Brock, C., Whitehouse, J., Tewfik, I., Towell, T., 2012. The use of Scutellaria lateriflora: a pilot survey amongst herbal medicine practitioners. J. Herb. Med. 2, 34–41.
Dong, Y., Leu, Y., Chien, K., Yu, J., 2009. Separation and determination of low abundant flavonoids in Scutellaria baicalensis Georgi by micellar electrokinetic capillary electrophoresis. Anal. Lett. 42, 1444–1457.
Duman, H., 2000. Scutellaria L. In: Güner, A., Özhatay, N., Ekim, T., Bas¸er, K.H.C. (Eds.), Flora of Turkey and Aegean Islands, vol. 11. Edinburgh University Press, Edinburgh, pp. 198–199.
Edmonson, J.R., 1972. Scutellaria L. In: Davis, P.H. (Ed.), Flora of Turkey and Aegean Islands, vol. 7. Edinburgh University Press, Edinburgh, pp. 78–100.
Gao, J., Medina, A., Pendry, B., Hughes, M., Webb, G., Corcoran, O., 2008. Validation of a HPLC method for flavonoid biomarkers in skullcap (Scutellaria) and its use to illustrate wide variability in the quality of commercial tinctures. J. Pharm. Pharm. Sci. 11, 77–87.
ICH, 2005. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, Validation of Analytical Procedures: Text and Methodology Q2 (R1).
Li, G., Ma, J., Chen, Y., Tian, Q, Shen, Y., Wang, X., Chen, B., Yao, S., 2009. Investigation of flavonoid profile of Scutellaria bacalensis Georgi by high performance liquid chromatography with diode array detection and electrospray ion trap mass spectrometry. J. Chromatogr. A 1216, 4809–4814.
Matsa, M., Gousiadou, C., Bardakci Altan, H., Kırmızıbekmez, H., Skaltsa, H., 2013. Secondary metabolites from Scutellaria velenovskyi Rech (Poster Presentation). 61st International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research, Münster, Germany. Planta Med. 79, P173.
McPolin, O., 2009. Validation of Analytical Methods for Pharmaceutical Analysis. Mourne Training Services, Ireland.
Paton, A., 1990. The phytogeography of Scutellaria L. Notes R. Bot. Gard. Edinb. 46, 345–359.
Qiao, S., Shi, R., Liu, M., Zhang, C., Yang, W., Shi, X., Jiang, X., Wang, C., Wang, Q, 2011. Simultaneous quantification of flavonoids and phenolic acids in Herba Scutellariae barbatae and its confused plants by high performance liquid chromatography-tandem mass spectrometry. Food Chem. 129, 1297–1304.
Shabir, G.A., 2003. Validation of high-performance liquid chromatography methods for pharmaceutical analysis: understanding the differences and similarities between validation requirements of the US Food and Drug Administration, the US Pharmacopeia and the International Conf. J. Chromatogr. A 987, 57–66.
Shang, X., He, X., He, X., Li, M., Zhang, R., Fan, P., Zhang, Q, Jia, Z., 2010. The genus Scutellaria an ethnopharmacological and phytochemical review. J. Ethnophar-macol. 128, 279–313.
Yu, K., Gong, Y., Lin, Z., Cheng, Y., 2007. Quantitative analysis and chromatographic fingerprinting for the quality evaluation of Scutellaria baicalensis Georgi using capillary electrophoresis. J. Pharm. Biomed. Anal. 43, 540–548.
HK contributed in collecting and identifying plant materials, herbarium sample preparation, study design, evaluation of results and critical reading of the manuscript. HBA prepared the working samples and standard solutions, performed the chromatographic analysis, method validation, evaluated the results and wrote the manuscript. ETA performed the chromatographic analysis and performed method validation studies. All authors have read the final manuscript and approved the submission.
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Bardakci, H., Acar, E.T. & Kırmızıbekmez, H. Simultaneous quantification of six flavonoids in four Scutellaria taxa by HPLC-DAD method. Rev. Bras. Farmacogn. 29, 17–23 (2019). https://doi.org/10.1016/j.bjp.2018.09.006
- Scutellaria albida ssp. albida
- Scutellaria hastifolia
- Scutellaria orientalis ssp. pinnatifida
- Scutellaria velenovskyi