Various traditional systems of medicine enlightened the importance of Premna microphylla Turcz., Lamiaceae, medicinally. The present study was carried out to provide a scientific basis of the identification and the authenticity of P. microphylla with the help of pharmacognostical parameters, which is not done before. Roots, stems, and leaves of P. microphylla were collected for pharmacognostical studies involving macros, microscopic evaluation, physicochemical parameters analysis like fluorescence analysis and thin layer chromatography, in addition with DNA barcodes of internal transcribed spacer and psbA-trnH regions. Transverse section of root indicated the presence of stone cell bands. Transverse section of stem showed the presence of stone cells and vessels. Transverse section of leaf midrib revealed the presence of shaft type of porosity. Microscopic studies of powder revealed the presence of cork cells, fibers, vessels, nonglandular hairs, stone cells and glandular scale cells. Thin layer chromatography of the extract revealed the presence of oleanolic acid in P. microphylla with specific Rf values. Identification through DNA barcode showed the sequence of internal transcribed spacer region was novel while the sequence of psbA-trnH region displayed no differences from known sequence. The observations confirmed that P. microphylla has an obvious pharmacognostical characteristics, which will be useful toward providing a reliable basis for identification, purity, quality and classification of the plant.
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GQ Tang, XJ Lin, JN Li and R Li assisted in carrying out the laboratory work. GQ Tang collected the plant material, worked on laboratorial tests, as well as wrote and formatted the paper. XJ Lin performed the scanning electron microscopy analysis and assisted with writing. D Wang and SG Ji created the project, supervised the study, the writing and review of the manuscript. All the authors have read the final manuscript and approved the submission.
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Tang, G., Lin, X., Li, J. et al. Pharmacognostical studies of Premna microphylla. Rev. Bras. Farmacogn. 28, 520–526 (2018). https://doi.org/10.1016/j.bjp.2018.07.002
- DNA barcode